To assess the contribution of ppGpp in antibiotic tolerance to quinolone Pseudomonas aeruginosa, knockout mutants genes involved or linked with stringent response, such as relA, spoT and dksA, were constructed investigated for their susceptibility quinolones. The survival dksA presence 8 microg/ml ofloxacin 1 ciprofloxacin shown be approximately 20-180 10-40 times respectively, higher than same wild type strain. intracellular levels determined high performance liquid chromatography (HPLC)...

<ns3:p>Introduction Calcium hydroxide (Ca(OH)2) is the material of choice for pulp therapy. However, Ca(OH)2 has drawbacks such as toxicity, poor sealing, and tunnel defect formation. Alternative materials have been developed to provide more biocompatible with better dentin formation ability. The objective this study was evaluate effect composites containing gelatin (G), chitosan (CH), tetraethyl orthosilicate (TEOS), Ca(OH)2, namely G-CH-TEOS-Ca (OH)2 (Extended data) on inflammation dental...

<ns3:p>Introduction Calcium hydroxide (Ca(OH)<ns3:sub>2</ns3:sub>) is the material of choice for pulp therapy. However, Ca(OH)<ns3:sub>2</ns3:sub> has drawbacks such as toxicity, poor sealing, and tunnel defect formation. Alternative materials have been developed to provide more biocompatible with better dentin formation ability. The objective this study was evaluate effect composites containing gelatin (G), chitosan (CH), tetraethyl orthosilicate (TEOS), Ca(OH)<ns3:sub>2</ns3:sub>, namely...

This study aims to compare the regenerative effects of various by-products human leukocyte platelet-rich fibrin (L-PRF), including L-PRF exudate, concentrated PRF (C-PRF), and a mixture two, with hyaluronic acid (HA) specifically for interdental papillae reconstruction. The was obtained by centrifuging 10 mL blood in fixed-angle centrifuge at 2,700 rpm 12 minutes. After centrifugation, layer separated, platelet counts were performed. An vivo conducted using Sprague-Dawley rats subjected...

Green leafy vegetables such as arugula are rich in nitrates that support oral health, yet their effects on microbial balance, especially smokers, remain unclear. This study evaluated the survival and activity of nitrate-reducing bacteria (NRB; Veillonella spp. Rothia spp.) biofilm exposed to nitrate-containing juice (3.25 or 6.25 μM). The proportions NRB were compared with periodontopathogens ( Porphyromonas gingivalis Fusobacterium nucleatum ). Using quantitative real-time PCR (qPCR), we...

Persepsi kebersihan gigi dan oral memiliki dampak besar pada harapan hidup di seluruh dunia. Namun, negara-negara dengan pendapatan sedang, beban meningkat secara tidak proporsional. Prob-lema kesehatan mulut Indonesia merupakan problem yang mempengaruhi populasi sebanyak 57.6%. Studi ini adalah studi semi-eksperimental dilakukan 16 Desember 2024 Ta’lim Sa-bilu Taubah Gas Iqdam, Blitar, Jawa Timur, Indonesia. Metode sosialisasi offline (tatap muka) menggunakan campuran media presentasi...

Penelitian ini bertujuan untuk mengkaji dampak ekuitas merek, desain produk, dan gaya hidup terhadap keputusan pembelian ponsel iPhone di kalangan generasi muda yang tinggal Kecamatan Gajah, Demak. Sebanyak 60 responden dipilih menggunakan teknik purposive sampling menjadi sampel dalam penelitian ini. Data dikumpulkan melalui kuesioner, analisis data dilakukan dengan SPSS versi 30. Uji diterapkan meliputi uji kualitas (validitas reliabilitas), asumsi klasik (normalitas, heteroskedastisitas,...

The aim of this study was to determine whether Actinobacillus actinomycetemcomitans lipopolysaccharide (LPS‐ A. ) could stimulate a murine macrophage cell line (RAW264.7 cells) produce nitric oxide (NO). cells were treated with LPS‐ or Escherichia coli LPS Ec for 24 h. effects N G ‐monomethyl‐L‐arginine (NMMA), polymyxin B and cytokines (IFN‐γ, TNF‐α, IL‐4 IL‐12) on the production NO also determined. role protein tyrosine kinase, kinase C microtubulin organization assessed by incubating...

The alternative sigma factor sigma54 has been implicated in diverse functions within the cells. In this study, we have constructed an rpoN mutant of Pseudomonas aeruginosa and investigated its importance as a target for antimicrobial agents, such quinolones carbapenems. stationary-phase cells displayed survival rate approximately 15 times higher than that wild-type presence stationary phase led to substantial production pyoverdine by P. mutant. Pyoverdine synthesis correlated with decreased...

Pseudomonas aeruginosa is a Gram-negative bacterium and causes respiratory infection especially in elderly patients. Royal jelly has been used worldwide as traditional remedy nutrient; however, the effect against P. unclear. The aim of this study was to analyze antibacterial, antiadherent, anti-inflammatory effects royal aeruginosa. Wild-type strain PAO1 clinical isolates were for antibacterial assay antiadherent abiotic surface epithelial cells, which are pharynx (Detroit 562) lung...

Inflamasi merupakan respon alami tubuh terhadap adanya kerusakan jaringan. Salah satu medikamen untuk mengatasi inflamasi adalah antiinflamasi non steroid (AINS). Penggunaan AINS mempunyai beberapa efek samping dan dalam hal penggunaan tanaman obat dinilai lebih aman. Rosela salah yang potensi sebagai antiinflamasi. Penelitian ini bertujuan mengetahui pemberian sistemik ekstrak etanolik rosela ekspresi COX-2 jumlah neutrofil fase pada proses penyembuhan luka. Bunga didapatkan dari perkebunan...

The bone of yellowfin tuna (Thunnus albacares) contains high calcium and phosphor can be synthesized into hydroxyapatite (HA). Due to its mineral content similarity in chemical composition with human hard tissue, HA may have potency as a pulp capping material. aim this vitro study was evaluate the viability alkaline phosphatase (ALP) activity dental cells after exposure from (THA). Pulp were isolated human-impacted third molar. To cells, cultured exposed various concentrations (6.25 200...

Private organizations often face stronger competitive pressures and place the burden of coping on their employees. However, toxic work environments unhealthy workloads can negatively impact workers. Workers spend most lives in environment, so having workers with low job satisfaction has an workers' sustainability psychological health. The objective this study is to examine a workplace heavy among private sector Indonesian province Yogyakarta Special Region. Emotional exhaustion will be...

<ns3:p><ns3:bold>Background: </ns3:bold><ns3:italic>Pseudomonas aeruginosa</ns3:italic>, a multidrug-resistant Gram-negative bacterium, produces pyocyanin, virulence factor associated with antibiotic tolerance. High concentrations of royal jelly have an antibacterial effect, which may potentially overcome resistance. However, in some cases, tolerance can occur due to prolonged stress low-dose agents. This study aimed investigate the effect subinhibitory on bacterial growth, pyocyanin...

<ns3:p><ns3:bold>Background: </ns3:bold><ns3:italic>Pseudomonas aeruginosa</ns3:italic>, a multidrug resistant Gram-negative bacterium, produces pyocyanin, virulence factor associated with antibiotic tolerance. High concentrations of royal jelly have an antibacterial effect, which may the potential to overcome resistance. However, in some cases, tolerance can occur due prolonged stress low-dose agents. This study aimed investigate effect subinhibitory on bacterial growth and pyocyanin...

Cell morphological changes on HeLa cells after Curcuma longa etanolik extract exposure. is mostly found in the areas with tropical and sub-tropical climate, one of original plants Southeast Asia. In Indonesia, curcuma can be almost all regions areas. Curcumin, which curcuma’s main constituent, a potent anti oxidant. Previous studies reported that may decrease growth cancer by interfering cell proliferation, causing apoptosis; however, mechanism apoptosis remains unclear. The purpose this...

Red onion skin (Allium cepa var ascalonicum) contains various ingredients that may function as antibacterial agents against microorganisms, well anti-inflammatory and immunomodulator for host cells, such macrophages. Pseudomonas aeruginosa found in the oral cavity is commensal bacteria turn into opportunistic pathogen by utilizing its virulence factors motility adhesion to cell. The purpose of this study was investigate effect red-onion-skin ethanolic extract towards P. ATCC 9027 on ability,...

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium, which could aggressively infect immunocompromised patients and thus, cause high mortality rate. In addition, P. in oropharynx be aspirated ventilator associated pneumonia. Royal jelly one of bee’s products that has been used for therapeutic needs including antibacteria. Adherence factor were flagelum, pili lectin. The aim the study was to determine effect royal adhesion. Suspension (ATCC® 27853) incubated at 37 °C 18 h....

Introduction: Some analgesic drugs may have adverse effects on bone remodelling and, thus, orthodontic tooth movement rate (OTM). GV-0 is synthesized by reacting vanillin and cyclopentanone catalyzed in acidic condition, it has been revealed as a selective COX-2 inhibitor. This study was aimed to investigate the effect of pentagamavunon-0 (PGV-0), one curcumin analogues, OTM. Methods: conducted 50 male Wistar rats (350-450 g) which were randomly divided into five groups (n = 10 each): 1) no...