A5101717362- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Viral-associated cancers and disorders
- Heavy metals in environment
- Heavy Metal Exposure and Toxicity
- Lymphoma Diagnosis and Treatment
- Online and Blended Learning
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- Cancer Risks and Factors
- Online Learning and Analytics
- Gastrointestinal Tumor Research and Treatment
- Innovative Teaching and Learning Methods
- Global Cancer Incidence and Screening
- Renal and related cancers
- Mercury impact and mitigation studies
- Cardiac Valve Diseases and Treatments
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Colorectal Cancer Screening and Detection
- Chronic Lymphocytic Leukemia Research
- Renin-Angiotensin System Studies
- Breast Cancer Treatment Studies
University of Cape Town
2014-2025
National Health Laboratory Service
2014-2024
University of South Africa
2016-2020
Pioneer (United States)
2018
University of Venda
2015
University of KwaZulu-Natal
1998-2010
Pfizer (United States)
2006
Harvard University
1996
The presence of the apolipoprotein E-epsilon4 (APOE-epsilon4) allele is reported to be associated with poor outcome after traumatic brain injury (TBI). This study was performed determine if APOE-epsilon4 influenced in a cohort black patients TBI who had homogeneous neuropathological findings.Venous blood collected at time admission APOE genotype Zulu-speaking presented cerebral contusions. frequency allele's appearance correlated minimum 6 months follow up. Univariate and multivariate...
The study was conducted to assess the expression levels of epithelial mesenchymal transition (EMT) proteins (E-cadherin, N-cadherin, snail-1 and vimentin) miRNA-21. In addition, we correlated these data with clinicopathological features in Colorectal cancer.H&E slides from a total 59 formalin fixed paraffin embedded tissue blocks were examined by pathologist demarcate normal tumour regions. Immunohistochemical analysis mismatch repair (MLH1, MSH2 MSH6) EMT markers performed. miRNA-21...
Despite the success of antiretroviral therapy in HIV treatment, cervical cancer remains a leading malignancy HIV-infected women. Additionally, co-infection by and HPV further accelerates development. There are limited studies on role host somatic variations infected HIV-negative women with cancer. Therefore, this study aimed to investigate compare genetic variation biopsies obtained from intraepithelial neoplasia 3 understand genomic landscape. The distribution types was also investigated...
Few studies have investigated risk factor heterogeneity by molecular subtypes in indigenous African populations where prevalence of traditional breast cancer (BC) factors, genetic background, and environmental exposures show marked differences compared to European ancestry populations.
We studied the in vivo effects of sanguinarine a hypertensive rat model and its on AT1a mRNA expression kidney tissues. Rats received daily for 14 d 0.1 mg/kg (SangL) 0.3 (SangH), losartan 1 by weight (Los), or DMSO (Con). Blood pressures were monitored regularly urine volume sodium concentration was measured days 0, 7, 14. On day 15, animals anesthetized (sodium thiopentane, 50 mg/kg), blood samples aldosterone levels taken, kidneys removed expression. Los SangH groups showed reduced...
This study was undertaken to investigate microsatellite instability and allelic imbalance in a cohort of young patients (<35 yr) older (>50 with sporadic colorectal carcinomas the Kwa-Zulu Natal region South Africa.Normal tumor DNA isolated from formalin-fixed, paraffin-embedded tissue 32 <35 yr 50 >50 age. Tumors were staged using modified Astler-Coller classification. Fluorescent-based technology an automated sequencer (Alf Express Automated Sequencer) employed. CY5 labeled primers for...
Background: Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease that includes wide spectrum of lymphomas posing challenges in diagnosis and treatment. the most common subtype occurring older populations as well younger HIV-infected patients. Outcomes have improved with antiretroviral therapy use rituximab; however, it remains poorly treated resource-constrained countries. Aim: This study aimed to determine differences clinical features between HIV-negative HIV-positive DLBCL...
Microsatellite instability has been reported in a wide variety of cancer types. Inactivation or loss tumour suppressor genes shown to result cell cycle deregulation and neoplastic growth. We conducted microsatellite study using fluorescent-based DNA technology determine whether mutations the sequences deleted colorectal (DCC) gene, at 18q21.1, have any pathologic correlation prognostic significance nephroblastomas. Normal was isolated from 106 cases nephroblastoma standard proteinase K...
This study compares student performance in an undergraduate mathematics education course using aTraditional Learning Model and a Blended at the University of Technology. The control andexperimental groups consisted 150 students each group. Student examination scores from both wereanalyzed quantitatively. A random sample consisting 40 students, group, wasclinically interviewed qualitative analysis was performed. Theory Connectivism informed methodused to analyze data. quantitative indicates...
The mammalian beta-like globin gene family has served as an important model system for analysis of tissue- and developmental state-specific regulation. Although the activities a number regulatory proteins have been implicated in erythroid cell-specific transcription genes, mechanisms that restrict their expression to discrete stages development are less well understood. We previously identified novel element (PRE II) upstream from human embryonic (epsilon) synergizes with other sequences...
While fish is considered a healthy component of the human diet, consumption with high levels trace metals in their flesh constitutes public health risk as have been proven to be toxic.We investigated concentrations toxic elements seawater and also different body parts Trachurus capensis caught near Durban, South Africa, using inductively coupled plasma-atomic emission spectrometry (ICP-AES).The highest metal concentration was observed for Pb, followed by Zn.Significantly higher Mn were gills...
Background: Oesophageal cancer is the most common malignancy encountered in South African males, especially Eastern Cape and surrounding region of Africa. There are a number risk factors predisposing conditions that have been implicated aetiology disease. The tylosis oesophageal ( TOC ) gene, localised to small on chromosome 17q25, has shown be associated with squamous cell carcinoma. Aim: To investigate loss heterozygosity (LOH) microsatellite instability (MSI) locus. Methods: In 74...
Abstract BackgroundFew studies have investigated risk factor heterogeneity by molecular subtypes in indigenous African populations where prevalence of traditional breast cancer (BC) factors, genetic background, and environmental exposures show marked differences compared to European ancestry populations. MethodsWe conducted a case-only analysis 838 pathologically confirmed BC cases recruited from 5 groups public, faith-based private institutions across Kenya between March 2012 May 2015....
Microsatellite polymerase chain reaction (PCR) and fluorescent DNA technology was used to assess allelic imbalance (AI) or loss of heterozygosity (LOH) microsatellite instability (MSI) in chromosomes 2p, 3p, 5q, 18q esophagectomy specimens from 39 patients who had squamous carcinoma lived a high-incidence geographic location South Africa. The carcinomas were graded by conventional light microscopy staged using the tumor-node-metastasis (TNM)-Union Internationale Contre Le Cancer (UICC)...
The asialoglycoprotein receptor, which is abundantly and near exclusively expressed on hepatocytes, has received much attention in the design of non-viral hepatotropic DNA delivery systems. Thus, asialoglycoproteins hexopyranosyl ligands have been coupled to DNA-binding cationic polymers liposomes assembly complexes intended for uptake by liver parenchymal cells. aim study was construct a hepatocyte-targeted multimodular liposome-based transfecting complex, biotin-streptavidin interaction...
This study investigated the use of pressure cooking with different retrieval solutions at variable pHs to determine optimum protocol for extraction high quality DNA from formalin fixed paraffin embedded (FFPE) tissues. Further, oxidation effect on archived FFPE tissues in relation age tissue blocks was also explored. The main aim investigate this technique 11 gastric cancers. design our based principles antigen using a conventional cooker improve extracted In addition cancers, lymphoma,...