The recommendation from the 2009 World Health Organization guidelines for managing dengue suggests that patients with any warning sign can be hospitalized observation and management. We evaluated utility of using signs to guide hospital admission predict disease progression in adults. conducted a prospective cohort study January 2010 September 2012. Daily demographic, clinical laboratory data were collected adult patients. Warning recorded. proportion admitted current criteria was compared....

Studies on severe neutropenia in dengue are scarce, and its clinical significance is uncertain. We analyzed a cohort of 1,921 reverse transcription polymerase chain reaction-confirmed adult patients admitted to the Communicable Disease Center Singapore between 2005 2008. Time trend analyses for daily absolute neutrophil counts (ANCs) were done using Bayesian hierarchical Markov models. found that neutropenia, defined as ANC ≤ 0.5 × 10 9 /L, was 11.8% with median duration 1 day. nadir...

Serum amyloid A1 (SAA1) is an acute response protein, which mainly produced by the liver, during infection. However, it remains unknown whether SAA1 can be in human fetal membranes where able to elicit events pertinent labor initiation. We demonstrated that was expressed fibroblasts and epithelium of amnion trophoblasts chorion. Further study showed production augmented interleukin-1β (IL-1β) cortisol alone synergistically, turn induced expression IL-1β, interleukin-6 (IL-6),...

Deep eutectic solvents (DESs) have gained much attention in the fabrication of advanced nanoelectrocatalysts due to their amazing template function. However, stabilizing function for easily hydrolyzed inorganic nanomaterials is rarely studied. Here, a DES-mediated strategy was reported synthesize octahedral Ni–Co precursor (NiCo–NH3 complex), which could be directly transformed into NiCo2O4 nanooctahedrons after thermal decomposition. The NiCo–NH3 shape achieved with crystallization choline...

Abstract Background Preeclampsia, especially early-onset preeclampsia (EO-PE), is a pregnancy complication that has serious consequences for the health of both mother and fetus. Although abnormal placentation due to mitochondrial dysfunction speculated contribute development EO-PE, underlying mechanisms have yet be fully elucidated. Methods The expression localization Siglec-6 in placenta from normal pregnancies, preterm birth EO-PE patients were examined by RT-qPCR, Western blot IHC....

Abstract Amnion‐derived prostaglandin E2 (PGE2) and cortisol are key to labor onset. Identification of a common transcription factor driving the expression both cyclooxygenase‐2 (COX‐2) 11β‐hydroxysteroid dehydrogenase 1 (11β‐HSD1), enzymes in their production, may hold treatment pre‐term labor. Here, we have found that CCAAT enhancer binding protein δ (C/EBPδ) is such which underlies feed‐forward induction COX‐2 11β‐HSD1 by own products PGE2 human amnion fibroblasts so production would be...

Background Dengue is an important viral infection with different presentations. Predicting disease severity in triaging patients requiring hospital care. We aim to study the value of proteinuria predicting development dengue hemorrhagic fever (DHF), utility urine dipstick test as a rapid prognostic tool. Methodology and principal findings Adult undifferentiated (n = 293) were prospectively enrolled at Infectious Disease Research Clinic Tan Tock Seng Hospital, Singapore from January August...

Rupture of fetal membranes (ROM) can initiate parturition at both term and preterm. Collagen III in the compact layer amnion contributes to tensile strength membranes. However, upstream signals triggering collagen degradation remain mostly elusive. In this study, we investigated role cortisol regenerated by 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) human fibroblasts with an aim seek novel targets for prevention preterm premature ROM (pPROM)-elicited birth. Human tissue cultured explants...

Serum amyloid A1 (SAA1) is an acute phase protein produced mainly by the liver to participate in immunomodulation both sterile and non-sterile inflammation. However, non-hepatic tissues can also synthesize SAA1. It remains be determined whether SAA1 synthesized locally placenta participates parturition via eliciting inflammatory reactions. In this study, we investigated issue using human a mouse model. We found that mRNA expression placental villous trophoblasts, which was increased upon...

Purpose: The current study aimed to explore the central mechanism of primary dysmenorrhea (PD) by investigating alterations in resting state amplitude low-frequency fluctuation (ALFF) and regional cerebral blood flow (CBF) between PD patients healthy controls (HCs). Patients methods: A total 34 female subjects including 20 14 HCs underwent resting-state functional magnetic resonance imaging (rs-fMRI) arterial spin labeling technique (ASL) MRI during menstrual phase. Subsequently, differences...

Abstract Pyruvate dehydrogenase kinase (PDK) is known as a gatekeeper directing the carbon flux into glycolysis via inhibition of pyruvate complex. During syncytialization placental trophoblasts, both ATP production and oxygen consumption are increased to meet enhanced energetic demands by syntiotrophoblasts. We hypothesized that down-regulation PDK expression may play central role in switch from oxidative phosphorylation (OXPHOS) during syncytialization. By using primary human we...

Phosphorylation of the transcription factors cyclic adenosine monophosphate response element-binding protein (CREB) and signal transducer activator 3 (STAT3) by kinase A (PKA) is required for cortisol-induced production cyclooxygenase-2 (COX-2) prostaglandin E2 (PGE2) in human amnion fibroblasts, which critically mediates parturition (labor). We found that PKA was confined nucleus A-kinase-anchoring 95 (AKAP95) fibroblasts this localization key to expression PTGS2, gene encoding COX-2....

Preterm premature rupture of fetal membranes precedes 30–40% preterm births. Activation matrix metalloproteases (MMPs) is the one major causes extracellular (ECM) degradation in membrane rupture. Increased cortisol, regenerated by 11β-hydroxysteroid dehydrogenase 1 amnion at parturition, known to participate a number parturition-pertinent events. However, whether cortisol has role regulation MMPs not known. Here, we addressed this issue using human tissue, most tensile layer membranes....

The electrochemical hydrogen evolution reaction (HER) is a promising strategy for production of hydrogen; however, it still restricted by appropriate efficient and low-cost electrocatalysts. Herein, the first time, n-octylammonium hypophosphite, kind protic ionic liquid (IL), was used as new phosphorus source manufacture nickel metaphosphate (Ni2P4O12) In contrast to traditional multi-step fabrication processes, hypophosphite acted both reactant solvent synthesize Ni2P4O12 one-step...

A hybrid catalyst of crystalline Fe₂O₃ with amorphous FeS was designed by a DES approach along subsequent annealing process, achieving high-efficiency NRR electrocatalyst.

Abstract Background The human amnion is an intrauterine tissue which involved in the initiation of parturition. In-depth understanding gene expression signatures individual cell types with respect to membrane rupture at parturition may help identify crucial initiators for development specific strategies prevent preterm birth, a leading cause perinatal mortality. Results Six major were revealed including epithelial cells, fibroblasts and immunocytes as well three other expressing dual markers...

The first steps of vision take place in the ciliary outer segment compartment photoreceptor cells. protein composition segments is uniquely suited to perform this function. most abundant among these proteins visual pigment, rhodopsin, whose trafficking involves intraflagellar transport (IFT). Here, we report three major findings from analysis mice which was acutely impaired by conditional knockouts IFT-B subunits. First, demonstrate existence a sorting mechanism whereby mislocalized...

Problem Rupture of fetal membranes is a crucial event at parturition, which preceded by extensive extracellular matrix (ECM) remodeling. Our recent studies have demonstrated that the human are capable de novo synthesis serum amyloid A1 (SAA1), an acute phase protein, and abundance SAA1 in amnion was increased parturition. However, exact role parturition remains to be established. Method study The effects on collagenases lysyl oxidase, enzyme cross‐links collagens, were investigated culture...