A5077154243- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- Animal Genetics and Reproduction
- Environmental Toxicology and Ecotoxicology
- Phytochemicals and Antioxidant Activities
- Fish Biology and Ecology Studies
- Probiotics and Fermented Foods
- Reproductive Biology and Fertility
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Estrogen and related hormone effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Marine Biology and Environmental Chemistry
- Physiological and biochemical adaptations
- Crustacean biology and ecology
- Protein Hydrolysis and Bioactive Peptides
- Hypothalamic control of reproductive hormones
- Phytoestrogen effects and research
- Nuts composition and effects
- Natural Antidiabetic Agents Studies
- Gut microbiota and health
- melanin and skin pigmentation
National Institute of Fisheries Science
2007-2022
Kangwon National University
2019
Busan Development Institute
2015
Rural Development Administration
2010
Institute of Crop Science
2010
The University of Tokyo
2009
Korea Research Institute of Bioscience and Biotechnology
2008
We prepared monoclonal antibodies (mAbs) against a recombinant tethered follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica that was produced in Escherichia coli. Positive hybridomas (clones eFA-C5, eFA-C10, eFA-C11, eFA-C12, eFA-C13, and eFB-C14) were selected by using the FSH antigen ELISA, anti-eel mAbs purified culture supernatants performing affinity chromatography. Three of 6mAbs characterized their isotypes identified as IgG2b (eFA-C5 eFA-C11) IgG1 (eFB-C14). In...
Reproductive activities of salmonids are synchronized by changes in photoperiod, which control the endocrine system via brain-pituitary-gonadal axis. Gonadotropin-releasing hormone (GnRH) brain regulates synthesis and release pituitary gonadotropins (GTHs; FSH LH). LH turn stimulate production sex steroids for oocyte growth maturation-Inducing steroid hormones maturation ovulation, respectively, female salmonids. To clarify effects long-term photoperiod manipulations on reproductive activity...
Eel follicle-stimulating hormone (eelFSH) is composed of a common α-subunit and specific β-subunit, both which contain two N-linked carbohydrate residues. We characterized the biologically active single chains by fusing to carboxyl terminal region eelFSH β-subunit. Expression vectors were constructed biological activity recombinant hormones (rec-hormones) was using Chinese hamster ovary (CHO) K1 cells expressing receptor gene. Mutagenesis individual double glycosylated sites performed...
Tyrosinase는 피부의 melanocytes에서 멜라닌 생성을 촉진한다. 목단피의 미백효과를 알아보기 위하여 목단피 물추출물의 항산화 능력과 tyrosinase 활성 억제에 미치는 영향을 세포 모델계와 a cell-free 모델계에서 알아보았다. MDP의 라디칼 소거작용은 DPPH assay로 알아보았고, MDP는 높은 소거작용을 보여주었다. 세포내 활성은 RT-PCR, 효소활성을 마우스 B16 세포내에서 측정함으로써 24시간 물 추출물을 투여한 mRNA와 효소활성이 무처리군과 비교하여 현저히 감소하였다. 이와 같은 결과들은 항산화력이 높고, tyrosinase를 효과적으로 억제할 수 있는 이용하여 melanin 생합성을 미백 화장품 개발에 활용할 가능성을 제시하고 있다.
Abstract The present study demonstrates the changes in body weight (BW) and plasma sex steroid hormone profiles during artificial maturation induced by human chorionic gonadotropin (HCG) or salmon pituitary extract (SPE) injections cultured eel, Anguilla japonica, kept seawater for 3 months. In weekly SPE‐injected female group, BW was relatively stable vitellogenesis. Following induction of vitellogenesis, females exhibited a rapid increase BW, oocytes were observed to be migratory nucleus...
본 연구는 미나리(Oenanthe javanica, MNR), 율무(Coicis lachryma-jobi L. var., YM) 및 차전자(Plantaginis asiatica L, CJJ) 물 추출물이 지질 대사 관련 효소들인 lipoprotein lipase(LPL), acyl-CoA synthetase(ACS) 그리고 carnitine acetyltransferase(CAT) 활성에 유익한 영향력을 미치는지 알아보았다. LPL와 ACS는 비만 모델군 Zucker fatty 흰쥐(fa/fa)와 대조군인 lean 흰쥐(lean)의 부고환 지질과 간에서 각각 분리하였다. MNR이나 YM 추출물 처리는 일반(lean) 비만(fa/fa) 흰쥐로부터 분리한 LPL 활성을 현저하게 감소시켰다. MNR, CJJ물 10000 ppm을 처리했을 때 fa/fa 활성이 32.5%, 30.1% 22.8% 증가하였다. Lean ACS 대조군과 비교하여 추출물에서 현저히 증가하였고 MNR...
본 연구의 목적은 국내 주요 밤 5품종(단택, 대보, 석추, 옥광, 병고)의 전체과육, 흰과육 그리고 노란과육을 10개월 동안 냉장(4℃), 냉동(-10℃) 저장 중에 수분, 조단백질, 조지방, 비타민 C 당의 함량 변화를 알아보므로 국내산 밤의 이용가치를 높일 수 있는 저장방법 및 가공식품 개발을 위한 기초자료를 제공하는 것이다. 5품종의 전체과육 중수분함량은 49.9~57.4%였으며, 석추(57.4±0.2%)가 5품종 중 수분함량이 가장 높았고, 단택(49.9±0.1%)이 낮았다. 단택을 제외하고 시료의 흰과육이 전체과육보다 더 노란과육보다 흰과육에서 높았다. 냉장(4oC), 수분함량 보면 감소하는 경향이었다. 조단백질 함량은 3.3~4.2%였으며, 대보(4.2±0.2%)에서 함량이 단택, 병고를 10개월간 냉장 저장한 후 함량을 전과 비교한 결과 유의적으로 증가하였으나, 대보의 전체과육은 감소하였고, 노란과육은 변화가 없었다. 냉동 옥광의 전체시료에서만 조단백질이...
본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당 이용 대사 관련 주요 효소인 glucokinase(GK)와 acetyl CoA carboxylase(ACC)에 약용 식물 물 추출물들이 어떤 영향을 미치는지 알아보았다. α-Glucosidase 저해제는 당뇨 치료와 예방을 위해 사용되어져 오고 있다. 따라서 약용식물 추출물들의 α-glucosidase 저해 활성을 세포액 분획물을 GK과 ACC 알아보기 사용하였다. 실험에 사용된 식물들은 Lycium chinense(JGP), Discorea japonica Thunb.(SY), Pyrus pyrifolia(YSB), Cornus officinalis(SSY), Paeonia suffruticosa ANDR.(MDP), Cordyceps militaris(DCH) 그리고 Acanthopanax senticosus(GSO)였다. 지골피(JGP), 산약(SY), 야생배(YSB)...
A simple, cost‐effective, and greener HPLC method was investigated to determine 14 β‐lactams. To achieve this, various strategies were implemented such as the use of an aqueous mobile phase, ionic liquids, or alternative organic solvent. Preliminary experiments conducted using “one‐factor‐at‐a‐time” identify significant factors affecting chromatographic performance. The experimental design (central composite design) then applied thoroughly examine effects key exploit them optimal condition....
Lactic acid bacteria (LAB) are not only the most common probiotics in food and feed industry but also used as plant probiotics. Therefore, precise identification of LAB at species level is required. In this study, we compared three different methods, VITEK 2 ANC card, species-specific PCR, MALDI-TOF MS, to identify six (Lacticaseibacillus casei, Lacticaseibacillus paracasei, rhamnosus, Lactiplantibacillus plantarum, Lentilactobacillus buchneri, Limosilactobacillus fermentum) previously...
The present work examined the role of gonadotropin‐releasing hormone (GnRH) and dopaminergic drugs on secretion maturational gonado‐tropin (GTH II) in relation to testosterone (T) treatment. This study provides evidence that plasma GTH II levels are increased by T treatment precocious males, but not immature animal. In addition, GnRH analogue (GnRHa) alone significantly rainbow trout treated with T, as well T‐treated T‐untreated males. However, injection either dopamine (DA) or domperidone...
The glycoprotein hormone family consists of luteinizing (LH), follicle‐stimulating (FSH), and thyroid‐stimulating (TSH), which are secreted by the pituitary gland in all mammalian species, chorionic gonadotropin (CG), is placental trophoblast cells primates equids. These hormones non‐covalently associated α β subunits. Within a amino acid sequence subunit identical across encoded single gene. αβ dimer active form hormone, biological specificity conferred subunit. Also fish, duality (GTH),...
Abstract The control mechanism of gonadotropin‐releasing hormone (GnRH) on gonadotropin (GTH) release was studied using cultured pituitary cell or whole obtained from Testosterone (T) treated and immature rainbow trout. FSH not changed by salmon type GnRH (sGnRH), chiken‐II (cGnRH‐II), analogue ([des‐Gly10D‐Ala6] ethylamide) antagonist ([Ac‐3, 4‐dehydro‐Pro1, D‐p‐F‐Phe2, D‐Trp3,6] GnRH) in cells T‐treated fish. Indeed, also altered sGnRH pituitary. All tested drugs had no effect the LH both...
In the present study, we investigated effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture vitro and sex steroid hormones vivo Japanese Anguilla japonica. To study rec-GTHs estradiol-17β (E2) production tissues, tissues were incubated with different doses rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing (rec-LH). The results revealed that E2 levels rec-FSH (0.1, 0.5, 1 μg/mL)- rec-LH (0.1 0.5 μg/mL)-treated groups significantly...
Cytochrome P450 (CYP1A) gene expression in the liver and sex steroid levels plasma were investigated olive flounder (Paralichthys olivaceus) exposed to tributyltin (TBT) benzo[a]pyrene (BaP). We constructed a cDNA library cloned 230-base sequence encoding partial CYP1A DNA. The level was estimated using northern blotting. Hepatic mRNA fish injected with BaP at 10 mg/kg body weight (b.w.) increased for 48 h after injection. However, both TBT b.w. showed no significant changes h. Plasma...