A5031936876- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Autophagy in Disease and Therapy
- Metabolomics and Mass Spectrometry Studies
- COVID-19 Clinical Research Studies
- Cardiac Ischemia and Reperfusion
- Anesthesia and Neurotoxicity Research
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Anesthesia and Sedative Agents
- Congenital heart defects research
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Tissue Engineering and Regenerative Medicine
- SARS-CoV-2 detection and testing
- Bone and Dental Protein Studies
- Muscle Physiology and Disorders
- COVID-19 Impact on Reproduction
- Cardiomyopathy and Myosin Studies
- Amino Acid Enzymes and Metabolism
- Advanced Biosensing Techniques and Applications
- COVID-19 and healthcare impacts
- Diabetes and associated disorders
- Heart Failure Treatment and Management
- Genetics and Neurodevelopmental Disorders
Cedars-Sinai Smidt Heart Institute
2015-2025
Cedars-Sinai Medical Center
2015-2024
Center for Systems Biology
2015
Zero to Three
2014
University of British Columbia
2007-2011
Therapeutics Clinical Research
2011
University of Calgary
2005
Importance Certain individuals, when infected by SARS-CoV-2, tend to develop the more severe forms of Covid-19 illness for reasons that remain unclear. Objective To determine demographic and clinical characteristics associated with increased severity infection. Design Retrospective observational study. We curated data from electronic health record, used multivariable logistic regression examine association pre-existing traits a defined level required care: need hospital admission, intensive...
Accurate knowledge of retention time (RT) in liquid chromatography-based mass spectrometry data facilitates peptide identification, quantification, and multiplexing targeted discovery-based workflows. Retention prediction is particularly important for analysis emerging data-independent acquisition (DIA) experiments such as SWATH-MS. The indexed RT approach, iRT, uses synthetic spiked-in standards (SiRT) to set a unit-less scale, allowing normalization between different samples...
Accurate discovery assay workflows are critical for identifying authentic circulating protein biomarkers in diverse blood matrices. Maximizing the commonalities proteomic between different biofluids simplifies approach and increases likelihood reproducibility. We developed a workflow that can accommodate 3 blood-based proteomes: naive plasma, depleted plasma dried blood.
Abstract The global scientific response to COVID 19 highlighted the urgent need for increased throughput and capacity in bioanalytical laboratories, especially precise quantification of proteins that pertain health disease. Acoustic ejection mass spectrometry (AEMS) represents a much-needed paradigm shift ultra-fast biomarker screening. Here, quantitative AEMS assays is presented, employing peptide immunocapture enrich (i) 10 acute phase (APR) protein markers from plasma, (ii) SARS-CoV-2...
We sought to determine the extent of SARS-CoV-2 seroprevalence and factors associated with across a diverse cohort healthcare workers.
Proteomic analysis on the scale that captures population and biological heterogeneity over hundreds to thousands of samples requires rapid mass spectrometry methods, which maximize instrument utilization (IU) proteome coverage while maintaining precise reproducible quantification. To achieve this, a short liquid chromatography gradient paired data acquisition can be used reproducibly quantify moderate set analytes. High-throughput profiling at limited depth is becoming an increasingly...
Abstract Background Certain individuals, when infected by SARS-CoV-2, tend to develop the more severe forms of Covid-19 illness for reasons that remain unclear. Methods We studied N=442 patients who presented with laboratory confirmed our U.S. metropolitan healthcare system. curated data from electronic health record, and used multivariable logistic regression examine association pre-existing traits a severity defined level required care: need hospital admission, intensive care, intubation....
Patients with diabetes experience increased cardiovascular complications after cardiac surgery. Hyperglycaemia predicts mortality myocardial infarction and may influence risk in humans. Impaired prosurvival phosphatase tensin homologue on chromosome 10 (PTEN)-Akt signalling could be an important feature of the diabetic heart rendering it resistant to preconditioning. This study was designed evaluate for differences relationships PTEN-Akt-related baseline glycaemic control marker type 2...
BackgroundStress responses within the β cell have been linked with both increased death and accelerated immune activation in type 1 diabetes (T1D). At present, information on timing scope of these as well disease-related changes islet protein expression during T1D development is lacking.MethodsData independent acquisition-mass spectrometry was performed islets collected longitudinally from NOD mice NOD-SCID rendered diabetic through T adoptive transfer.FindingsIn female at 10, 12, 14 weeks...
Despite advances in clinical proteomics, translating protein biomarker discoveries into use remains challenging due to the technical complexity of validation process. Targeted MS-based proteomics approaches such as parallel reaction monitoring (PRM) offer sensitive and specific assays for translation. In this study, we developed a multiplex PRM assay using Stellar mass spectrometry platform quantify 57 plasma proteins, including 21 FDA-approved proteins. Loading curves (11-points) were...
We have developed an automated cell-based workflow for the quantification of proteins by liquid chromatography-mass spectrometry (LC-MS) that facilitates large-scale perturbation studies carried out in a 96-well plate format and enables preparation one full approximately 4 h, showcasing high-throughput (HTP) concept. Cells were grown lysed via ultrasonication. Proteins subsequently solubilized, extracted, processed into tryptic peptides 2 h before being acquired data-independent acquisition...
The extremely limited regenerative potential of adult mammalian hearts has prompted the need for novel cell-based therapies that can restore contractile function in heart disease. We have previously shown mixed fetal cells were naturally found migrating to injured maternal heart. Exploiting this intrinsic mechanism led current hypothesis Caudal-type homeobox-2 (Cdx2) placenta may represent a cell type cardiac regeneration. Using lineage-tracing strategy, we specifically labeled fetal-derived...
Osteopontin (OPN; also known as SPP1), an immunomodulatory cytokine highly expressed in bone marrow-derived macrophages (BMMΦ), is to regulate diverse cellular and molecular immune responses. We previously revealed that glatiramer acetate (GA) stimulation of BMMΦ upregulates OPN expression, promoting anti-inflammatory, pro-healing phenotype, whereas inhibition triggers a pro-inflammatory phenotype. However, the precise role macrophage activation state unknown.
B-type Natriuretic Peptide (BNP) is a biologically active circulating hormone. Plasma concentrations of BNP are routinely used in the diagnosis heart failure, and intravenous infusion recombinant can be for failure treatment. Like many bioactive polypeptides, multiple plasma enzymes known to cleave BNP, as part CVD-B/D-HPP mandate, we sought develop technique capable profiling these catabolic processes plasma. We neutral-coated capillary electrophoresis-electrospray ionization (CESI)...
BackgroundAortic dilation, stiffening, and dissection are common potentially lethal complications of Marfan syndrome (MFS) Loeys-Dietz (LDS), which involve abnormal transforming growth factor beta (TGF-β) signalling. The relation aortic dimensions, stiffness, biomarker levels is unknown. objective this study was to measure TGF-β matrix metalloproteinase (MMP) levels, endothelial function in patients with MFS, compare MFS receiving different therapeutic regimens.MethodsThis a cohort 40 4 LDS...
ABSTRACT Importance Antibody testing is important for understanding patterns of exposure and potential immunity to SARS-CoV-2. Prior data on seroprevalence have been subject variations in selection individuals nature as well timing relation exposures. Objective We sought determine the extent SARS-CoV-2 seroprevalance factors associated with seroprevelance across a diverse cohort healthcare workers. Design Observational study workers, including serology participant questionaires. Participants...
Plasma is one of the most important and common matrices for clinical chemistry proteomic analyses. Data-independent acquisition (DIA) mass spectrometry has enabled simultaneous quantitative analysis hundreds proteins in plasma samples support population disease studies. Depletion highest abundant a tool to increase proteome coverage, but this strategy can result nonspecific depletion protein subsets with which targeted interact, adversely affecting their analysis. Our work using an...