A5018009812- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Hepatitis C virus research
- Immune cells in cancer
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- Parasites and Host Interactions
- Hepatitis B Virus Studies
- Nigella sativa pharmacological applications
- Hematopoietic Stem Cell Transplantation
- Reproductive System and Pregnancy
- Chemotherapy-induced organ toxicity mitigation
- Research on Leishmaniasis Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bioactive Compounds and Antitumor Agents
- Systemic Lupus Erythematosus Research
- Tryptophan and brain disorders
- MicroRNA in disease regulation
- Dermatology and Skin Diseases
Tanta University
2016-2025
Tanta University Hospital
2018-2025
University Teaching Hospital
2025
University of Pennsylvania
2024
University Research Co (United States)
2023
Suez Canal University
2023
Creative Commons
2023
Mediclinic City Hospital
2020
University of Hafr Al-Batin
2020
University Hospital of Basel
2017
EpCAM (epithelial cell adhesion molecule) is a surface molecule that known to be highly expressed in colon and other epithelial carcinomas. involved cell-to-cell has been the target of antibody therapy several clinical trials. To assess value as novel for breast cancer gene therapy, we performed real-time reverse transcription-PCR quantify level mRNA expression normal tissue primary metastatic cancers. We found overexpressed 100- 1000-fold cancer. Silencing with short interfering RNA (siRNA)...
Dendritic cells (DCs) enhance the quality of anti-tumor immune response in patients with cancer. Thus, we posit that DC-based immunotherapy, conjunction toll-like receptor (TLR)-3 agonist poly-ICLC, is a promising approach for harnessing immunity against metastatic or locally advanced unresectable pancreatic cancer (PC). We generated autologous DCs from peripheral blood HLA-A2+ PC. were pulsed three distinct A2-restricted peptides: 1) human telomerase reverse transcriptase (hTERT, TERT572Y),...
Abstract Fibroblast growth factor (FGF) signaling has been implicated in the pathogenesis of pulmonary fibrosis. Mice lacking FGF2 have increased mortality and impaired epithelial recovery after bleomycin exposure, supporting a protective or reparative function following lung injury. To determine whether overexpression reduces bleomycin‐induced injury, we developed an inducible genetic system to express type II pneumocytes. Double‐transgenic (DTG) mice with doxycycline‐inducible human (...
Poly(I:C), a synthetic double-stranded RNA polymer and TLR3 agonist, can be used as vaccine adjuvant to enhance adaptive immunity. However, the antigen-specific CD8 T-cell response peptide vaccination poly(I:C) has not been clearly defined. Here, authors characterized specifically addressed hypothesis that antitumor To define response, established model based on adoptive transfer of T cells from OT-1 receptor transgenic mouse. In this model, with alone resulted in limited, transient...
Abstract Experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, is a Th1-mediated inflammatory demyelinating disease the CNS. AMP-activated protein kinase was reported recently to have anti-inflammatory activities by negatively regulating NF-κB signaling. In this study, we investigated prophylactic and therapeutic efficacy activator, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), in active passive EAE induced immunization with PLP139–151 or MOG35–55...
Constituents of the Nigella sativa seed are reported to possess potent antioxidant effects. Treatment with anticancer drugs such as cyclophosphamide (CTX) is associated significant toxicity due over-production reactive oxygen species, resulting in increased levels oxidative stress. The aim this study test whether or not N. L oil (NSO) its active ingredient, thymoquinone (TQ), can reduce CTX-induced toxicity. Male albino rats were treated intraperitoneal administration phosphate buffered...
Although cyclophosphamide (CTX) has been clearly shown to enhance active specific and adoptive immunotherapies, the mechanism(s) underlying these beneficial effects have not defined. To define impact of CTX preconditioning on antigen-specific CD8 T-cell response peptide vaccination, we used an transfer model based OT-1 receptor transgenic mouse. dramatically enhanced vaccination. Specifically, significantly expansion function responding T cells as demonstrated by flow cytometry cytokine...
Abstract Recent preclinical studies suggest that vaccination following adoptive transfer of CD8+ T cells into a lymphopenic host can augment the therapeutic antitumor responses transferred cells. However, mechanism by which microenvironment benefits Ag-specific cell remains elusive. We show herein induction lymphodepletion single 4 mg cyclophosphamide (CTX) treatment induces marked expansion immature dendritic (DCs) in peripheral blood on days 8–16 post-CTX (termed restoration phase). In...
Management of alopecia areata (AA) and androgenetic (AGA) is often challenging as patients may be resistant to currently available modalities treatment. The use stem cells a novel option for cases.To evaluate the safety efficacy autologous bone marrow derived mononuclear (including cells) compared follicular stems management cases AA AGA.This study included 40 (20 20 AGA patients), all were treated with single session intradermal injection (SCs) therapy. They divided into four groups...
Scorpion venom is a highly complex mixture of about 100–700 different components, where peptides are the major constituents with various biological and pharmacological properties including anticancer activities. In this study, efficacy Egyptian scorpion Androctonus amoreuxi has been evaluated. vitro, human breast cancer MCF-7 cell line was treated IC50 estimated. vivo studies, Ehrlich ascites carcinoma (EAC) cells were inoculated into CD-1 mice intraperitoneally to form liquid tumor or...
The systemic administration of IL-2 can act as a potent adjuvant for T cell-directed vaccine strategies. However, not only is the potentially toxic, but recent evidence suggests that it may also paradoxically limit duration and magnitude cytotoxic cell response. A recently identified cytokine, IL-15, shares many properties with provide preferential means augmenting responses. Although well characterized in vitro, there are few data on ability IL-15 to augment cell-mediated responses vivo. We...
Abstract Preconditioning a recipient host with lymphodepletion can markedly augment adoptive T cell therapy. However, the precise mechanisms involved are poorly understood. In recent study, we observed significant increase in circulating levels of dendritic cells (DCs; CD11c+CD11b+) during recovery from cyclophosphamide (CTX)-induced lymphodepletion. Herein, demonstrate that CTX-induced DC expansion was not altered by adjuvant chemotherapy or tumor burden but augmented coadministration...
The pathophysiology of severe coronavirus disease 2019 (COVID-19) is primarily a host immune interplay to virus invasion. therapeutic options have been explored either against hyperinflammation from dysregulated adaptive immunity or direct neutralization using antibodies convalescent plasma (CP) recovered patient. exchange (TPE) for removal excessive inflammatory cytokines has tried with success in COVID-19. We undertook this exploratory study evaluate safety and efficacy TPE followed by CP...
Although an immunomodulatory role for estrogens has long been demonstrated by experimental and clinical observations, the mechanism which exert their effect on T cells not clearly defined.In this study we analyzed effects of beta-estradiol (E2), at its contraceptive dose, delayed-type hypersensitivity (DTH) to purified protein derivatives (PPD) associated immune response in female mice.E2 treatment decreased PPD-specific DTH response, coincided with a decrease leukocytes numbers draining...