The U.S. National Cancer Institute has used a panel of 60 diverse human cancer cell lines (the NCI-60) to screen >100,000 chemical compounds for anticancer activity. However, not all important types are included in the panel, nor drug responses predictive clinical efficacy patients. We asked, therefore, whether it would be possible extrapolate from that rich database (or analogous ones other screens) predict activity or, matter, patients with tumors. address challenge by developing and...

Platelet-derived microparticles are the most abundant type of microparticle in human blood and contribute to many biologically significant processes. Here, we report first proteomic analysis generated from activated platelets. Using 1D SDS-PAGE liquid chromatography coupled a linear ion trap mass spectrometer, identification 578 proteins was accomplished using minimum 5 MS/MS detections at least two different peptides for each protein. These displayed intrinsic well-characterized on For...

The protein kinase B (AKT) pathway plays a key role in the regulation of cellular survival, apoptosis, and translation, has been shown to have prognostic significance number cancers. We sought define its extrahepatic cholangiocarcinoma.Two hundred twenty-one cholangiocarcinoma patients with clinicopathologic data, including were arrayed into tissue microarrays. Phosphorylated AKT (p-AKT), phosphorylated mammalian target rapamycin (p-mTOR), total phosphatase tensin homolog deleted on...

Foam cell formation from monocyte-derived macrophages is a hallmark of atherosclerotic lesions. Aspects this process can be recapitulated in vitro by exposing M-CSF-induced or platelet factor 4 (CXCL4)-induced to oxidized (ox) minimally modified (mm) low density lipoprotein (LDL). We measured gene expression peripheral blood mononuclear cells, monocytes, and treated with CXCL1 (GRO-alpha) CCL2 (MCP-1), as well foam cells induced native LDL, mmLDL, oxLDL using 22 Affymetrix chips. Using an...

Summary Microparticles (MPs) are small membrane vesicles generated by essentially all cell types. In the plasma, most MPs derived from platelets, but those other sources, particularly leukocytes (macrophages, lymphocytes, and neutrophils), endothelial cells, even smooth muscle cells can be detected appear to play an important role in normal physiology various diseases. previous work we analyzed proteome of isolated platelets (platelet MPs). Here, report on a comparative analysis...

Humans spend a significant portion of their daily life in cars. In this study, we investigate method to reduce motion sickness and allow people use virtual reality (VR) while riding As the arises primarily from sensory conflict between visual actual (or vestibular) motions, proposed approach attempts resolve mismatch by mixing visualizing estimated information motion, which is sensed on-board diagnostics inertial measurement unit modules attached vehicle. We conduct pilot experiment validate...

Some problems arise in analyzing massive complex data consisting of multivariate response variables and a large number multicollinear predictor variables, especially when the sample sizes compared to predictors are small. Rather than ordinary linear regression modeling approaches, latent variable approaches such as partial least squares can be used capture relationship between for cases. However, nonlinear relationships variable, performance inference prediction using deflated. Regression...

Tumor staging of extrahepatic bile duct (EBD) carcinoma is problematic for a number reasons, including definitional problems with the current T classification American Joint Committee on Cancer system and common occurrence severe desmoplastic stromal reaction around advancing edges these tumors. To address we evaluated depth invasion in 222 cases EBD by measuring distance between basal lamina adjacent normal epithelium to most deeply infiltrating tumor cells, compared this evaluation time...

Background and Aim We analyzed the characteristics of gastrointestinal neuroendocrine tumors examined outcomes safety modalities in rectal tumors. Methods Between 2007 2011, a total 91 patients with were retrospectively reviewed terms Results Sixty‐six had underwent endoscopic mucosal resection ( EMR , n = 29), submucosal dissection ESD 23), or transanal microsurgery TEM 14). The complete rate was higher group (82.7%) (100%) compared to (65.5%) P < 0.046). complication (47.8%) than...

Abstract The ultra‐wide bandgap and cost‐effective melt‐growth of β‐Ga 2 O 3 ensure its advantages over other wide materials, competitive electrical performance has been demonstrated in various device structures. In this paper, an asymmetric double‐gate (ADG) nanomembrane field‐effect transistor (FET) comprised a bottom‐gate (BG) metal‐oxide top‐gate (TG) metal‐semiconductor (MESFET) is demonstrated. Schottky contact properties are validated by characterizing the lateral barrier diode (SBD),...

Gene expression data can be associated with various clinical outcomes. In particular, these of importance in discovering survival-associated genes for medical applications. As alternatives to traditional statistical methods, sophisticated methods and software programs have been developed overcome the high-dimensional difficulty microarray data. Nevertheless, new algorithms are needed include practical functions such as discovery multiple sets incorporation risk factors, use R environment...

Abstract The choice of therapy for metastatic cancer is largely empirical because a lack chemosensitivity prediction available combination chemotherapeutic regimens. Here, we identify molecular models bladder carcinoma based on gene expression three widely used agents: cisplatin, paclitaxel, and gemcitabine. We measured the growth inhibition elicited by these agents in series 40 human urothelial cell lines correlated GI50 (50% inhibition) values with quantitative measures global to derive...

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is frequently inactivated in metastatic prostate cancer, yet the molecular consequences of this their association with phenotype are incompletely understood. We performed transcriptomic analysis identified genes altered by conditional PTEN reexpression C4-2, a human cancer cell line inactive PTEN. PTEN-regulated were disproportionately represented among progression metastasis but not those associated tumorigenesis. From former...

This paper develops a polynomial regression model with physical constraints to comprehensively analyze the complex motion of knuckleballs in soccer free-kick. The predictions match accurately ball trajectories extracted from recorded matches. uses function account for ball’s projectile-like motion. It further incorporates critical constraint that is related aerodynamic drag force on ball. implemented using relaxed iterative method. lsqlin solver MATLAB used each iteration. verified through...

The number of lymph nodes with metastases is known to be an important prognostic factor in carcinomas many organs. insufficient sampling has also been associated worse outcome several types carcinoma. However, the significance node dissection not well characterized extrahepatic bile duct (EBD) carcinomas. For 209 patients EBD carcinoma, total retrieved and metastatic were evaluated, other clinicopathologic variables correlated patient survival. was significantly survival this study. presence...

Abstract BACKGROUND Although the sixth edition of American Joint Committee on Cancer (AJCC) staging system for extrahepatic bile duct carcinoma was updated, has a problem T classification due to its ambiguous definition T1 as “tumor confined histologically” and T2 invading beyond duct.” METHODS The authors considered outermost part muscle layer or fibrous tissue within area starting from large clusters adipose duct. After designing precise wall, they analyzed new AJCC in 222 patients with...