A5071796522- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- ATP Synthase and ATPases Research
- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
Case Western Reserve University
2021-2024
Abstract Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as fluorescence signals. The powerful ability infer quantitative kinetic information from smFRET data is, however, complicated by experimental limitations. Diverse analysis tools have been developed overcome these hurdles but systematic comparison lacking. Here, we report results blind benchmark assessing eleven used rate constants...
ABSTRACT Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as fluorescence signals. The powerful ability infer quantitative kinetic information from smFRET data is, however, complicated by experimental limitations. Diverse analysis tools have been developed overcome these hurdles but systematic comparison lacking. Here, we report results blind benchmark assessing eleven used rate constants...