A5084562827- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Cell Image Analysis Techniques
- Nanoparticle-Based Drug Delivery
- Receptor Mechanisms and Signaling
- Force Microscopy Techniques and Applications
- Protein purification and stability
- Pharmacogenetics and Drug Metabolism
- ATP Synthase and ATPases Research
- Click Chemistry and Applications
- Advanced Polymer Synthesis and Characterization
- Advanced Electron Microscopy Techniques and Applications
- Alzheimer's disease research and treatments
- Protein Interaction Studies and Fluorescence Analysis
- Advanced Biosensing Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Photoreceptor and optogenetics research
Novo Nordisk Foundation
2019-2025
University of Copenhagen
2016-2025
Novo Nordisk (Denmark)
2021-2025
Nano Hydrophobics (United States)
2024
Novo Nordisk (Finland)
2024
Cellular Dynamics International (United States)
2024
Novo Nordisk (United Kingdom)
2023
Lundbeck Foundation
2009-2020
Frederiksberg Hospital
2019
University College Copenhagen
2011
In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within central water pool block-copolymer membrane polymersomes. A one-pot multistep reaction sequence is performed with nanoreactor in combination free Candida antarctica lipase B (CALB) bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)).
Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling intermediates and prevent undesired metabolic cross-talk while maintaining flexibility. Here we report isolation dynamic metabolon that catalyzes formation cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The was reconstituted liposomes, which demonstrated importance membrane surface charge presence...
Abstract Transmembrane nanostructures like ion channels and transporters perform key biological functions by controlling flow of molecules across lipid bilayers. Much work has gone into engineering artificial nanopores applications in selective gating molecules, label-free detection/sensing biomolecules DNA sequencing have shown promise. Here, we use origami to create a synthetic 9 nm wide nanopore, controlled programmable, lipidated flaps equipped with size-selective system for the...
A simple and versatile method for the preparation of functional enzyme−gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase attached to azide-functionalized water-soluble gold nanoparticles under retention enzymatic activity. The products have characterized by gel electrophoresis fluorometric activity assay. It is estimated that equivalent approximately seven...
Biohybrid amphiphiles have been prepared from terminal azide functionalised polystyrene and an alkyne peptide or protein via a Cu(I) catalysed Huisgen [3 + 2] dipolar cycloaddition reaction.
Liposomes, or vesicles, have been studied extensively both as models of biological membranes and drug delivery vehicles. Typically it is assumed that all liposomes within the same preparation are identical. Here by employing pairs fluorescently labeled lipids we demonstrated an up to 10-fold variation in relative lipid composition individual with diameters between 50 nm 15 μm. Since physicochemical properties directly linked their composition, a direct consequence compositional...
Abstract Surface engineering of nanomaterials allows fine tuning their interactions with biological systems, and thus can benefit applications in monitoring intracellular events. Herein, the facile synthesis ligand‐functionalized gold nanoclusters (AuNCs) as probes targeting highly reactive oxygen species (hROS, such •OH, ClO − , ONOO ) is demonstrated. Selected ligands quaternary ammonium oligopeptides are utilized to modulate surface chemistry AuNCs. It shown that AuNCs decorated...
In eukaryotes, P-type adenosine triphosphatases (ATPases) generate the plasma membrane potential and drive secondary transport systems; however, despite their importance, regulation remains poorly understood. We monitored at single-molecule level activity of prototypic proton-pumping ATPase Arabidopsis thaliana isoform 2 (AHA2). Our measurements, combined with a physical nonequilibrium model vesicle acidification, revealed that pumping is stochastically interrupted by long-lived (~100...
Single-particle tracking (SPT) is a key tool for quantitative analysis of dynamic biological processes and has provided unprecedented insights into wide range systems such as receptor localization, enzyme propulsion, bacteria motility, drug nanocarrier delivery. The inherently complex diffusion in can vary drastically both time across systems, consequently imposing considerable analytical challenges, currently requires an priori knowledge the system. Here we introduce method SPT data...
Abstract Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as fluorescence signals. The powerful ability infer quantitative kinetic information from smFRET data is, however, complicated by experimental limitations. Diverse analysis tools have been developed overcome these hurdles but systematic comparison lacking. Here, we report results blind benchmark assessing eleven used rate constants...
The morphology of protein assemblies impacts their behaviour and contributes to beneficial aberrant cellular responses. While single-molecule localization microscopy provides the required spatial resolution investigate these assemblies, lack universal robust analytical tools extract quantify underlying structures limits this powerful technique. Here we present SEMORE, a semi-automatic machine learning framework for universal, system- input-dependent, analysis super-resolution data. SEMORE...
Gut positioniert: Durch gezielte Positionierung von Glucoseoxidase (GOX) und Meerrettichperoxidase (HRP) im zentralen Wasserreservoir bzw. in der Blockcopolymermembran Polymersomen lassen sich Nanoreaktoren konstruieren. Mit ihnen kann Kombination mit Candida-antarctica-Lipase B (CALB) Lösung eine mehrstufige Eintopfreaktionssequenz durchgeführt werden (siehe Bild; ABTS: 2,2′-Azinobis(3-ethylbenzothiazolin-6-sulfonsäure)). Supporting information for this article is available on the WWW under...
Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because the pathological environment lungs. Here, we present an "environment-adaptive" nanoparticle composed a solid poly lactic-co-glycolic acid (PLGA) core and mucus-inert, enzymatically cleavable shell d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for site-specific delivery antibiotics to biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were...
Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development high throughput methodologies growth commercial instrumentation have outpaced rapid, standardized, automated to objectively analyze wealth produced data. Here we present DeepFRET, automated, open-source standalone solution based on deep learning, where only crucial human intervention in transiting from raw microscope images histograms...
Metabolic control is mediated by the dynamic assemblies and function of multiple redox enzymes. A key element in these assemblies, P450 oxidoreductase (POR), donates electrons selectively activates numerous (>50 humans >300 plants) cytochromes (CYPs) controlling metabolism drugs, steroids xenobiotics natural product biosynthesis plants. The mechanisms underlying POR-mediated CYP remain poorly understood to date no ligand binding has been described regulate specificity POR. Here, using a...
Endocytosis, required for the uptake of receptors and their ligands, can also introduce pathological aggregates such as α-synuclein (α-syn) in Parkinson’s Disease. We show here unexpected presence intrinsically perforated endolysosomes neurons, suggesting involvement genesis toxic α-syn induced by internalized preformed fibrils (PFFs). Aggregation endogenous late endosomes lysosomes human iPSC-derived neurons (iNs), seeded PFFs, caused death iNs but not parental iPSCs non-neuronal cells....
A giant amphiphile consisting of polystyrene end-capped with permethylated beta-cyclodextrin was synthesised and found to form vesicular structures when injected as a solution in THF into water. The ability the cyclodextrins on surface polymersomes inclusion complexes hydrophobic compounds tested by carrying out competition experiment fluorescent probe sensitive polarity surrounding medium. It that 1-adamantol can displace from cavities cyclodextrin moieties polymersomes. recognition...
A confocal fluorescence microscopy-based assay was used for studying the influence of preparation route on supramolecular organization lipids in a vesicular system. In this work, vesicles composed cholesterol and CTAB (1/1 mol %) or DOPC (2/8 incorporating two membrane dyes were prepared by either compressed fluid (CF)-based method (DELOS-susp) conventional film hydration procedure. They subsequently immobilized imaged individually using microscope. Two integrated intensities, I(dye1)...