A5004634931- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Hydrogels: synthesis, properties, applications
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Conducting polymers and applications
- Extracellular vesicles in disease
- SARS-CoV-2 detection and testing
- Bioinformatics and Genomic Networks
- Advanced Biosensing Techniques and Applications
- Protein Structure and Dynamics
- MicroRNA in disease regulation
- Neurotransmitter Receptor Influence on Behavior
- Parkinson's Disease Mechanisms and Treatments
- Integrated Circuits and Semiconductor Failure Analysis
Aarhus University
2023-2025
University of Copenhagen
2019-2022
Novo Nordisk Foundation
2021
University of Southern Denmark
2021
Abstract Transmembrane nanostructures like ion channels and transporters perform key biological functions by controlling flow of molecules across lipid bilayers. Much work has gone into engineering artificial nanopores applications in selective gating molecules, label-free detection/sensing biomolecules DNA sequencing have shown promise. Here, we use origami to create a synthetic 9 nm wide nanopore, controlled programmable, lipidated flaps equipped with size-selective system for the...
Synthetic nanoparticles as lipid (LNPs) are widely used drug delivery vesicles. However, they hold several drawbacks, including low biocompatibility and unfavorable immune responses. Naturally occurring extracellular vesicles (EVs) the potential native, safe, multifunctional nanovesicle carriers. loading of EVs with large biomolecules remains a challenge. Here, we present controlled methodology using DNA-mediated programmed fusion between messenger RNA (mRNA)-loaded liposomes. The efficiency...
Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development high throughput methodologies growth commercial instrumentation have outpaced rapid, standardized, automated to objectively analyze wealth produced data. Here we present DeepFRET, automated, open-source standalone solution based on deep learning, where only crucial human intervention in transiting from raw microscope images histograms...
Abstract The dopamine transporter facilitates reuptake from the extracellular space to terminate neurotransmission. belongs neurotransmitter:sodium symporter family, which includes transporters for serotonin, norepinephrine, and GABA that utilize Na + gradient drive uptake of substrate. Decades ago, it was shown serotonin also antiports K , but investigations -coupled transport in other symporters have been inconclusive. Here, we show ligand binding Drosophila - human are inhibited by...
DNA nanopores have emerged as powerful tools for molecular sensing, but the efficient insertion of large into lipid membranes remains challenging. In this study, we investigate potential cell-penetrating peptides (CPPs), specifically SynB1 and GALA, to enhance efficiency nanopores. We constructed SynB1- or GALA-functionalized with an 11 nm inner diameter visualized quantified their membrane using a TIRF microscopy-based single-liposome assay. The results demonstrated that incorporating...
Hydrogel biomaterials have been extensively explored for applications in medicine, materials science, and the development of functionalized materials. Traditionally, hydrogels were produced using simple polymers, but advancements over recent decades enabled use biological such as proteins, peptides, polysaccharides, even amyloid fibrils. Among these, amyloid-based demonstrated unique advantages, including enhanced cell adhesion differentiation. Furthermore, they can be engineered living...
Neurodegenerative disorders like Parkinson's disease (PD) pose significant health challenges. A major hallmark is the aggregation of α-synuclein into toxic oligomers (αSO) and fibrils. While many efforts focus on slowing progression, molecular origins mechanisms αSO toxicity remain poorly understood, particularly its proposed link to membrane disruption. To address this, we have developed a single-vesicle analysis platform for direct real-time measurements interaction. This allows us show...
Abstract Understanding the importance of ligand patterning in biological processes requires precise control over molecular positioning and spacing. While DNA origami structures offer nanoscale precision biomolecule arrangement, their applications are limited by challenges related to structural stability, scalability, surface area. Here, we present a straightforward rapid stamping technique for transferring oligonucleotide patterns onto surfaces, visualized using DNA-PAINT super-resolution...
Abstract Functional amyloids, beneficial to the organism producing them, are found throughout life, from bacteria humans. While disease‐related amyloids form by uncontrolled aggregation, fibrillation of functional amyloid is regulated complex cellular machinery and optimized sequences, including so‐called gatekeeper residues such as Asp. However, molecular basis for this regulation remains unclear. Here we investigate how introduction additional affects fibril formation stability in CsgA E....
Abstract Liposomes are versatile three‐dimensional, biomaterial‐based frameworks that can spatially enclose a variety of organic and inorganic biomaterials for advanced targeted‐delivery applications. Implementation external‐stimuli‐controlled release their cargo will significantly augment wide application liposomal drug delivery. This paper presents the synthesis carbohydrate‐derived lipid, capable changing its conformation depending on presence Zn 2+ : an active state in ions back to...
Abstract Single molecule Förster Resonance energy transfer (smFRET) is a mature and adaptable method for studying the structure of biomolecules integrating their dynamics into structural biology. The development high throughput methodologies growth commercial instrumentation have outpaced rapid, standardized, fully automated to objectively analyze wealth produced data. Here we present DeepFRET, an standalone solution based on deep learning, where only crucial human intervention in transiting...
Abstract Combinatorial high throughput methodologies are central for both screening and discovery in synthetic biochemistry biomedical sciences. They are, however, often reliant on large scale analyses thus limited by long running time excessive materials cost. We herein present S ingle PAR ticle C ombinatorial multiplexed L iposome fusion mediated D NA (SPARCLD), the parallelized, multi-step non-deterministic of individual zeptoliter nanocontainers. observed directly efficient (>93%),...
Abstract Combinatorial high throughput methodologies are central for both screening and discovery in synthetic biochemistry biomedical sciences. They are, however, often reliant on large scale analyses thus limited by long running time excessive materials cost. We herein present Single PARticle multiplexed Liposome fusion mediated DNA (SPARCLD), the parallelized, multi-step non-deterministic of individual zeptoliter nanocontainers. observed directly efficient (>93%), leakage free...
Stimuli-responsive lipids based on a glucose head group could promote pore formation in the membrane of liposomes upon treatment with EDTA. Using total internal reflection fluorescence (TIRF) microscopy setup, it was possible to monitor release fluorescent dye from containing metal chelator-responsive lipids. The remained stable and able incapsulate cargo absence Only when introducing chelating EDTA would they their through pores formed membrane. This serve as platform for future...
Abstract The dopamine transporter (DAT) facilitates reuptake from the extracellular space, and thereby terminates neurotransmission refills cellular stores of dopamine. DAT belongs to neurotransmitter:sodium symporter (NSS) family, which includes similar transporters for serotonin, norepinephrine, GABA. A hallmark NSS proteins is their ability utilize energy stored in inward-directed Na+ gradient drive uphill transport substrate. Decades ago, it was shown that serotonin also...