A5059976898- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Protein Structure and Dynamics
- Protist diversity and phylogeny
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Cannabis and Cannabinoid Research
- Escherichia coli research studies
- Biochemical and Molecular Research
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Advanced Electron Microscopy Techniques and Applications
- Metal Extraction and Bioleaching
- Electrochemical sensors and biosensors
- RNA modifications and cancer
- Pharmacological Receptor Mechanisms and Effects
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
Aarhus University
2016-2025
Cable bacteria of the family Desulfobulbaceae form centimeter-long filaments comprising thousands cells. They occur worldwide in surface aquatic sediments, where they connect sulfide oxidation with oxygen or nitrate reduction via long-distance electron transport. In absence pure cultures, we used single-filament genomics and metagenomics to retrieve draft genomes 3 marine Candidatus Electrothrix 1 freshwater Ca. Electronema species. These contain >50% unknown genes but still share their core...
Cable bacteria are long, multicellular filaments that can conduct electric currents over centimeter-scale distances. All cable identified to date belong the deltaproteobacterial family Desulfobulbaceae and have not been isolated in pure culture yet. Their taxonomic delineation exact phylogeny is uncertain, as most studies so far reported only short partial 16S rRNA sequences or relied on identification by a combination of filament morphology rRNA-targeted fluorescence situ hybridization with...
The bacterial relBE locus encodes a toxin-antitoxin complex in which the toxin, RelE, is capable of cleaving mRNA ribosomal A site cotranslationally. antitoxin, RelB, both binds and inhibits regulates transcription through operator binding conditional cooperativity controlled by RelE. Here, we present crystal structure intact Escherichia coli RelB2E2 at 2.8 Å resolution, comprising RelB-inhibited RelE RelB dimerization domain that DNA. associate into V-shaped heterotetrameric with...
Toxin–antitoxin (TA) loci are common in archaea and prokaryotes allow cells to rapidly adapt changing environmental conditions through release of active regulators metabolism. Many toxins endonucleases that target cellular mRNA tRNAs, while the antitoxins tightly wrap around inhibit them under normal circumstances. The also bind operators promoter regions cognate TA operon thereby regulate transcription. For enteric vapBC loci, VapC specifically cleave tRNAfMet thus down-regulate protein...
Cable bacteria are long, multicellular micro-organisms that capable of transporting electrons from cell to along the longitudinal axis their centimeter-long filaments. The conductive structures mediate this long-distance electron transport thought be located in envelope. Therefore, study examines detail architecture envelope cable bacterium filaments through a combination classical microscopy, cryo-based microscopy and tomography, focused ion beam-scanning atomic force microscopy. We...
The Sm and Sm-like proteins are conserved in all three domains of life have emerged as important players many different RNA-processing reactions. Their proposed role is to mediate RNA–RNA and/or RNA–protein interactions. In marked contrast eukaryotes, bacteria appear contain only one distinct protein belonging the Hfq family proteins. Similarly, there generally or two subtypes Sm-related archaea, but at least archaeon, Methanococcus jannaschii , encodes a that related Hfq. This archaeon does...
Cable bacteria are multicellular filamentous within the Desulfobulbaceae that couple oxidation of sulfide to reduction oxygen over centimeter distances via long distance electron transport (LDET). So far, none freshwater or marine cable species have been isolated into pure culture. Here we describe a method for establishing stable single-strain bacterium culture in partially sterilized sediment. By repeated transfers single filament from pond sediment autoclaved sediment, obtained strain GS,...
Hfq proteins are common in many species of enterobacteria, where they participate RNA folding and translational regulation through pairing small RNAs messenger RNAs. share the distinctive Sm fold, form ring-shaped structures similar to those Sm/Lsm regulating mRNA turnover eukaryotes. However, bacterial homohexameric, whereas eukaryotic heteroheptameric. Recently, with poor sequence conservation were identified archaea cyanobacteria. In this article, we describe crystal from cyanobacteria...
Abstract Cable bacteria are centimeter-long multicellular conducting electricity through periplasmic conductive fibers (PCFs). Using single-strain enrichments of the genera Electrothrix and Electronema we systematically investigate variations similarities in morphology electrical properties across both genera. Electrical conductivity different PCFs spans three orders magnitude warranting further investigations plasticity their conduction machinery. electron microscopy elemental analyses,...
Abstract Tetrahydrocannabinol (THC) is the principal psychoactive compound derived from cannabis plant Cannabis sativa and approved for emetic conditions, appetite stimulation sleep apnea relief. THC’s actions are mediated primarily by cannabinoid receptor CB 1 . Here, we determine cryo-EM structure of HU210, a THC analog widely used tool compound, bound to its primary transducer, G i1 We leverage this docking 1000 ns molecular dynamics simulations 10 structural analogs delineating their...
Sub2p/UAP56 is a highly conserved DEAD-box RNA helicase involved in the packaging and nuclear export of mRNA/protein particles (mRNPs). In Saccharomyces cerevisiae , Sub2p recruited to active chromatin by pentameric THO complex incorporated into larger transcription–export (TREX) complex. also plays role maintenance genome integrity as its inactivation causes severe transcription-dependent recombination DNA. Despite central early mRNP biology, little known about function. Here, we report...
Type IV pili are widely expressed among Gram-negative bacteria, where they involved in biofilm formation, serve the transfer of DNA, motility and bacterial attachment to various surfaces. Shewanella oneidensis also supposed play an important role extracellular electron by sediments containing acceptors potentially forming conductive nanowires.The potential nanowire type pilin PilBac1 from S. was characterized a combination complementary structural methods atomic structure determined at...
Abstract Cable bacteria encompass at least two genera, and they are known to vary greatly in habitat preferences filament thickness. We systematically investigated variations similarities cellular structures electrical properties of different cable strains. Using SEM, TEM, STEM-EDX ToF-SIMS, we characterized shared features bacteria, such as inner outer membranes, surface layer cell junction architecture, well strain specific features, like the number size periplasmic conductive fibers...
Exploiting the anomalous signal of intrinsic S atoms to phase a protein structure is advantageous, as ideally only single well diffracting native crystal required. However, sulfur weak scatterer at typical wavelengths used for X-ray diffraction experiments, and therefore SAD data sets need be recorded with high multiplicity. In this study, small pilin was determined by despite several obstacles such low (a theoretical Bijvoet ratio 0.9% wavelength 1.8 Å), radiation damage-induced reduction...
Upon release from the stable complex formed with its antitoxin VapB, toxin VapC (MvpT) of Gram-negative pathogen Shigella flexneri is capable globally down-regulating translation by specifically cleaving initiator tRNA(fMet) in anticodon region. Recombinant VapC(D7A) harbouring an active-site mutation was overexpressed Escherichia coli, purified to homogeneity and crystallized vapour-diffusion technique. A preliminary X-ray crystallographic analysis shows that crystals diffracted at least...
<title>Abstract</title> Δ<sup>9</sup>-tetrahydrocannabinol (THC) is the principal psychoactive compound derived from cannabis plant Cannabis sativa and approved for emetic conditions, appetite stimulation sleep apnea relief. THC’s actions are mediated primarily by cannabinoid receptor CB<sub>1</sub>. Here, we determine cryo-EM structure of HU210, a THC analog widely used tool compound, bound to CB<sub>1</sub> its primary transducer, G<sub>i1</sub>. We leverage this docking 1,000 ns molecular...
The advantage of using the anomalous signal sulfur for phase determination is that only a single, well-diffracting crystal needed and native structure will be obtained. Using long-wavelength S-SAD to resolution 1.9 Å we have determined novel an 89 residue protein with 2 Cysteines fixed in disulfide bridge. To best our knowledge, Bijvoet ratio example one smallest which successful solution by has been reported. Data were collected on 3 different volumes single at beamline 14.1. BESSY II,...