A5063634428- Advanced NMR Techniques and Applications
- Solid-state spectroscopy and crystallography
- RNA Research and Splicing
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- Advanced MRI Techniques and Applications
- Glycosylation and Glycoproteins Research
- Advanced Neuroimaging Techniques and Applications
- Chemical Synthesis and Analysis
- NMR spectroscopy and applications
- RNA and protein synthesis mechanisms
- Electron Spin Resonance Studies
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Oral microbiology and periodontitis research
- Endoplasmic Reticulum Stress and Disease
- Multiple Sclerosis Research Studies
- Bacterial biofilms and quorum sensing
- Microtubule and mitosis dynamics
- Supramolecular Self-Assembly in Materials
- Alkaline Phosphatase Research Studies
- Molecular Biology Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- DNA and Nucleic Acid Chemistry
Leibniz University Hannover
2019-2020
Suez University
2010-2020
Helmholtz Centre for Infection Research
2015-2018
Leibniz University of Applied Sciences
2018
University of Guelph
2006-2015
Suez Canal University
2010
Massey University
2010
Solid-state NMR (ssNMR) is applicable to high molecular-weight (MW) protein assemblies in a non-amorphous precipitate. The technique yields atomic resolution structural information on both soluble and insoluble particles without limitations of MW or requirement crystals. Herein, we propose demonstrate an approach that the structure protein-RNA complexes (RNP) solely from ssNMR data. Instead using low-sensitivity magnetization transfer steps between heteronuclei RNA, measure paramagnetic...
The 18.5 kDa myelin basic protein (MBP), the most abundant splice isoform in adult mammalian myelin, is a multifunctional, intrinsically disordered involved development and compaction of sheath central nervous system. A highly conserved segment comprises membrane-anchoring amphipathic α-helix followed by proline-rich that represents ligand for SH3 domain-containing proteins. Here, we have determined using solution nuclear magnetic resonance spectroscopy structure 36-residue peptide fragment...
Abstract Curli are functional bacterial amyloids produced by an intricate biogenesis machinery. Insights into their folding and regulation can advance our understanding of amyloidogenesis. However, gaining detailed structural information amyloids, tendency for polymorphisms, remains challenging. Herein we compare high‐quality solid‐state NMR spectra from biofilm‐derived recombinantly curli provide evidence that they adopt a similar, well‐defined β‐solenoid arrangement. subunits consist five...
Significance The mitochondrial antiviral signaling protein (MAVS) belongs to the emerging class of higher-order machines that adopt a filamentous state on activation and propagate in prion-like manner. Structures helical filaments are challenging due their size variable symmetry parameters, which notoriously difficult obtain, but prerequisite for structure determination by electron microscopy solid-state NMR. Here we describe strategy efficient de novo grid-search approach based exclusively...
The 18.5 kDa myelin basic protein (MBP), the most abundant splice isoform in human adult myelin, is a multifunctional, intrinsically disordered that maintains compact assembly of sheath central nervous system. Protein deimination and phosphorylation are two key posttranslational modifications whose balance determines local microdomain stability function. It has previously been shown MBP solution causes both polymerization G-actin to F-actin bundling microfilaments, binds them negatively...
The genes of the oligodendrocyte lineage (Golli) encode a family developmentally regulated isoforms myelin basic protein. "classic" MBP arise from transcription start site 3, whereas Golli-specific 1, and comprise both classic sequences. Golli isoform BG21 has been suggested to play roles in myelination T cell activation pathways. It is an intrinsically disordered protein, thereby presenting large effective surface area for interaction with other proteins such as Golli-interacting We have...
Abstract Solid‐state NMR (ssNMR) is applicable to high molecular‐weight (MW) protein assemblies in a non‐amorphous precipitate. The technique yields atomic resolution structural information on both soluble and insoluble particles without limitations of MW or requirement crystals. Herein, we propose demonstrate an approach that the structure protein–RNA complexes (RNP) solely from ssNMR data. Instead using low‐sensitivity magnetization transfer steps between heteronuclei RNA, measure...
Abstract A recombinant form of the murine Golli‐myelin basic protein (MBP) isoform BG21 (rmBG21) has been expressed in E. coli , and isolated to 96% purity via metal chelation chromatography. Characteristic yields were 6–8 mg per liter culture either minimal M9 or standard Luria‐Bertani media. Circular dichroism spectroscopy showed that rmBG21 had a large proportion random coil aqueous solution, but gained α‐helix presence monosialoganglioside G M1 PI(4)P, as well membrane‐mimetic solvent...
Abstract Curli sind funktionelle bakterielle Amyloide, die durch eine ausgefeilte Biogenesemaschinerie hergestellt werden. Einblicke in ihre Faltung und Regulierung kann unser Verständnis der Amyloidbildung erweitern. Allerdings ist Aufklärung hochaufgelöster Strukturen von Amyloiden aufgrund ihrer Tendenz zu strukturellen Polymorphismen weiterhin Herausforderung. Hier vergleichen wir qualitativ hochwertige Festkörper‐NMR‐Spektren Curli, aus Biofilmen isoliert wurden, mit rekombinant...