A5068897692- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Sepsis Diagnosis and Treatment
- Congenital heart defects research
- Hemodynamic Monitoring and Therapy
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Mitochondrial Function and Pathology
- Cardiac Arrest and Resuscitation
- Trauma and Emergency Care Studies
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Air Quality and Health Impacts
- Platelet Disorders and Treatments
- Inflammatory Biomarkers in Disease Prognosis
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Response and Inflammation
- Cell death mechanisms and regulation
- Psoriasis: Treatment and Pathogenesis
- Thermal Regulation in Medicine
- Pancreatic function and diabetes
- Biotechnology and Related Fields
Taipei Medical University
2018-2025
Taipei Medical University Hospital
2022-2025
Institute of Biomedical Sciences, Academia Sinica
2015-2018
Sky Ridge Medical Center
2018
Academia Sinica
2015-2017
Fudan University
2017
Massachusetts Institute of Technology
2017
Harvard University
2017
The First Affiliated Hospital, Sun Yat-sen University
2017
Zhongshan Hospital
2017
Extracellular vesicles (EVs) from cultured cells or bodily fluids have been demonstrated to show therapeutic value following myocardial infarction. However, challenges in donor variation, EV generation and isolation methods, material availability hindered their use. Here, we that human clinical-grade platelet concentrates a blood establishment can be used rapidly generate high concentrations of purity EVs sero-converted lysate (SCPL-EVs) with minimal processing, using size-exclusion...
Near-infrared spectroscopy (NIRS) noninvasively measures peripheral tissue oxygen saturation (StO2). NIRS may be utilized along with a vascular occlusion test, in which limb blood flow is temporarily occluded and released, to quantify bed's rate of exchange during ischemia recovery. The objective this study was test the hypothesis that NIRS-derived StO2 (StO2 initial, recovery) identify patients who are shock at increased risk organ dysfunction (Sequential Organ Failure Assessment (SOFA)...
The severe acute respiratory syndrome coronavirus (SARS-CoV)-2 disease (COVID)-19 is having profound effects on the global economy and food trade. Limited data are available how this pandemic affecting our dietary lifestyle-related behaviors at level. Google Trends was used to obtain worldwide relative search volumes (RSVs) covering a timeframe from before COVID-19 1 June 2019 27 April 2020. Spearman’s rank-order correlation coefficients were measure relationships between daily confirmed...
Abstract Nanoparticles represent an attractive option for systemic delivery of therapeutic compounds to the heart following myocardial infarction. However, it is well known that physicochemical properties nanoparticles such as size, shape and surface modifications can vastly alter distribution uptake injected nanoparticles. Therefore, we aimed provide examination rapid size-dependent fluorescent PEG-modified polystyrene administered immediately cardiac ischaemia-reperfusion injury in mice....
The gut microbiome and its metabolites are increasingly implicated in several cardiovascular diseases, but their role human myocardial infarction (MI) injury responses have yet to be established. To address this, we examined stool samples from 77 ST-elevation MI (STEMI) patients using 16 S V3-V4 next-generation sequencing, metagenomics machine learning. Our analysis identified an enriched population of butyrate-producing bacteria. These findings were then validated a controlled...
Pluripotent-cell-derived cardiomyocytes have great potential for use in research and medicine, but limitations their maturity currently constrain usefulness. Here, we report a method improving features of maturation murine human embryonic-stem-cell-derived (m/hESC-CMs). We found that coculturing m/hESC-CMs with endothelial cells improves upregulates several microRNAs. Delivering four these microRNAs, miR-125b-5p, miR-199a-5p, miR-221, miR-222 (miR-combo), to resulted improved sarcomere...
This study comprehensively investigates the changing biodistribution of fluorescent-labelled polystyrene latex bead nanoparticles in a mouse model inflammation.
The blood-brain barrier (BBB) selectively controls the passage of endogenous and exogenous molecules between systemic circulation brain parenchyma. Nanocarrier-based drugs such as liposomes nanoparticles are an attractive prospect for cancer therapy since they can carry a drug payload be modified to improve targeting retention at desired site. However, BBB prevents most therapeutic from entering brain, including physically restricting nanoparticles. In this paper, we show that low dose...
Cardiac regeneration after injury is limited by the low proliferative capacity of adult mammalian cardiomyocytes (CMs). However, certain animals readily regenerate lost myocardium through a process involving dedifferentiation, which unlocks their capacities.
Research Article23 December 2016Open Access Transparent process Reprogramming-derived gene cocktail increases cardiomyocyte proliferation for heart regeneration Yuan-Yuan Cheng Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan Biomedical Academia Sinica, Search more papers by this author Yu-Ting Yan David J Lundy Annie HA Lo Yu-Ping Wang Shu-Chian Ruan Po-Ju Lin Patrick CH Hsieh Corresponding Author [email protected] orcid.org/0000-0002-8910-3596 Department...
Extracellular vesicles (EVs) hold significant promise as biomarkers and therapeutics, yet their isolation remains challenging due to low abundance complex sample matrices. Here, we introduce EV-Osmoprocessor (EVOs), a novel device that leverages osmosis-driven filtration for rapid efficient EV isolation. EVOs employs high osmolarity polymer solution concentrate EVs while simultaneously removing smaller contaminants. Compared traditional methods such ultracentrifugation precipitation, offers...
Abstract Extracellular vesicles (EVs), lipid bilayer‐bound particles secreted by cells, have attracted significant research attention for their roles in aging‐related disorders, including cardiovascular disease, metabolic dysfunction, cancer, and neurodegeneration. Research shows that EV cargo function are influenced factors age, disease state, exercise, nutrition sleep, they may modulate various aging‐associated processes such as stem cell renewal, nutrient sensing, senescence,...
This work describes protocols for preparing specific forms of human platelet lysates from pooled concentrates (PCs) and the isolation platelet-derived extracellular vesicles (p-EVs). Clinical-grade PCs can be sourced blood establishments immediately following expiration transfusion use. Here, we describe methods to process into which p-EVs isolated. Each lysate type is prepared using activation processing produce distinct products that may useful in different applications. For example,...
A reloadable drug capture system facilitates multidrug therapy for neovascularization after limb ischemia.
Abstract Calorie restriction (CR) and fasting affect lifespan, disease susceptibility response to acute injury across multiple animal models, including ischaemic injuries such as myocardial infarction or kidney hypoxia. The cargo function of circulating extracellular vesicles (EV) respond changes in host physiology, exercise, injury, other interventions. Thus, we hypothesised that EVs induced following CR may reflect some the beneficial properties itself. In a pilot study, were isolated from...
Rationale: Reducing cardiomyocyte death and enhancing their proliferation after myocardial infarction is perhaps the single largest challenge for cardiac tissue regeneration. Survivin (SVV) smallest member of inhibitor apoptosis (IAP) family but plays two important roles; inhibiting caspase-9 activation in intrinsic pathway, regulating microtubule dynamics chromosome segregation during cell division. Genetic depletion SVV leads to incomplete division abnormal heart development. However,...