A5073582174- Reproductive Biology and Fertility
- Hippo pathway signaling and YAP/TAZ
- Kruppel-like factors research
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Fibroblast Growth Factor Research
- Reproductive System and Pregnancy
- Renal and related cancers
- Ovarian cancer diagnosis and treatment
- Uterine Myomas and Treatments
- Virus-based gene therapy research
- Gynecological conditions and treatments
- Sperm and Testicular Function
Massachusetts General Hospital
2023-2025
Harvard University
2023-2025
Université de Montréal
2017-2024
Physiologie de la Reproduction et des Comportements
2024
Abstract Eighty percent of the estimated 600 million domestic cats in world are free-roaming. These typically experience suboptimal welfare and inflict high levels predation on wildlife. Additionally, euthanasia healthy animals overpopulated shelters raises ethical considerations. While surgical sterilization is mainstay pet population control, there a need for efficient, safe, cost-effective permanent contraception alternatives. Herein, we report evidence that single intramuscular treatment...
Anti-Müllerian hormone (AMH) protects the ovarian reserve from chemotherapy, and this effect is most pronounced with Doxorubicin (DOX). However, DOX toxicity AMH rescue mechanisms in ovary have remained unclear. Herein, we characterize consequences of these treatments cell types using scRNAseq. DOX-induced DNA damage activates Tp53 class mediators across types. In mesenchyme, cotreatment halts theca progenitor differentiation reduces apoptotic gene expression. preantral granulosa cells,...
Abstract Background The LH surge is a pivotal event that triggers multiple key ovarian processes including oocyte maturation, cumulus expansion, follicular wall rupture and luteinization of mural granulosa theca cells. Recently, LH-dependent activation the Hippo signaling pathway has been shown to be required for differentiation cells into luteal Still, precise interactions between in murine remain elucidated. Methods To detect expression effectors pathway, western blot, immunohistochemical...
Wnt4 and Wnt5a have well-established roles in the embryonic development of female reproductive tract, as well implantation, decidualization, ovarian function adult mice. Although these appear to overlap, whether are functionally redundant tissues has not been determined. We addressed this by concomitantly inactivating Müllerian mesenchyme granulosa cells crossing mice bearing floxed alleles Amhr2cre strain. Whereas fertility was reduced ∼50% Wnt4flox/flox; Amhr2cre/+ Wnt5aflox/flox; females,...
WNT signaling regulates a variety of ovarian processes, including follicle development, granulosa cell (GC) proliferation and differentiation, steroidogenesis, ovulation. The secreted frizzled-related proteins (SFRPs) comprise family antagonists. Sfrp4 expression was previously reported to be induced in GCs cumulus cells vivo following human chorionic gonadotropin treatment, suggesting that it may play key roles expansion, ovulation/luteinization, corpus luteum (CL) function. In this study,...
The development of the Müllerian ducts into female reproductive tract requires coordination multiple signaling pathways that regulate proliferation, apoptosis and differentiation. Hippo pathway has been reported to interact with several established roles in duct development; yet, its potential function remain mostly uncharacterized. objective this study was therefore characterize transcriptional coactivators YAP TAZ using transgenic mouse models. This report shows concomitant conditional...
Abstract Previous in vitro studies have suggested that SLIT ligands could play roles regulating ovarian granulosa cell proliferation and gene expression, as well luteolysis. However, no vivo study of Slit function has been conducted to date. Here, we investigated the potential role Slit1 biology using a Slit1-null mouse model. Female mice were found produce larger litters than their wild-type counterparts due increased ovulation rates. Increased weights animals be presence greater numbers...