A5043041852- Reproductive Biology and Fertility
- Ovarian cancer diagnosis and treatment
- Reproductive System and Pregnancy
- Renal and related cancers
- Sperm and Testicular Function
- Single-cell and spatial transcriptomics
- Ovarian function and disorders
- Endometriosis Research and Treatment
- Virus-based gene therapy research
Massachusetts General Hospital
2022-2025
Harvard University
2022-2025
The estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate hormonal ovulatory functions ovary. Folliculogenesis follicle progression require orchestrated response a variety cell types to allow maturation its sequela, ovulation, corpus luteum formation, wound repair. Little known about state dynamics ovary during paracrine factors that help this process. Herein, we used single-cell RNA sequencing evaluate transcriptome...
Abstract Eighty percent of the estimated 600 million domestic cats in world are free-roaming. These typically experience suboptimal welfare and inflict high levels predation on wildlife. Additionally, euthanasia healthy animals overpopulated shelters raises ethical considerations. While surgical sterilization is mainstay pet population control, there a need for efficient, safe, cost-effective permanent contraception alternatives. Herein, we report evidence that single intramuscular treatment...
Anti-Müllerian hormone (AMH) protects the ovarian reserve from chemotherapy, and this effect is most pronounced with Doxorubicin (DOX). However, DOX toxicity AMH rescue mechanisms in ovary have remained unclear. Herein, we characterize consequences of these treatments cell types using scRNAseq. DOX-induced DNA damage activates Tp53 class mediators across types. In mesenchyme, cotreatment halts theca progenitor differentiation reduces apoptotic gene expression. preantral granulosa cells,...
Significance This study aims to identify drugs that activate the Mullerian inhibiting substance pathway be used for contraception or other applications in women’s health. We describe a high-throughput screening pipeline small molecules type 2 receptor (MISR2) and validate their activity bioassays. five compounds from repurposed drug library specifically induce MISR2 signaling, trigger regression of duct, inhibit follicle activation. test these vivo show they can repress folliculogenesis mice...
Summary Anti-Müllerian hormone (AMH) protects the ovarian reserve from chemotherapy, and this effect is most pronounced with Doxorubicin (DOX). However, mechanisms of DOX toxicity AMH rescue in ovary remain unclear. Herein, we characterize these various cell types using scRNAseq. In mesenchyme, activates intrinsic apoptotic signaling pathway through p53 class mediators, particularly affecting theca progenitors, while co-treament halts differentiation reduces gene expression. preantral...