A5080103950- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Sesquiterpenes and Asteraceae Studies
- Enzyme Catalysis and Immobilization
- Biochemical and biochemical processes
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Food Quality and Safety Studies
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Seaweed-derived Bioactive Compounds
- Light effects on plants
- Plant nutrient uptake and metabolism
- Alkaloids: synthesis and pharmacology
- Genetically Modified Organisms Research
- Plant Reproductive Biology
- Ruminant Nutrition and Digestive Physiology
- Piperaceae Chemical and Biological Studies
- Plant Toxicity and Pharmacological Properties
- Lung Cancer Research Studies
- Moringa oleifera research and applications
- Plant-Derived Bioactive Compounds
Gyeongsang National University
2018-2025
University of Calgary
2014-2024
The Netherlands Cancer Institute
2018
Calgary Laboratory Services
2018
Seoul National University
2013-2017
Kangwon National University
2007
The rumen plays an indispensable role in ruminants to utilize ligno-cellulosic material and convert non-protein nitrogen into nutrients otherwise unavailable for human consumption. Recent advancements the sequencing technology omics approach have provided profound insights world, wherein a consortium of archaea, bacteria, protozoa, fungi, viruses exist interact. These ruminal microbes alter environment execute several interlinked metabolic cascades that produce substrates host’s energy body...
Plants are the richest source of specialized metabolites. The metabolites offer a variety physiological benefits and many adaptive evolutionary advantages frequently linked to plant defense mechanisms. Medicinal plants vital nutrition active pharmaceutical agents. production valuable bioactive compounds has increased with improvement transgenic techniques like gene silencing overexpression. These beneficial for decreasing costs increasing nutritional value. Utilizing biotechnological...
Methane emission from enteric rumen fermentation is a main source of greenhouse gas (GHG) and major concern for global warming. In this study, we isolated methanotroph-methylotroph consortium NC52PC the after series sub-culture repetitive streaking on an agar plate polycarbonate membrane filter. The comprises methanotroph species (Methylocystis sp.) methylotroph (Methylobacterium sp.), forming capable growing solely methane as carbon source. Their morphology, growth, genome sequence were...
Natural rubber (cis-1,4-polyisoprene) is an indispensable biopolymer used to manufacture diverse consumer products. Although a major source of natural the tree (Hevea brasiliensis), lettuce (Lactuca sativa) also known synthesize rubber. Here, we report that unusual cis-prenyltransferase-like 2 (CPTL2) lacks conserved motifs conventional cis-prenyltransferase required for biosynthesis in lettuce. CPTL2, identified from particle proteome, displays homology human NogoB receptor and...
Salvia divinorum commonly known as diviner's sage, is an ethnomedicinal plant of the mint family (Lamiaceae). rich in clerodane-type diterpenoids, which accumulate predominantly leaf glandular trichomes. The main bioactive metabolite, salvinorin A, first non-nitrogenous natural compound to function opioid-receptor agonist, and undergoing clinical trials for potential use treating neuropsychiatric diseases drug addictions. We report here discovery functional characterization two S. diterpene...
Summary Lettuce produces natural rubber (NR) with an average M w of > 1 million Da in laticifers, similar to NR from trees. As lettuce is annual, self‐pollinating, and easily transformable plant, it excellent model for molecular genetic studies biosynthesis. CRISPR/Cas9 mutagenesis was optimized using hairy roots, NR‐deficient generated via bi‐allelic mutations cis ‐prenyltransferase ( CPT ). This the first null mutant deficiency plants. In mutant, orthologous counterparts guayule...
Guayule (Parthenium argentatum) is a perennial shrub in the Asteraceae family and synthesizes high quality, hypoallergenic cis-1,4-polyisoprene (or natural rubber; NR). Despite its potential to be an alternative NR supplier, enzymes for cis-polyisoprene biosynthesis have not been comprehensively studied guayule. Recently, implications of protein complex involving cis-prenyltransferases (CPTs) CPT-Binding Proteins (CBPs) were shown lettuce dandelion, but such complexes yet examined Here we...
(−)-α-Bisabolol, a sesquiterpene alcohol, is major ingredient in the essential oil of chamomile (Matricaria recutita) and used many health products. The current supply (−)-α-bisabolol mainly dependent on Brazilian candeia tree (Eremanthus erythropappus) by distillation or chemical synthesis. However, method using not sustainable, synthesis suffers from impurities arising undesirable α-bisabolol isomers. Therefore enzymatic viable alternative. In present study, cDNA encoding synthase (MrBBS)...
Abstract In plants, balancing growth and environmental responses is crucial for maximizing fitness. Close proximity among plants canopy shade, which negatively impacts reproduction, elicits morphological adjustments such as hypocotyl leaf hyponasty, mainly through changes in light quality auxin levels. However, how auxin, synthesized from a shaded blade, distally induces elongation of petiole cells remains to be elucidated. We demonstrated that ASYMMETRIC LEAVES1 (AS1) promotes hyponasty the...
Drimenol, a sesquiterpene alcohol, and its derivatives display diverse bio‐activities in nature. However, drimenol synthase gene has yet to be identified. We identified new cDNA ( VoTPS3 ) valerian plant Valeriana officinalis ). Purification NMR analyses of the VoTPS3‐produced terpene, characterization enzyme confirmed that synthesizes (−)‐drimenol. In feeding assays, possible reaction intermediates, farnesol drimenyl diphosphate, could not converted drimenol, suggesting intermediate remains...
Sesquiterpenoids are one of the most diverse families natural compounds with various bioactivities and functions. The introduction an exogenous mevalonate pathway was recognized to be proficient approach in Escherichia coli for sesquiterpene biosynthesis. It is challenging from coordination constituents forge active pathway, especially balance generation consumption by top bottom portions pathway. In this study, were categorized hierarchically assemble which optimized a Kronecker product...
Adaptive evolution of enzymes benefits from catalytic promiscuity. Sesquiterpene lactones (STLs) have diverged extensively in the Asteraceae, and studies for two representative STLs, costunolide artemisinin, could provide an insight into adaptive enzymes. Costunolide appeared early Asteraceae is widespread, whereas artemisinin a unique STL appearing single species, Artemisia annua. Therefore, ubiquitous STL, while specialized one. In biosynthesis, germacrene A oxidase (GAO) synthesizes acid...
Abstract Piperonal is a simple aromatic aldehyde compound with characteristic cherry-like aroma and has been widely used in the flavor fragrance industries. Despite piperonal being an important black pepper ( Piper nigrum ), its biosynthesis remains unknown. In this study, bioinformatic analysis of P. transcriptome identified novel hydratase-lyase, displaying 72% amino acid identity vanillin synthase, member cysteine proteinase family. vivo substrate-feeding vitro enzyme assays,...
Background: Activated Cdc42-associated kinase (ACK1) is essential for numerous cellular functions, such as growth, proliferation, and migration. ACK1 signaling occurs through multiple receptor tyrosine kinases; therefore, its inhibition can provide effective antiproliferative effects against human cancers. A number of ACK1-specific inhibitors were designed discovered in the previous decade, but none have reached clinic. Potent selective are urgently needed. Methods: In present investigation,...
Floral transition from the vegetative to reproductive stages is precisely regulated by both environmental and endogenous signals. Among these signals, photoperiod one of most important factors for onset flowering. A florigen, FLOWERING LOCUS T ( FT ) in Arabidopsis , has thought be a major hub photoperiod-dependent flowering time regulation. Expression levels likely correlates with potence Under long days (LD), mainly synthesized leaves, protein moves shoot apical meristem (SAM) where it...