A5075287357- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Diagnosis and treatment of tuberculosis
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- Synthesis and Biological Evaluation
- Probiotics and Fermented Foods
- Bioactive Compounds and Antitumor Agents
- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Infectious Diseases and Tuberculosis
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Respiratory viral infections research
- Viral-associated cancers and disorders
- Bacterial Infections and Vaccines
- CRISPR and Genetic Engineering
- Reproductive tract infections research
Federal Medical-Biological Agency
2020-2025
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2019-2024
Background The combined use of bacteriophages and antibiotics represents a promising strategy for combating multidrug-resistant bacterial pathogens. However, the lack uniformity in methods assessing combination effects experimental protocols has resulted inconsistent findings across studies. This study aimed to evaluate interactions between phages on Klebsiella pneumoniae strains using various statistical approaches formalize effects. Methods Effects were assessed four from distinct classes...
Abstract Bacteriophage therapy is considered one of the most promising therapeutic approaches against multi-drug resistant bacterial infections. Infections caused by Staphylococcus aureus are very efficiently controlled with bacteriophage cocktails, containing a number individual phages infecting majority known pathogenic S. strains. We assessed contribution bacteriophages comprising cocktail in order to optimize its composition. Two lytic vB_SauM-515A1 ( Myoviridae ) and vB_SauP-436A...
Reductive genome evolution is one of the most important and intriguing adaptation strategies different living organisms to their environment. Mycobacterium offers several notorious examples either naturally reduced ( leprae ) or laboratory-reduced bovis BCG) genomes. tuberculosis complex has its phylogeny unambiguously framed by large sequence polymorphisms that present unidirectional unique event changes. In study, we curated all known regions difference analyzed both animal-adapted MTBC species.
Antibiotic resistance is a major public health concern in many countries worldwide. The rapid spread of multidrug-resistant (MDR) bacteria the main driving force for development novel non-antibiotic antimicrobials as therapeutic alternative. Here, we isolated and characterized three virulent bacteriophages that specifically infect lyse MDR
To date, transcriptomics have been widely and successfully employed to study gene expression in different cell growth phases of bacteria. Since bifidobacteria represent a major component the gut microbiota healthy human that is associated with numerous health benefits for host, it important them using transcriptomics. In this study, we applied RNA-Seq technique global B. longum at order better understand response bifidobacterial cells specific conditions gut. We shown lag phase, ABC...
The development of whole-genome sequencing technologies is gradually leading to a more detailed description the population structure Mycobacterium tuberculosis complex (MTBC). In this study, we correlated previously published classifications on collection than 10,000 genomes and proposed new, comprehensive nomenclature that unifies existing ones. total, identified 169 lineages sublineages M. tuberculosis/M. africanum 9 animal-adapted species. For purpose organizing these genotypes in...
Mycobacteriophages are viruses that specifically infect bacteria of the Mycobacterium genus. A substantial collection mycobacteriophages has been isolated and characterized, offering valuable insights into their diversity evolution. This also holds significant potential for therapeutic applications, particularly as an alternative to antibiotics in combating drug-resistant bacterial strains. In this study, we report isolation characterization a new mycobacteriophage, Vic9, using smegmatis mc...
In 2022, the World Health Organization reported that tuberculosis (TB) was second leading cause of death globally from a single infectious agent, following COVID-19. The development new antitubercular agents with novel mechanisms action for use in complex TB therapy is considered key approach to combating TB. this study, we examined gene expression profile M. smegmatis when exposed promising antituberculosis quinoxaline 1,4-dioxide (QdNO)...
In 2022, the World Health Organization reported that tuberculosis (TB) was second leading cause of death globally from a single infectious agent following COVID-19. The development new antitubercular agents with novel mechanisms action for use in complex TB therapy is considered key approach to combating TB. this study, we examined gene expression profile M. smegmatis when exposed promising antituberculosis agent, quinoxaline 1,4-dioxide (QdNO)...
G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known G4-stabilizing ligands. While some of these ligands shown in vitro to have a stabilizing effect, the precise mechanism antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing analyze response Mycobacterium smegmatis inhibitory concentrations BRACO-19 and TMPyP4 G4 The expression profile changed (FDR...
. The Mycobacterium tuberculosis Beijing genotype is globally spread lineage with important medical properties that however vary among its subtypes. M. 14717-15-cluster was recently discovered as both multidrug-resistant, hypervirulent, and highly-lethal strain circulating in the Far Eastern region of Russia. Here, we aimed to analyze pathogenomic features phylogeographic pattern.
In light of the ever-increasing number multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host must be thoroughly investigated. Here, we report genome-wide transcriptional changes in clinical Staphylococcus aureus SA515 strain for three time points after infection vB_SauM-515A1 kayvirus. Using an RNA sequencing approach, identify 263 genes that were differentially expressed (DEGs) between phage-infected...
The Twort-like myoviruses (
Tuberculosis, caused by Mycobacterium tuberculosis complex bacteria, remains one of the most pressing health problems. Despite general trend towards reduction disease incidence rate, situation extremely tense due to distribution resistant forms. Most often, these strains emerge through intra-host microevolution pathogen during treatment failure. In present study, focus was on three serial clinical isolates Beijing B0/W148 cluster from patient with pulmonary tuberculosis, evaluate their...
In order to address the upcoming crisis in treatment of
Mycobacterium tuberculosis Beijing B0/W148 is one of the most widely distributed clusters in Russian Federation and some countries former Soviet Union. Recent studies have improved our understanding reasons for "success" cluster but this area remains incompletely studied. Here, we focused on system omics analysis RUS_B0 strain belonging to cluster. Completed genome sequence (CP020093.1) a collection WGS 394 strains were used describe main genetic features population. In turn, proteome...
The Klebsiella pneumoniae bacterium is capable of causing the broad range human nosocomial infections associated with antibiotic resistance and high mortality. Virulent bacteriophage therapy one promising alternatives to treatment such infections. study was aimed isolate virulent bacteriophages effective against relevant clinical K. strains, perform molecular genetic characterization these phages. Bacteriophages were isolated from river water samples using enrichment method. whole-genome...
In the context of antimicrobial therapy crisis, significance studying and implementing alternative treatment methods, particularly phage therapy, is increasingly evident. This study aimed to investigate resistance clinical Staphylococcus aureus ST239 strains Herelleviridae phages through comparative genomics, transcriptomics, proteomics. Analysis resistant sensitive S. showed that form a separate cluster on phylogenetic tree, suggesting unique genetic traits underlying their resistance....
As permanent residents of the normal gut microbiota, bifidobacteria have evolved to adapt host's immune response whose priority is eliminate pathogenic agents. The mechanisms that ensure survival commensals during inflammation and maintain stability core component microbiota in such conditions remain poorly understood. We propose a new vitro approach study resistance factors based on high-performance sequencing followed by transcriptome analysis. This allowed us detect differentially...
Phage therapy is now seen as a promising way to overcome the current global crisis in spread of multidrug-resistant bacteria. However, phages are highly strain-specific, and most cases one will have isolate new phage or search for suitable therapeutic application existing libraries. At an early stage isolation process, rapid screening techniques needed identify type potential virulent phages. Here, we propose simple PCR approach differentiate between two families Staphylococcus...
Mycobacterium tuberculosis Beijing genotype associated with drug resistance is a growing public health problem worldwide. The aim of this study was the assessment virulence for C57BL/6 mice after infection by clinical M. strains 267/47 and 120/26, which belong to modern sublineages B0/W148 Central Asia outbreak genotype, respectively. were identified analysis strains' whole-genomes. 120/26 characterized as agents pre-extensively drug-resistant (pre-XDR) multidrug-resistant (MDR)...
<h3></h3> Вирусом Эпштейна—Барр (ВЭБ) инфицировано около 90% населения планеты. Ущерб здоровью и высокие экономические потери требуют разработки вакцин против ВЭБ-инфекции. Вариабельность отдельных генов может повлиять на успех прививочной кампании будет требовать динамического обновления антигенного состава вакцин. <h3>ЦЕЛЬ ИССЛЕДОВАНИЯ</h3> Оценка вариабельности <i>gp350</i> <i>EBNA2</i> ВЭБ, выделенного из слюны персонала стоматологических клиник московского региона. <h3>МАТЕРИАЛ И...
The growing proportion of antibiotic-resistant Klebsiella pneumoniae strains raises challenges to the healthcare system and requires development alternative treatment options. Bacteriophage therapy is one such study was aimed isolate describe bacteriophages effective against K. clinically significant capsular types. were isolated from sewage river water samples using enrichment culture technique. spectrum lytic activity phages tested on collection clinical isolates (n = 279). studied lysed...