A5023487020- Gut microbiota and health
- Infant Nutrition and Health
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Breastfeeding Practices and Influences
- Enterobacteriaceae and Cronobacter Research
- Child Nutrition and Water Access
- Pediatric health and respiratory diseases
- Gestational Diabetes Research and Management
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Bariatric Surgery and Outcomes
- Salmonella and Campylobacter epidemiology
- Obesity and Health Practices
- Diet, Metabolism, and Disease
- Infant Health and Development
- Pelvic floor disorders treatments
- Intestinal Malrotation and Obstruction Disorders
- Advanced Glycation End Products research
- Clinical Nutrition and Gastroenterology
- Infant Development and Preterm Care
- Tryptophan and brain disorders
- Streptococcal Infections and Treatments
Universidade de São Paulo
2016-2025
Hospital Universitário da Universidade de São Paulo
2018-2025
Institute of Biomedical Science
2024
Instituto de Ciências Farmacêuticas
2024
Instituto de Medicina Integral Professor Fernando Figueira
2017
Instituto Butantan
2007-2011
We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification DEC cannot be based on only biochemical culture criteria, we used multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic (STEC/EHEC), Enterotoxigenic...
Human milk microorganisms contribute not only to the healthy development of immune system in infants, but also shaping gut microbiota. We evaluated effect maternal diet during pregnancy and first month lactation on human microbiota a cross-sectional study including 94 lactating women. Microbiota composition was determined by 16S rDNA profiling nutrient intake assessed through food questionnaires. Thirteen genera were present at least 90% all samples, with three samples: Streptococcus,...
This study characterized 76 atypical enteropathogenic Escherichia coli (aEPEC) strains, previously classified by the eae(+) EAF-negative stx(-) genotype, isolated from children with diarrhea in Brazil. Presence of bfpA and bfpA/perA was detected 2 6 respectively. The expression bundle-forming pilus (BFP), however, observed immunofluorescence 1 3 classifying them as typical EPEC (tEPEC). remaining 72 aEPEC strains were serotyping, intimin typing, adherence patterns to HEp-2 cells, capacity...
Gut microbiota composition is influenced by environmental factors and has been shown to impact body metabolism. Objective: To assess the gut profile before after Roux-en-Y gastric bypass (RYGB) correlation with food intake postoperative type 2 diabetes remission (T2Dr). Design: from obese diabetic women was evaluated (n = 25) 3 20) 12 months 14) RYGB, using MiSeq Illumina-based V4 bacterial 16S rRNA gene profiling. Data on (7-day record) T2Dr (American Diabetes Association (ADA) criteria)...
Few studies reported the relation of intestinal microbiome composition and diversity in pediatric patients with primary sclerosing cholangitis (PSC) ulcerative colitis (UC). In this cross-sectional study, we selected younger than 19 years old from gastroenterology hepatology outpatient clinic a tertiary hospital to describe PSC associated or not UC. Patients were divided PSC, PSC+UC, UC diagnosis. A stool sample was collected each patient (n=30) healthy relative/neighbor (n=23). The assessed...
Enteropathogenic Escherichia coli (EPEC) express a plasmid-encoded type IV pilus termed bundle-forming pilus, which is associated with the formation of bacterial microcolonies on cultured epithelial cells. Bacterial attachment and effacement enterocyte brush border membrane attributed to surface outer protein adhesin intimin EPEC-secreted proteins EspA, EspB, EspD. Except for intimin, production in vivo or antibody response against these virulence determinants during natural EPEC infections...
Type 1 diabetes mellitus (T1DM) is a disorder resulting from chronic autoimmune destruction of insulin-producing pancreatic β-cells, lack insulin production and hyperglycaemia. The aim this study was to evaluate the hypothesis that streptozotocin-diabetic mice treated with Saccharomyces boulardii THT 500101 strain present improvement glucose triglycerides metabolism, reduction liver inflammation concomitant beneficial impact in gut microbiota profile. C57BL/6 male were randomly assigned into...
The initial colonization of the human microbiota is paramount importance. In this context, oropharyngeal administration colostrum a safe, viable, and well-tolerated practice even by smallest preterm infants. Therefore, study evaluated effects on establishment infants’ oral microbiota. A longitudinal observational was carried out with 20 premature neonates, divided into two groups: one receiving protocol (Oropharyngeal Administration Colostrum; OAC) other Standard Caare (SC). Saliva samples...
The prevalence of gestational diabetes mellitus (GDM) is a global public health concern. mechanism that leads to glucose tolerance beyond normal physiological levels pathogenic conditions remains incompletely understood, and it speculated the maternal microbiome may play an important role. This study analyzes gut microbiota composition in each trimester weight-matched women with without GDM examines possible bacterial genera associations GDM. followed 56 pregnant 59 admitted outpatient...
Metabolic syndrome is a multifactorial disease, and the gut microbiota may play role in its pathogenesis. Obesity, especially abdominal obesity, associated with insulin resistance, often increasing risk of type two diabetes mellitus, vascular endothelial dysfunction, an abnormal lipid profile, hypertension, inflammation, all which promote development atherosclerotic cardiovascular disease.To evaluate outcomes fecal transplantation (FMT) patients metabolic syndrome.This was randomized,...
This paper reports the methods and preliminary findings of Germina, an ongoing cohort study to identify biomarkers trajectories executive functions language development in first 3 years life. 557 mother-infant dyads (mean age mothers 33.7 years, 65.2% white, 48.7% male infants) have undergone baseline are currently collecting data for other timepoints. A linear regression was used predict Bayley-III using scores derived from data-driven sparse partial least squares utilizing a multiple...
This paper reports the methods and preliminary findings of Germina, an ongoing cohort study to identify biomarkers trajectories executive functions language development in first 3 years life. 557 mother-infant dyads (mean age mothers 33.7 years, 65.2% white, 48.7% male infants) have undergone baseline are currently collecting data for other timepoints. A linear regression was used predict Bayley-III using scores derived from data-driven sparse partial least squares utilizing a multiple...
Exclusive breastfeeding (EBF) plays a crucial role in infant gut microbiome assembly and development. However, few studies have investigated the effects of EBF restoring perturbed microbiome. In this study, we applied whole metagenomic sequencing to assess 525 Brazilian infants from 3 9 months age Germina Cohort, demonstrating early determinants microbial taxonomy function modulation. Our analysis shows that alters relative abundance genes related function, with varying by delivery mode....
OBJECTIVE:The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health.In this research, development fecal group exclusively breastfed neonates living low socio-economic conditions city Sa ˜o Paulo, Brazil, during first month life, was studied.METHODS: Fecal samples were collected from ten on second, seventh, and 30 th days after birth.One underwent antibiotic therapy.Molecular techniques used analysis; DNA extracted...