A5057883761- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Nanoparticle-Based Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Treatment and Pharmacology
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related Molecular Pathways
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- HER2/EGFR in Cancer Research
- Microtubule and mitosis dynamics
- Mass Spectrometry Techniques and Applications
- Fibroblast Growth Factor Research
- PI3K/AKT/mTOR signaling in cancer
- Lung Cancer Treatments and Mutations
- Glioma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
University at Buffalo, State University of New York
2016-2025
Roswell Park Comprehensive Cancer Center
2015-2024
Center for Excellence in Education
2007-2023
University at Albany, State University of New York
2022
Mayo Clinic
2021
Enercon (Germany)
2019-2020
State University of New York
1997-2019
Amherst College
2019
University of Pennsylvania
2009
New York State College of Agriculture & Life Sciences
2009
Negatively charged liposomes are endocytosed by the coated vesicle system and accumulate in acidic intracellular vesicles. Liposomes that become unstable at pH improve cytoplasmic delivery of membrane-impermeant macromolecules such as calcein (CAL) FITC dextran (18 or 40 kDa). Oleic acid (OA): phosphatidylethanolamine (PE) (3:7 mole ratio) permeable to CAL less than 7.0. Control phosphatidylserine:PE OA:phosphatidylcholine stable 4-8. OA:PE promote encapsulated CV-1 cells, evidenced...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton-induced fusion of oleic acid-phosphatidylethanolamine liposomesNejat Duzgunes, Robert M. Straubinger, Patricia A. Baldwin, Daniel S. Friend, and Demetrios PapahadjopoulosCite this: Biochemistry 1985, 24, 13, 3091–3098Publication Date (Print):June 18, 1985Publication History Published online1 May 2002Published inissue 18 June 1985https://pubs.acs.org/doi/10.1021/bi00334a004https://doi.org/10.1021/bi00334a004research-articleACS...
Candida albicans is the predominant species of yeast isolated from patients with oral candidiasis, which frequently a symptom human immunodeficiency virus infection and criterion for staging progression AIDS. Salivary histatins (Hsts) are potent in vitro antifungal agents have great promise as therapeutic humans candidiasis. The molecular mechanisms by Hsts kill cells not known. We report here, that unlike other antimicrobial proteins, do display lytic activities to lipid membranes, measured...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLiposome-mediated delivery of deoxyribonucleic acid to cells: enhanced efficiency by changes in lipid composition and incubation conditionsRobert Fraley, Robert M. Straubinger, Gordon Rule, E. Louise Springer, Demetrios PapahadjopoulosCite this: Biochemistry 1981, 20, 24, 6978–6987Publication Date (Print):November 1, 1981Publication History Published online1 May 2002Published inissue 1 November...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEndocytosis and intracellular fate of liposomes using pyranine as a probeRobert M. Straubinger, Demetrios Papahadjopoulos, Keelung HongCite this: Biochemistry 1990, 29, 20, 4929–4939Publication Date (Print):May 1, 1990Publication History Published online1 May 2002Published inissue 1 1990https://pubs.acs.org/doi/10.1021/bi00472a025https://doi.org/10.1021/bi00472a025research-articleACS PublicationsRequest reuse permissionsArticle...
For label-free expression profiling of tissue proteomes, efficient protein extraction, thorough and quantitative sample cleanup digestion procedures, as well sufficient reproducible chromatographic separation, are highly desirable but remain challenging. However, optimal methodology has remained elusive, especially for proteomes that rich in membrane proteins, such the mitochondria. Here, we describe a straightforward preparation procedure, coupled with selective sensitive nano-LC/Orbitrap...
The role of the 100-kDa polypeptide components clathrin-coated vesicles in endocytosis was investigated by microinjection specific monoclonal antibodies. Receptor-mediated uptake transferrin and liposomes quantitatively inhibited. These results show that polypeptides are directly involved localized clathrin assembly at cell periphery markers for endocytic pathway. This demonstrates an situ function these protein complexes which they found.
Liposomal formulations of anticancer agents have been developed to prolong drug circulating lifetime, enhance anti-tumor efficacy by increasing tumor deposition, and reduce toxicity avoiding critical normal tissues. Despite the clinical approval numerous liposome-based chemotherapeutics, challenges remain in development deployment micro- nano-particulate formulations, as well combining these novel with conventional drugs standard-of-care therapies. Factors requiring optimization include...
The tumor-suppressor p53 is commonly inactivated in colorectal cancer and pancreatic ductal adenocarcinoma, but existing treatment options for p53-mutant (p53
Mycobacterium avium is an intracellular pathogen that can invade and multiply within macrophages of the reticuloendothelial system. Current therapy not highly effective. Particulate drug carriers are targeted to system may provide a means deliver antibiotics more efficiently M. avium-infected cells. We investigated formulation ciprofloxacin azithromycin in liposomes tested their antibacterial activities vitro against residing J774, murine macrophage-like cell line. A conventional...
Mycobacterium avium is a facultative intracellular pathogen that can survive and replicate within macrophages. We tested the hypotheses survival mechanisms may include alteration of phagosomal pH or inhibition phagosome-lysosome fusion. M. was surface labeled with N-hydroxysuccinimidyl esters carboxyfluorescein (CF) rhodamine (Rho) to enable measurement individual avium-containing phagosomes interactions bacterium-containing secondary lysosomes. CF fluorescence sensitive, whereas Rho...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTaxol-Lipid Interactions: Taxol-Dependent Effects on the Physical Properties of Model MembranesSathyamangalam V. Balasubramanian and Robert M. StraubingerCite this: Biochemistry 1994, 33, 30, 8941–8947Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/bi00196a011https://doi.org/10.1021/bi00196a011research-articleACS PublicationsRequest reuse...
In animal models, liposomal formulations of paclitaxel possess lower toxicity and equal antitumor efficacy compared with the clinical formulation, Taxol. The goal this study was to determine formulation dependence pharmacokinetics in rats, order test hypothesis that altered biodistribution modifies exposure critical normal tissues. Paclitaxel administered intravenously either multilamellar (MLV) liposomes composed phosphatidylglycerol/phosphatidylcholine (L-pac) or Cremophor EL/ethanol...
Purpose: Insulin-like growth factor receptor 1 (IGF-1R) is critically involved in pancreatic cancer pathophysiology, promoting cell survival and therapeutic resistance. Assessment of IGF-1R inhibitors combination with standard-of-care chemotherapy, however, failed to demonstrate significant clinical benefit. The aim this work unravel mechanisms resistance inhibition develop novel strategies improve the activity therapies.Experimental Design: Growth screening lines was performed identify...
The use of CTX as a targeting agent for camptothecin-loaded polymeric nanoparticles directed against KRAS mutant CTX-resistant cancer cells was investigated. increased CPT internalisation into resulting in elevated cell death.