A5032582355- Cervical Cancer and HPV Research
- Gut microbiota and health
- Reproductive tract infections research
- Monoclonal and Polyclonal Antibodies Research
- Diet and metabolism studies
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- T-cell and Retrovirus Studies
- Nutritional Studies and Diet
- Nutrition, Genetics, and Disease
- NF-κB Signaling Pathways
- Genital Health and Disease
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Respiratory viral infections research
- Cancer-related gene regulation
- Advanced Breast Cancer Therapies
- Animal Disease Management and Epidemiology
- Cancer-related Molecular Pathways
- Pluripotent Stem Cells Research
- Inflammasome and immune disorders
- Toxin Mechanisms and Immunotoxins
- Genomics and Chromatin Dynamics
- Herpesvirus Infections and Treatments
- interferon and immune responses
Instituto Nacional de Salud Pública
2014-2025
Secretaria de Salud
2018
Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated cytokines. Vaginal microbiota determines presence of certain cytokines locally. We assessed association between cervical diversity and histopathological diagnosis each stage CC, we evaluated mRNA expression levels IL-4, IL-6, IL-10, TGF-β1, TNF-α IFN-γ across specific bacterial clusters. determined high throughput sequencing 16S rDNA amplicons classified...
Abstract Background Women with Human Papilloma Virus (HPV) persistence are characterized by high levels of IL-10 at cervix. We have determined whether polymorphisms gene promoter might be associated increased risk squamous intraepithelial cervical lesions (SICL) and exist significative differences mRNA expression cervix systemic serum protein between SICL cases non-Cervical Lesions (NCL). Methods Peripheral blood samples from (n = 204) NCL 166) were used to detect loci -592A/C (rs1800872),...
Alterations in the host cellular immune response allow persistent infections with High-Risk Human Papillomavirus (HR-HPV) and development of premalignant cervical lesions cancer (CC). Variations immunosuppressive cytokine levels cervix are associated natural history CC. To assess potential role genetic immunity cytokines serum risk developing CC, we conducted a case-control study paired by age.Peripheral blood samples from patients CC (n = 200) hospital controls 200), were used to evaluate...
The aetiological relationship between human papillomavirus (HPV) infection and cervical cancer (CC) is widely accepted. Our goal was to determine the prevalence of HPV types in Mexican women attending at Institute for Social Security from different areas Mexico.DNAs 2,956 samples were subjected genotyping: 1,020 with normal cytology, 931 low-grade squamous intraepithelial lesions (LGSIL), 481 high grade HGSIL 524 CC.Overall 67.1%. A total 40 found; HPV16 detected 39.4% HPV-positive followed...
To investigate the correlation among pro- or anti-inflammatory cytokines and two main gut microbiota phyla in obese children.Anthropometric data were obtained from 890 children under 14 years old to determine degree of obesity. Serum cytokine concentration was measured by ELISA. Relative abundance feces evaluated quantitative RealTime PCR assays.Anthropometric biochemical parameters statistically higher overweigth/ (OW/O) than lean (NW), Increased TNF-α levels found that also have a high...
COVID-19 was the leading cause of death in Mexico between 2020 and 2021. SARS-CoV-2 infection varies widely among individuals populations. Since variations genes related to immune response may play a role susceptibility outcome COVID-19, associations gene polymorphisms (SNPs) IL-6 (- 573G > C, rs1800796), TNF-α 308G A, rs1800629), IFN-γ 1615 C T, rs2069705) with expression levels these proteins nasopharynx serum were evaluated Mexican population mild, severe, or critical COVID-19. A total...
A persistent infection by high-risk human papillomavirus (HR-HPV) is a prerequisite for the development of cervical neoplasms; however, most studies have focused on risk factors associated with HPV-16 and HPV-18 only.We assessed association prevalence HPV-16, HPV-18, non-16/18 HR-HPV occurrence lesions in baseline cohort study HPV persistence Mexican population.Cross-sectional within 5-year dynamic women an abnormal cytology result from 2015 to 2021. DNA was detected using Anyplex II 28 kit....
The aim is to determine the prevalence of active infection by herpes simplex virus type 2 (HSV-2) among Mexican women with high-risk human papillomavirus (HR-HPV) cervical infection, recruited from public gynecology and colposcopy services. In a cross-sectional study, HSV-2 antibodies, DNA, HR-HPV DNA were quantified. Significant differences in seroprevalence rates found between negative positive cases. was 28.15% 16.1% (P = .0001), while 6.83% 0.62% .001) for groups, respectively. risk...
The AKNA gene is part of the 9q32 susceptibility locus for cervical cancer. A single-nucleotide polymorphism at codon 1119 AKNA, yields a biologically relevant amino acid change (R1119Q) DNA binding AT-hook motif. Genotype frequencies in 97 allele pairs were: R/R = 0.597, R/Q 0.278, Q/Q 0.123. homozygosity was present 8.33% healthy controls, 16.67% patients with intraepithelial neoplasia and 75% cancer patients. These differences are highly significant presence (p 0.01, odds ratio 3.66, 95%...
Cervical cancer (CC) is caused by a persistent infection of high-risk human papillomavirus (HR-HPV). While most HPV infections are transient, significant health problem in Mexico. With an estimated prevalence 10% among women reproductive age, approximately 25% these present at least positive result triage test, which according to previous studies expected be confirmed as CIN-2/3. The immune system has key role the natural history infection; alterations cellular response responsible for...
High-risk human papillomavirus (HPV) infection is the main risk factor for cervical cancer (CC) development, where continuous expression of E6 and E7 oncoproteins maintain malignant phenotype. In Mexico, around 70% CC cases are diagnosed in advanced stages, impacting survival patients. The aim this work was to identify biomarkers affected by HPV-16 that impact prognosis Expression profiles dependent on oncoproteins, as well their relationship with biological processes cellular signaling...
Persistent infection with high-risk Human Papillomavirus (HR-HPV) is the main requisite for cervical cancer development. Normally, HPV limited to site of and regulates a plethora cellular elements avoid immune surveillance by inducing an anti-inflammatory state, allowing progress through viral cycle carcinogenic process. Recent findings suggest that AT-hook transcriptional factor AKNA could play role in development cancer. strongly related expression co-stimulatory molecules such CD40/CD40L...
Cervical cancer (CC) is responsible for >260 000 deaths worldwide each year. Efforts are being focused on identifying genetic susceptibility factors, especially in genes related to the immune response. Akna has been proposed be one of them, but data regarding its functional role disease scarce. Supporting notion akna as a CC gene, we found two polymorphisms associated with squamous intraepithelial lesion (SIL) and CC; moreover, identified an association between high expression levels SIL,...
Durante el proceso de transformación las células normales a cancerosas, ocurren varias alteraciones genéticas. En este se presenta la pérdida del control los mecanismos replicación y reparación ADN, así como segregación material genético. Aunque tienen estrategias defensa contra desarrollo cáncer, tumorales activan diferentes vías escape que permiten progresión neoplasia. Avances recientes han permitido enfocar investigación cáncer hacia identificación algunos sus factores etiológicos. El...
The human akna gene encodes an AT-hook transcription factor, the expression of which is involved in various cellular processes. goal this study was to identify potential AKNA binding sites genes that participate T-cell activation and validate selected genes. Here we analyzed ChIP-seq microarray assays determine AKNA-binding motifs process altered by lymphocytes. In addition, performed a validation analysis RT-qPCR assess AKNA’s role promoting IL-2 CD80 expression. We found five AT-rich are...
Imbalance in the intestinal microbiota can lead to chronic low-grade inflammation. Diet may influence this association. In study, we aimed evaluate interaction between Akkermansia muciniphila (A. muciniphila) and dietary patterns using a proinflammatory index.We conducted cross-sectional study with school-aged children. We quantified relative abundance (RA) of A. feces polymerase chain reaction. collected information through employing food frequency questionnaire generated principal...
Chronic low-grade inflammation (CLI) is implicated in the development of multiple metabolic diseases. The gut microbiota (GM) activates different signaling pathways and induces phenotypic changes, offering an exciting opportunity to treat CLI. We evaluated mediation waist circumference on association GM with serum cytokines. In this cross-sectional study 331 children, we measured 5 bacterial species, namely, Lactobacillus (L.) casei, L. paracasei, reuteri, Staphylococcus (S.) aureus,...
Background: The alteration in the composition of gut microbiota has been associated with an increased risk developing cardiovascular and metabolic diseases. present study evaluated association between relative abundance (RA) intestinal Staphylococcus aureus inflammatory response cardiometabolic alterations children. Methods: This cross-sectional included 1142 children (age 6–12 years), which were classified by degree adiposity. Anthropometry, markers, RA S. measured. Cytokine concentrations...
Infection with high-risk human papillomavirus is required to develop cervical cancer. Some viruses modulate the Fas/FasL signaling evade immune response; role of these molecules in cancer not clear. In this study, we measured expression levels Fas and FasL mRNA, soluble proteins, cell surface proteins peripheral blood mononuclear cells from patients low- high-grade squamous intraepithelial lesions relation healthy women, gain new insights into development.Fas/FasL mRNA was tissues subjects;...
A polyphenol-rich diet modulates the abundance of Akkermansia muciniphila (A. muciniphila), protecting against metabolic disorders caused by several pathologies such as obesity. The aim this study was to determine if polyphenol intake and A. are related in Mexican children aged from 6 12 years old. To relative (RA) relate presence child population, we performed a Cross-sectional 1190 between age, all them with complete information anthropometry, biochemical parameters RA muciniphila....