Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage herbal products process comes into prominence. Lichens symbiotic organisms used for medicinal purposes years. There various anticancer treatments about components two lichen species present study. Objective Antitumor potential three secondary metabolites including olivetoric acid (OLA) physodic (PHA) isolated from Pseudevernia furfuracea (L.) Zopf...

Alpha-copaene (α-COP), a tricyclic sesquiterpene, is present in several essential oils of medicinal and aromatic plants has antioxidant antigenotoxic features. Its cytotoxic, cytogenetic oxidative effects have not been investigated neuron N2a neuroblastoma (NB) cell cultures. Therefore, we aimed to describe vitro: (i) cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenlytetrazolium bromide test; (ii) antioxidant/oxidant activity total capacity (TAC) status (TOS) analysis; (iii)...

ABSTRACT The present study was designed to investigate genotoxic and cytotoxic effects oxidative damage of increasing concentrations nano‐hydroxyapatite (5, 10, 20, 50, 75, 100, 150, 300, 500 1000 ppm) in primary human blood cell cultures. Cell viability detected by [3‐(4,5‐dimethyl‐thiazol‐2‐yl) 2,5‐diphenyltetrazolium bromide] assay lactate dehydrogenase release, while total antioxidant capacity stress levels were determined evaluate the injury. DNA also analyzed sister chromatid exchange,...

Farnesene (FNS) is an acyclic sesquiterpene. It has a wide range of important biological effects such as antioxidant, antimicrobial, and antifungal properties, although its cytotoxic, cytogenetic, oxidative have not been investigated in human blood tissue yet. To this aim, both MTT lactate dehydrogenase (LDH) assays were carried out to evaluate cell viability cytotoxicity. Total antioxidant capacity (TAC) total stress (TOS) parameters used assess alterations. In addition, micronucleus...

This paper describes the development of mitoxantrone-loaded PEGylated graphene oxide/magnetite nanoparticles (PEG-GO/Fe3O4-MTX), and investigation its preliminary drug delivery performance. For this, GO was synthesized through oxidizing graphite powder, subsequently carboxylated using a substitution nucleophilic reaction. The (GO-COOH) then conjugated with amine end-caped PEG chains by Steglich esterification. Afterward, GO-PEG/Fe3O4 nanocomposite anchoring Fe3O4 onto surface GO-PEG during...

Olanzapine (OLZ) is an atypical antipsychotic drug and commonly used for the treatment of schizophrenia bipolar disorder (BD). However, recent reports indicated that this could exhibit cytotoxic effects on nervous immune systems. To our knowledge, there scarce data considering genotoxic or oxidative damage potentials OLZ human lymphocyte culture system. Therefore, in study, potential (0 to 160 µM) have been evaluated whole blood cultures (WBCs) related status. Sister-chromatid exchange (SCE)...

Hydroxyapatite nanoparticles (HAP NPs) are widely used for preparations of biomedical and biotechnological fields such as drug delivery, gene therapy, molecular imaging. However, the current toxicological knowledge about HAP NPs is relatively limited. The present study was designed to investigate toxicity potentials various concentrations (0-1000 µg cm(-2)) in cultured primary rat hepatocytes. Cell viability detected by 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT)...

It is known that lichens are utilized for the treatment of many diseases including ulcer, diabetes, and cancer years. Secondary metabolites in structure provide various activity properties them. In present study, cytotoxic oxidative effects main constituents Pseudevernia furfuracea (L.) Zopf (Parmeliaceae), olivetoric acid (OA), physodic (PA) were investigated on cultured human amnion fibroblasts (HAFs). OA PA isolated from P. using column chromatography their structures determined by proton...

With the development of nanotechnology and wide use iron oxide nanoparticles, it has become necessary to assess potential adverse biological effects magnetite. This study investigated cytotoxicity, genotoxicity oxidative damage different concentrations magnetite (0 1000 mg/L) in human whole blood cultures. After supplementation magnetite, samples were incubated for 72 h. Cell viability was assessed by 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) lactate dehydrogenase...

The aim of this study was to investigate the protective effects olive leaf extract (OLE) on genotoxicity and oxidative damage in cultured human blood cells treated with permethrin (PM) presence a rat liver S9 mix containing cytochrome P 450 enzymes. Anti-genotoxic activities OLE were studied using sister chromatid exchange (SCE) chromosome aberration (CA) tests furthermore total antioxidant capacity (TAC) status (TOS) examined determine damage. Our results clearly revealed that treatment PM...

In this experimental design, we explored the neuroprotective potential of zingiberene (ZGB), a monocyclic sesquiterpene, in hydrogen peroxide (H2O2)-induced toxicity newborn rat cerebral cortex cell cultures for first time. The rats were exposed to H2O2 6 h determine oxidative stress levels. To evaluate viability, both 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays carried out. Total antioxidant capacity (TAC) total (TOS) parameters used...

The present study aims at assessing the efficacies of olivetoric acid (OA) and physodic (PA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) in human lymphocytes (HLs) vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide lactate dehydrogenase assays were performed to establish cytotoxicity HLs. Besides, oxidative stress genotoxicity monitored by estimating changes total (TOS) 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, respectively, At same time, OA-...

2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) induces hepatic damage. Propolis exhibits antioxidant properties and several studies suggest that supplementations with antioxidants can influence hepatotoxicity. Therefore, the aim of current study was to explore effectiveness propolis in alleviating toxicity TCDD liver rats. Animals were divided into six groups, namely, (0.75 8 µg/kg body weight (bw)), (50 mg/kg bw), µg) plus control, respectively. Rats intraperitoneally administered their...

Neuroblastoma (NB)cells are often used in cancer researches such as glioblastoma cells since they have the potential of high mitotic activity, nuclear pleomorphism, and tumor necrosis. Guaiazulene (GYZ 1,4-dimethyl-7-isopropylazulene)is present several essential oils medicinal aromatic plants. Many studies reported cytotoxic effect GYZ; however, there no that compare effects between cell lines normal human after treatment with GYZ.In this study, we aimed to describe vitro antiproliferative...

Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing of lichens that have shown numerous anticancer activities on glioblastoma so far. In present study, different concentrations water extract obtained from Usnea longissima Ach. were used order determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase...