A5069430373- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Plant Virus Research Studies
- Spectroscopy and Chemometric Analyses
- Spectroscopy Techniques in Biomedical and Chemical Research
- Gut microbiota and health
Shanghai East Hospital
2024-2025
Zhejiang Lab
2023-2025
Center for Life Sciences
2025
Tongji University
2024-2025
Tongji Hospital
2024-2025
Shanxi Medical University
2021
Sun Yat-sen University
2014
Abstract The powerful CRISPR genome editing system is hindered by its off-target effects, and existing computational tools achieved limited performance in genome-wide prediction due to the lack of deep understanding molecular mechanism. In this study, we propose incorporate dynamics (MD) simulations analysis system, present CRISOT, an integrated tool suite containing four related modules, i.e., CRISOT-FP, CRISOT-Score, CRISOT-Spec, CRISORT-Opti for RNA-DNA interaction fingerprint generation,...
Abstract Directed evolution of large enzymes is constrained by the immense combinatorial complexity amino acid sequences, complicating high-throughput screening efforts. Here, we develop a hybrid NMR-computational framework to accelerate enzyme engineering mapping functional hot spots and narrowing mutational landscape. The method involves NMR titrations with nanobodies substrates, detection chemical shift perturbations, acid-type determination coupled molecular docking, targeted...
The discovery of CRISPR-Cas systems has paved the way for advanced gene editing tools. However, traditional Cas methods relying on sequence similarity may miss distant homologs and aren't suitable functional recognition. With protein large language models (LLMs) evolving, there is potential system modeling without extensive training data. Here, we introduce CHOOSER (Cas HOmlog Observing SElf-processing scReening), an AI framework alignment-free with self-processing pre-crRNA capability using...
Antimicrobial resistance poses a growing threat to public health, emphasizing the urgent need for novel therapeutic strategies. peptides (AMPs), short peptide sequences with diverse mechanisms of action, offer promising alternative due their broad-spectrum activity against pathogens. Recent advances in protein language models (PLMs) have revolutionized structure prediction and functional annotation, highlighting potential AMP discovery development. In this context, we present AMP-SEMiner...
Abstract The discovery and functional annotation of CRISPR-Cas systems laid the groundwork for development novel CRISPR-based gene editing tools. Traditional similarity- search-based Cas strategies, which rely heavily on local sequence alignment reference homologs, may overlook a significant number remote homologs with limited similarity; it can not be applied directly recognition. With rapid protein large language models (LLMs), foundation are expected to help model without extensive...
We compare depth information from below the mucosa of ex vivo tissue to diagnose nasopharyngeal carcinoma (NPC) by NIR Raman spectroscopy. Spectral diagnosis performed on a PCA-LDA model achieves diagnostic sensitivity 99.3% (149/150), 100% (150/150), 98% (147/150) and 96.7% (145/150), specificity 96% (144/150) 87.3% (131/150) surface deeper levels using zero separate lines, respectively. The spectra discrimination results at different could reveal pathological development. Thus, deep level...
Objective To study the heteroplasmy of whole mitochondrial genome genotyping result hair shaft samples using HID Ion GeneStudioTM S5 Sequencing System. Methods The buccal swabs and blood 8 unrelated individuals, from different parts same individual were collected. Amplification was performed Precision ID mtDNA Whole Genome Panel. Analysis detection carried out Results DNA sequences in temporal 2 individuals showed heteroplasmy, while results swabs, blood, other 6 consistent. A total 119 base...