A5059411853- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Wnt/β-catenin signaling in development and cancer
- Lipid metabolism and biosynthesis
- Liver physiology and pathology
- Protein Tyrosine Phosphatases
- Cell death mechanisms and regulation
- Health and Medical Research Impacts
- Zebrafish Biomedical Research Applications
- Galectins and Cancer Biology
- RNA Research and Splicing
- Congenital heart defects research
- Academic Publishing and Open Access
- Cell Adhesion Molecules Research
- Muscle Physiology and Disorders
- Marine Invertebrate Physiology and Ecology
- scientometrics and bibliometrics research
- Angiogenesis and VEGF in Cancer
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
University of Copenhagen
2023-2024
National University of Singapore
2019-2024
Novo Nordisk (United States)
2024
Novo Nordisk Foundation
2023-2024
Broad Institute
2024
Foundation Center
2024
Abstract YAP, an effector of the Hippo signalling pathway, promotes organ growth and regeneration. Prolonged YAP activation results in uncontrolled proliferation cancer. Therefore, exogenous regulation activity has potential translational applications. We present a versatile optogenetic construct (optoYAP) for manipulating localisation, consequently its function. attach LOV2 domain that photocages nuclear localisation signal (NLS) to N‐terminus YAP. In 488 nm light, unfolds, exposing NLS,...
Skeletal myogenesis is dynamic, and it involves cell-shape changes together with cell fusion rearrangements. However, the final muscle arrangement highly organized striated fibers. By combining live imaging quantitative analyses, we dissected fast-twitch myocyte within zebrafish myotome in toto. We found a strong mediolateral bias timing; however, at cellular scale, there was heterogeneity shape relationship between initial position of fast myocytes resulting partners. show that expression...
Endothelial Cells (ECs) form cohesive cellular lining of the vasculature and play essential roles in both developmental processes pathological conditions. Collective migration proliferation endothelial cells are key underlying endothelialization vessels as well vascular graft, but complex interplay mechanical biochemical signals regulating these still not fully elucidated. While surface topography modifications have been used to enhance vitro, thus far such single-modality met with limited...
In a previous study, it was reported that Yap1 and Wwtr1 in zebrafish regulates the morphogenesis of posterior body epidermal fin fold (Kimelman et al., 2017). We report here DNA damage induces apoptosis basal cells (EBCs) yap1 -/- ;wwtr1 embryos. Specifically, these mutant EBCs exhibit active Caspase-3, Caspase-8, γH2AX, consistent with serving as stimulus extrinsic apoptotic pathway cells. Live imaging reveals steady growth cell size developing embryo, but this is inhibited followed by...
Abstract Symmetry breaking of protein distribution and cytoskeleton organization is an essential aspect for development apico-basal polarity. In embryonic cells this process largely cell autonomous, while differentiated epithelial collectively polarize during epithelium formation. We report here that the de novo polarization mature hepatocytes a autonomous process. Single developed bona fide secretory hemi-apical lumens upon adhesion to finely tuned substrates bio-functionalized with...
Rationale Failure of small synthetic vascular grafts is largely due to late endothelialization and has been an ongoing challenge in the treatment cardiovascular diseases. Objective Previous strategies developed promote graft include surface topographical modulation biochemical modifications. However, these have met with limited success. Importantly, although integrity Endothelial Cell (EC) monolayer crucial for endothelialization, crosstalk between topography cell-cell connectivity still not...
Abstract Background Tissues need to regenerate restore function after injury. Yet, this regenerative capacity varies significantly between organs and species. For example, in the heart, some species retain full throughout their lifespan but human cardiac cells display limited ability repair After a myocardial infarction, of cardiomyocytes is impaired reduces heart pump, causing failure. Therefore, there an injured post infarction. We investigate cell culture role YAP, transcriptional...
Abstract YAP, an effector of the Hippo signalling pathway, promotes organ growth and regeneration. Prolonged YAP activation results in uncontrolled proliferation cancer. Therefore, exogenous regulation activity has potential translational applications. We present a versatile optogenetic construct (optoYAP) for manipulating localisation, consequently its function. attached LOV2 domain that photocages nuclear localisation signal (NLS) to N-terminus YAP. In 488 nm light, unfolds, exposing NLS,...
ABSTRACT In a previous study, it was reported that Yap1 and Wwtr1 in zebrafish regulates the morphogenesis of posterior body epidermal fin fold (Kimelman, D., et al. 2017). We report here DNA damage induces apoptosis basal cells (EBCs) yap1 −/− ;wwtr1 embryos. Specifically, these mutant EBCs exhibit active Caspase-3, Caspase-8 γH2AX, consistent with serving as stimulus extrinsic apoptotic pathway cells. Live imaging reveals steady growth cell size developing embryo, but this is inhibited...