A5045544586- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Poxvirus research and outbreaks
- Toxoplasma gondii Research Studies
- Salmonella and Campylobacter epidemiology
- Enzyme Structure and Function
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- Metabolism and Genetic Disorders
- Plant Virus Research Studies
- Hemophilia Treatment and Research
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Amino Acid Enzymes and Metabolism
- Bacteriophages and microbial interactions
- RNA modifications and cancer
Imperial College London
2010-2024
Nova Southeastern University
2022
St Mary's Hospital
1997-2021
Heidelberg University
2021
University Hospital Heidelberg
2021
Royal Brompton & Harefield NHS Foundation Trust
2009
UCL Australia
2009
University of Oxford
1982-1996
John Radcliffe Hospital
1988
Institute for Molecular Medicine
1988
Vaccinia infection interferes with the presentation of influenza Haemagglutinin (HA) and Nucleoprotein (NP) to class I-restricted CTL. The inhibitory effect is selective for certain epitopes, more profound during late phase infection. For A/NT/60/68 NP, block present both early phases infection, COOH-terminal epitope defined by peptide 366-379, having no detectable on NH2-terminal 50-63. HA inhibited only vaccinia proteins, partially or completely restored expression rapidly degraded protein...
We describe the effect of proteasome specific inhibitor lactacystin on metabolic stability influenza nucleoprotein (NP) and generation antigens presented by human murine class I molecules major histocompatibility complex to cytotoxic T lymphocytes (CTL). show that cells treated with fail present influenza-specific CTL, but retain capacity defined epitopes expressed as peptides intracellularly recombinant vaccinia viruses. This block in antigen presentation can be overcome expressing viral...
To understand the time course of action any small molecule inside a single cell, one would deposit defined amount cell and initiate its activity at moment. An elegant way to achieve this is encapsulate in micrometer-sized reservoir, introduce it into remotely open wall by laser pulse, then follow biological response microscopy. The validity approach validated here using microcapsules with walls that are doped metallic nanoparticles so as enable them be opened an infrared laser. capsules...
Journal Article The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species Get access David H.L. Bishop, Bishop Department Microbiology. Medical Center, University Alabama in BirminghamBirmingham, AL 35294, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Keith G. Gould, Gould *Sir William Dunn School Pathology, OxfordSouth Parks Road, 0X1 3RE, UK Hiroomi Akashi, Akashi Corrie M. Clerx-van Haaster Nucleic Acids...
Objective Salmonella enterica infections can lead to Reactive Arthritis (ReA), which exhibit an association with human leucocyte antigen (HLA)-B*27:05, a molecule prone misfolding and initiation of the unfolded protein response (UPR). This study examined how HLA-B*27:05 expression UPR affect life-cycle within epithelial cells. Methods Isogenic cell lines expressing two copies either control HLA-B*35:01 heavy chain (HC) were generated determine effect on infection life-cycle. A line...
Abstract Following uptake by macrophages, live mycobacteria initially reside within an immature phagosome that resists acidification and retains access to recycling endosomes. Glycolipids are exported from the mycobacterial become available for immune recognition CD1-restricted T cells. The aim of this study was explore possibility lipoproteins might similarly escape act as targets in cells infected with mycobacteria. We have focused on a 19-kDa lipoprotein Mycobacterium tuberculosis...
Human T cell lymphotropic virus type1(HTLV-1)causes HTLV-l-associated myelopathy/tropical spastic paraparesis (HAM/TSP). We used interferon- enzyme-linked immunospot assays with overlapping peptides spanning the entire HTLV-1proteome to test whether HTLV-l-specific CD8+ cells differed significantly in frequency or immunodominance hierarchy between patients HAM/TSP and asymptomatic carriers correlated provirus load. Tax was immunodominant target antigen. There no significant qualitative...
It has been suggested that receptor-ligand complexes segregate or co-localise within immune synapses according to their size, and this is important for receptor signaling. Here, we set out test the importance of complex dimensions surveillance target cells by human Natural Killer (NK) cells. NK cell activation regulated integrating signals from activating receptors, such as NKG2D, inhibitory KIR2DL1. Elongating NKG2D ligand MICA reduced its ability trigger activation. Conversely, elongation...
HLA-B27 forms misfolded heavy chain dimers, which may predispose individuals to inflammatory arthritis by inducing endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). This study was undertaken define role of UPR-induced ER-associated degradation (ERAD) pathway in disposal dimeric conformers. HeLa cell lines expressing only 2 copies a carboxy-terminally Sv5-tagged were generated. The ER stress-induced degradation-enhancing α-mannosidase-like 1 (EDEM1) overexpressed...
Significantly higher frequencies of tumor necrosis factor alpha- and interleukin-2-secreting human T-lymphotropic virus type 1 (HTLV-1)-specific CD4(+) T cells were present in the peripheral blood mononuclear HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients than those asymptomatic carriers with similar provirus loads. The data suggest that HTLV-1-specific play a role pathogenesis HAM/TSP.
Cytotoxic T-lymphocyte (CTL) clones specific for the influenza A/PR/8/34 virus hemagglutinin (HA) were isolated by priming CBA mice with a recombinant vaccinia expressing HA molecule. The epitopes recognized two of these clones, which CD8+, Kk restricted, and subtype specific, defined using combination viruses fragments synthetic peptides. One epitope is in HA1 subunit at residues 259 to 266 (numbering from initiator methionine), amino acid sequence FEANGNLI, other HA2 10 18 terminus...
A recombinant vaccinia has been designed to express amino acids 366-379 of influenza nucleoprotein, previously shown be the minimal epitope recognized by a class I-restricted cytotoxic T cell clone. Target cells infected with virus expressing this peptide are CTL as efficiently target complete nucleoprotein. The results imply existence transport system that constitutively passes products degraded proteins from cytoplasm into membrane-bound compartment cell.
Recent work has demonstrated that nonstimulatory endogenous peptides can enhance T cell recognition of antigen, but MHCI- and MHCII-restricted systems have generated very different results. TCRs need to interact with the peptide–MHC (pMHC), showing peptide specificity for activation enhancers or coagonists. In contrast, MHCI-restricted cells studied date show no such coagonists, suggesting CD8 binding noncognate MHCI is more important. Here we how this dichotomy be resolved by varying TCR...
We have synthesized four different 5'-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotection and partial purification the oligoribonucleotides could be converted capped (m7Gppp) using guanylyl transferase. Radiolabelled acted as primers for influenza A virus RNA polymerase vitro. The method described here should also allow addition of 5'-diphosphates synthetic oligodeoxyribonucleotides capable automation.
We have constructed two chimeric influenza hemagglutinin (HA) genes in which the HA1 and HA2 subunits of HA molecule been interchanged between A/PR/8/34 (H1 subtype) A/NT/60/68 (H3 subtype). These were used to construct recombinant vaccinia viruses that expressed intact HA. test whether murine CTL recognize antigenic determinants either HA1, HA2, or both subunits. found contain for CTL. This implies have, at least part, separate from B lymphocytes, mainly epitopes within subunit.
Summary The potency of CD8 + cytotoxic T lymphocyte (CTL) responses toward core antigen has been shown to affect the outcomes hepatitis B virus (HBV) infection. Since single‐chain trimers (SCT) composed peptide epitope β 2 ‐microglobulin (β m) and major histocompatiblity complex (MHC) class I heavy chain covalently linked together in a single molecule have stimulate efficient CTL responses, we investigated properties human leucocyte (HLA)‐A2 SCTs encoding HBV (HBcAg) epitopes C 18−27 107−115...