A5078150129- Gene Regulatory Network Analysis
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- bioluminescence and chemiluminescence research
- Hippo pathway signaling and YAP/TAZ
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Diffusion and Search Dynamics
- Optical Imaging and Spectroscopy Techniques
- Estrogen and related hormone effects
- Invertebrate Immune Response Mechanisms
- Neural dynamics and brain function
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Evolutionary Algorithms and Applications
- Animal Behavior and Reproduction
University of Crete
2022
Karolinska Institutet
2022
Institute of Genetics and Cancer
2017-2020
University of Edinburgh
2017-2020
Max Planck Institute of Molecular Cell Biology and Genetics
2015-2020
Institute of Cell Biology
2020
Medical Research Council
2019
Max Planck Society
2015
University of Basel
2010-2012
RNA and membraneless organelles Membraneless compartments can form in cells through liquidliquid phase separation (see the Perspective by Polymenidou). But what prevents these cellular condensates from randomly fusing together? Using RNA-binding protein (RBP) Whi3, Langdon et al. demonstrated that secondary structure of different components determines distinct biophysical biological properties two types Whi3 forms. Several RBPs, such as FUS TDP43, contain prion-like domains are linked to...
Nonsense-mediated decay (NMD) is a translation-dependent RNA quality control mechanism that occurs in the cytoplasm. However, it unknown how NMD regulates stability of RNAs translated at endoplasmic reticulum (ER). Here, we identify localized pathway dedicated to ER-translated mRNAs. We previously identified NBAS, component Syntaxin 18 complex involved Golgi-to-ER trafficking, as novel factor. Furthermore, show NBAS fulfills an independent function NMD. This ER-NMD requires interaction with...
Transcription factor–DNA interactions are life sustaining and therefore the subject of intensive research. In spite vast effort, quantitative in vivo studies molecular mechanisms underlying these fundamental remain challenging. preceding paper, we designed synthetic Sex combs reduced (Scr) peptides validated genetically their function as transcriptional regulators. Here present a controllable system for protein–DNA live cells that enables us to “titrate” concentration Scr single cell. Using...
Functional fluorescence microscopy imaging (fFMI), a time-resolved (21 μs/frame) confocal technique without scanning, is developed for quantitative characterization of fast reaction-transport processes in solution and live cells. The method based on massively parallel correlation spectroscopy (FCS). Simultaneous excitation fluorescent molecules multiple spots the focal plane achieved using diffractive optical element (DOE). Fluorescence from DOE-generated 1024 illuminated detected...
ABSTRACT The variability in transcription factor concentration among cells is an important developmental determinant, yet how controlled remains poorly understood. Studies of have focused predominantly on monitoring mRNA production noise. Little information exists about protein variability, as this requires the use quantitative methods with single-molecule sensitivity. Using Fluorescence Correlation Spectroscopy (FCS), we characterized and 14 endogenously tagged TFs live Drosophila imaginal...
Homeotic ( Hox ) genes encode transcription factors that confer segmental identity along the anteroposterior axis of embryo. However molecular mechanisms underlying -mediated and differential requirements for specificity in regulation vast number -target remain ill-defined. Here we show synthetic Sex combs reduced Scr C terminus containing homedomain (HD) YPWM motif (Scr-HD) are functional vivo. Synthetic Scr-HD peptides can induce ectopic salivary glands embryo homeotic transformations...
Segmental identity along the anteroposterior axis of bilateral animals is specified by Hox genes. These genes encode transcription factors, harboring conserved homeodomain and, generally, a YPWM motif, which binds cofactors and increases transcriptional specificity in vivo. Here we derive synthetic Drosophila Antennapedia genes, consisting only motif homeodomain, investigate their functional role throughout development. Synthetic peptides full-length proteins cause head-to-thorax...
Epithelial organ size and shape depend on cell changes, cell–matrix communication, apical membrane growth. The Drosophila melanogaster embryonic tracheal network is an excellent model to study these processes. Here, we show that the transcriptional coactivator of Hippo pathway, Yorkie (YAP/TAZ in vertebrates), plays distinct roles developing airways. exerts a cytoplasmic function by binding Twinstar, orthologue vertebrate actin-severing protein Cofilin, regulate F-actin levels size, which...
Sensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. This precision emerges from biochemical processes within between cells that inherently stochastic. We investigated impact of stochastic gene expression on pattern formation, focusing senseless (sens), a key determinant sensory fate Drosophila. Perturbing microRNA regulation or genomic location sens produced distinct noise signatures. Noise was greatly enhanced when both alleles...
Most organs of multicellular organisms are built from epithelial tubes. To exert their functions, tubes rely on apico-basal polarity, junctions, which form a barrier to separate the inside outside, and proper lumen, required for gas or liquid transport. Here we identify apnoia (apn), novel Drosophila gene tracheal tube elongation lumen stability at larval stages. Larvae lacking Apn show abnormal inflation twisted airway tubes, but no obvious defects in early steps maturation. apn encodes...
Quantitative confocal fluorescence microscopy imaging without scanning is developed for the study of fast dynamical processes. The method relies on use massively parallel Fluorescence Correlation Spectroscopy (mpFCS). Simultaneous excitation fluorescent molecules across specimen achieved by passing a single laser beam through Diffractive Optical Element (DOE) to generate quadratic illumination matrix 32×32 light sources. from 1024 illuminated spots detected in arrangement matching detector...
Quantitative confocal fluorescence microscopy imaging without scanning is developed for the study of fast dynamical processes. The method relies on use massively parallel Fluorescence Correlation Spectroscopy (mpFCS). Simultaneous excitation fluorescent molecules across specimen achieved by passing a single laser beam through Diffractive Optical Element (DOE) to generate quadratic illumination matrix 32·32 light sources. from 1024 illuminated spots detected in arrangement matching detector...
Abstract Nonsense-mediated decay (NMD) is a translation-dependent RNA quality control mechanism that occurs in the cytoplasm. However, it unknown how NMD regulates stability of RNAs translated at Endoplasmic Reticulum (ER). Here, we identify localized pathway dedicated to ER-translated mRNAs. We previously identified NBAS, component Syntaxin 18 complex involved Golgi-to-ER trafficking, as novel factor. show NBAS fulfils an independent function NMD. This ER-NMD requires interaction with core...
Cell fate decisions can be envisioned as bifurcating dynamical systems, and the decision that Drosophila cells make during sensory organ differentiation has been described such. We extended these studies by focusing on Senseless protein which orchestrates cell transitions. Wing contain intermediate numbers before their transition, after they express much greater of molecules differentiate. However, dynamics are inconsistent with it being a simple bistable system. Cells best modeled residing...
Abstract Sensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. However, this precision must emerge from biochemical processes within between cells that stochastic. We measured intrinsic noise senseless protein output, a key determinant of sensory fate, during Drosophila development. Perturbing microRNA regulation or genomic locus transcription produced distinct signatures. Genomic location altered stochasticity an allelic-pairing...
The variability in transcription factor concentration among cells is an important developmental determinant, yet how controlled remains poorly understood. Studies of have focused predominantly on monitoring mRNA production noise. Little information exists about protein variability, since this requires the use quantitative methods with single-molecule sensitivity. Using Fluorescence Correlation Spectroscopy (FCS), we characterised and 14 endogenously tagged TFs live Drosophila imaginal discs....
Abstract Epithelial organ size and shape depend on cell changes, cell-matrix communication apical membrane growth. The Drosophila embryonic tracheal network is an excellent model to study these processes. Here, we show that the transcriptional co-activator of Hippo pathway, Yorkie (YAP in vertebrates), plays distinct roles developing airways. exerts a cytoplasmic function by binding Twinstar, orthologue vertebrate actin-severing protein Cofilin, regulate F-actin levels size, which are...