A5066332312- RNA Research and Splicing
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- 14-3-3 protein interactions
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- RNA regulation and disease
- HER2/EGFR in Cancer Research
- Endoplasmic Reticulum Stress and Disease
- Genomic variations and chromosomal abnormalities
- Monoclonal and Polyclonal Antibodies Research
- Radiomics and Machine Learning in Medical Imaging
- Neuroblastoma Research and Treatments
Karolinska Institutet
2008-2023
Stanford University
2019
Yale University
2013-2018
Howard Hughes Medical Institute
2016-2018
Transcription is a highly regulated process, and stress-induced changes in gene transcription have been shown to play major role stress responses adaptation. Genome-wide studies reveal prevalent beyond known protein-coding loci, generating variety of RNA classes, most unknown function. One such class, termed downstream gene-containing transcripts (DoGs), was reported result from transcriptional readthrough upon osmotic human cells. However, how widespread the phenomenon is, what its causes...
The p53 target gene Wig-1 encodes a double-stranded-RNA-binding zinc finger protein. We show here that binds to mRNA and stabilizes it through an AU-rich element (ARE) in the 3′ UTR of mRNA. This effect is mirrored by enhanced protein levels both unstressed cells exposed p53-activating stress agents. Thus, previously undescribed ARE-regulating acts as positive feedback regulator p53, with implications for steady-state response. Our data reveal link between tumor suppressor...
Wig-1, also known as ZMAT3, is a p53 target gene that encodes an RNA-binding zinc-finger protein involved in the regulation of mRNA stability through binding to AU-rich elements (AREs). We have used microarray analysis identify novel Wig-1 mRNAs. identified 2447 transcripts with >fourfold differential expression between and control small interfering (si)RNA-treated HCT116 cells. Several genes were among deregulated transcripts. found regulates FAS 14-3-3σ independently p53. show binds 3'-UTR...
Abstract Purpose: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide, and early detection significantly improves treatment outcomes, but existing blood-based tests often have limited sensitivity in early-stage disease. We developed a test combining oncRNAs, group small cell-free RNAs (cfRNAs), with generative AI to detect CRC. Experimental Design: leveraged cohort 613 CRC cases controls train model that demonstrated both high clinical performance as well...
Next-generation deep sequencing of gene panels is being adopted as a diagnostic test to identify actionable mutations in cancer patient samples. However, clinical samples, such formalin-fixed, paraffin-embedded specimens, frequently provide low quantities degraded, poor quality DNA. To overcome these issues, many assays rely on extensive PCR amplification leading an accumulation bias and artifacts. Thus, there need for targeted assay that performs well with DNA quantity without relying...
MINT‐6542926, MINT‐6542899: WIG1 (uniprotkb:Q9HA38) physically interacts (MI:0218) with hnRNP A2/B1 (uniprotkb:P22626) by anti bait coimmunoprecipitation (MI:0006) MINT‐6542945: RHA (uniprotkb:Q08211) MINT‐6542918, MINT‐6542891: MINT‐6542867: tag (MI:0007) MINT‐6542879:
// Cinzia Bersani 1 , Mikael Huss 2 Stefania Giacomello Li-Di Xu Julie Bianchi Sofi Eriksson Fredrik Jerhammar Andrey Alexeyenko 3 Anna Vilborg 4 Joakim Lundeberg Weng-Onn Lui Klas G. Wiman Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska, Stockholm, Sweden Science for Life Laboratory, School Biotechnology, Royal Institute Technology, Solna, Microbiology, Tumour and Cell biology, Bioinformatics Infrastructure Sciences, Molecular Biophysics Biochemistry, Yale...
The calcium ion (Ca2+) is a key intracellular signaling molecule with far-reaching effects on many cellular processes. One of the most important such Ca2+ regulated processes transcription. A body literature describes effect transcription initiation as occurring mainly through activation gene-specific factors by Ca2+-induced cascades. However, reach extends far beyond first step In fact, can regulate all phases transcription, additional transcription-associated events alternative splicing....
Wig-1 is a transcriptional target of the p53 tumor suppressor and encodes an mRNA stability-regulating protein. We show here that knockdown causes dramatic inhibition N-Myc expression triggers differentiation in neuroblastoma cells carrying amplified N-Myc. Transient significantly delays development N-Myc-driven tumors mice. also induced upon moderate p53-activating stress, suggesting role p53-Wig-1-N-Myc axis promoting cell cycle re-entry p53-induced arrest DNA repair. Moreover, our...
ABSTRACT Next-generation deep sequencing of gene panels is being adopted as a diagnostic test to identify actionable mutations in cancer patient samples. However, clinical samples, such formalin-fixed, paraffin-embedded specimens, frequently provide low quantities degraded, poor quality DNA. To overcome these issues, many assays rely on extensive PCR amplification leading an accumulation bias and artifacts. Thus, there need for targeted assay that performs well with DNA quantity without...
Abstract EBER2 is a highly abundant nuclear noncoding RNA expressed by Epstein-Barr virus (EBV). Probing its possible chromatin localization Capture Hybridization Analysis of Targets (CHART) revealed EBER2's presence at the terminal repeats (TRs) latent EBV genome, overlapping previously identified binding sites for important B-cell transcription factor PAX5. interacts with and required PAX5 to TRs. Knockdown or decreases lytic replication, underscoring essential role TRs in viral which...
Abstract Next Generation Sequencing is increasingly implemented as a diagnostic test to identify actionable mutations in cancer patient samples. However, for routine diagnostics, tumor DNA extracted from formalin-fixed, paraffin-embedded (FFPE) samples, which yields low quantity of damaged DNA. Inability accurately repair the ends these fragments impairs adapter ligation by standard double stranded methods. The resulting yield adapter-ligated introduces need whole-genome PCR amplification...
Abstract Next Generation Sequencing is increasingly used in translational cancer research and as a diagnostic test. Most tumor specimens available for testing are formalin-fixed, paraffin-embedded (FFPE) blocks. FFPE-derived DNA typically damaged, causing difficulties standard library preparation methods. Frayed ends prevent blunt-ended double-stranded adapter ligation, whole genome sequencing (WGS) targeted bait hybridization preparation. Fragmented modified bases interfere with PCR, which...
Abstract Background: Non-classical HER2 FISH results were recently reclassified in the 2018 guidelines update, and concurrent IHC testing was recommended as part of additional workup to determine final status these groups. In this study, we explored genomic landscape groups using digital droplet PCR (ddPCR) targeted next-generation sequencing (NGS) on invasive breast carcinomas. Methods: Fifty-one clinical samples with included our analysis classified into based updated ASCO/CAP guideline:...