A5100373871- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Alzheimer's disease research and treatments
- Protein Kinase Regulation and GTPase Signaling
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Corneal surgery and disorders
- Alcohol Consumption and Health Effects
- interferon and immune responses
- Planarian Biology and Electrostimulation
- Polyomavirus and related diseases
- Cell death mechanisms and regulation
- Computational Drug Discovery Methods
- Ocular Surface and Contact Lens
- Supramolecular Self-Assembly in Materials
- Protist diversity and phylogeny
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Inflammasome and immune disorders
- Skin and Cellular Biology Research
- RNA Interference and Gene Delivery
- Gout, Hyperuricemia, Uric Acid
- Cell Adhesion Molecules Research
Johns Hopkins Medicine
2018-2021
Johns Hopkins University
2018-2021
University of Baltimore
2021
Heilongjiang Academy of Sciences
2018
Harbin Medical University
2018
Kurume University
2014
CRISPR-Cas systems provide versatile tools for programmable genome editing. Here, we developed a caged RNA strategy that allows Cas9 to bind DNA but not cleave until light-induced activation. This approach, referred as very fast CRISPR (vfCRISPR), creates double-strand breaks (DSBs) at the submicrometer and second scales. Synchronized cleavage improved kinetic analysis of repair, revealing cells respond Cas9-induced DSBs within minutes can retain MRE11 after ligation. Phosphorylation H2AX...
Pyroptosis is a form of caspase-1-dependent programmed cell death with anti-tumor properties, but the underlying molecular mechanisms are not fully understood.The results our study showed that antihyperlipidemic drug simvastatin induced pyroptosis in non-small lung cancer (NSCLC) lines and xenograft mouse model.Inhibition attenuated effects on tumor viability migration.These data suggest may induce pyroptosis, thereby potentially serving as novel therapeutic agent for NSCLC.
Significance Cell migration is central in physiological and pathological conditions such as immune response cancer metastasis. The excitable network hypothesis can account for recent observations of propagating waves signal transduction cytoskeleton events well behaviors migrating cells. However, the molecular feedback loops involved these networks that bring about excitability are poorly understood. Here, we provide evidence a positive-feedback loop based on mutual inhibitory interaction...
Alcohol abuse exerts deleterious effects on the internal organs of body, and alcohol-related gastritis is a common disease for which prompt treatment essential to prevent condition from growing worse. However, therapeutic methods have some adverse effects. Determining pathogenic mechanisms alcoholic therefore essential.The MTT assay was developed in order determine optimal concentration alcohol needed treat gastric mucosal cells. The cells were determined by qRT-PCR western blot. release...
Abstract The ability of cells to polarize and move toward external stimuli plays a crucial role in development, as well normal pathological physiology. Migrating maintain dynamic complementary distributions Ras activity the phospholipid phosphatidylinositol‐3,4‐bisphosphate (PI(3,4)P2). Here, we show that lagging‐edge component PI(3,4)P2 also localizes retracting leading‐edge protrusions nascent macropinosomes, even absence phosphatidylinositol 3,4,5‐trisphosphate (PIP3). Once internalized,...