A5006943739- Anesthesia and Neurotoxicity Research
- Cardiac Arrest and Resuscitation
- Anesthesia and Sedative Agents
- Chemical Reactions and Isotopes
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- RNA modifications and cancer
- Traumatic Brain Injury and Neurovascular Disturbances
- Renal function and acid-base balance
- RNA Research and Splicing
- Viral-associated cancers and disorders
- Hormonal Regulation and Hypertension
- Pharmacological Effects of Natural Compounds
- Nuclear Structure and Function
- Lymphoma Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Parvovirus B19 Infection Studies
- Diet and metabolism studies
- HIV Research and Treatment
- Advanced Glycation End Products research
- PI3K/AKT/mTOR signaling in cancer
- Histiocytic Disorders and Treatments
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Genetic Neurodegenerative Diseases
- Cancer, Hypoxia, and Metabolism
Air Liquide (France)
2016-2024
Air Liquide (Canada)
2017-2024
Sanofi (France)
2011-2013
École Normale Supérieure de Lyon
1999-2003
Inserm
1999-2003
Institut de Biologie et de Chimie des Protéines
2003
Ludwig Cancer Research
2002
Class IA phosphoinositide 3-kinases (PI3Ks) are a family of p85/p110 heterodimeric lipid kinases that generate second messenger signals downstream tyrosine kinases, thereby controlling cell metabolism, growth, proliferation, differentiation, motility, and survival. Mammals express three class catalytic subunits: p110alpha, p110beta, p110delta. It is unclear to what extent these p110 isoforms have overlapping or distinct biological roles. Mice expressing catalytically inactive form p110delta...
A striking characteristic of mRNA export factors is that they shuttle continuously between the cytoplasm and nucleus. This shuttling mediated by specific interacting with peptide motifs called nuclear signals (NES) localization signals. We have identified a novel CRM-1-independent transferable NES two in Epstein-Barr virus factor EB2 (also BMLF1, Mta, or SM) localized at N terminus protein amino acids 61 146. also found previously described double (amino 213-236) does not mediate EB2, but an...
Despite its low chemical reactivity, the noble gas xenon possesses a remarkable spectrum of biological effects. In particular, is strong neuroprotectant in preclinical models hypoxic-ischemic brain injury. this study, we wished to determine whether retained neuroprotective potential experimental settings that model progressive loss midbrain dopamine (DA) neurons Parkinson's disease. Using rat cultures, established was partially protective for DA through either direct or indirect effects on...
ABSTRACT Human herpesviruses encode posttranscriptional activators that are believed to up-regulate viral replication by facilitating early and late gene expression. We have reported previously the Epstein-Barr virus protein EB2 (also called M or SM) promotes nuclear export of RNAs poor substrates for spliceosome assembly, an effect closely resembles human immunodeficiency type 1 Rev-dependent unspliced RNA. Here we present experimental data showing efficiently RNA expressed from a Rev...
The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced and partially spliced viral mRNA. In this study, we have identified new Rev-interacting protein, that specifically interacts with signal both in yeast mammalian cells. This has features found nucleoporins including many phenylalanine-glycine repeats, very high serine content, putative zinc finger, coiled-coil domain; thus called it NLP-1 (nucleoporin-likeprotein 1). addition, gene expression analysis wheat germ...
Abstract Parkinson's disease motor symptoms are treated with levodopa, but long‐term treatment leads to disabling dyskinesia. Altered synaptic transmission and maladaptive plasticity of corticostriatal glutamatergic projections play a critical role in the pathophysiology Because noble gas xenon inhibits excitatory signaling, primarily through allosteric antagonism N‐methyl‐ d ‐aspartate receptors, we aimed test its putative antidyskinetic capabilities. We first studied direct effect exposure...
Using midbrain cultures, we previously demonstrated that the noble gas xenon is robustly protective for dopamine (DA) neurons exposed to L-trans-pyrrolidine-2,4-dicarboxylate (PDC), an inhibitor of glutamate uptake used generate sustained, low-level excitotoxic insults. DA cell rescue was observed in conditions where control atmosphere culture substituted with a mix, comprising same amount oxygen (20%) and carbon dioxide (5%) but 75% instead nitrogen. In present study, first aimed determine...
Argon (Ar) is a noble gas with known organoprotective effects in rodents and vitro models. In previous study we failed to find postconditioning effect of Ar during ex vivo lung perfusion (EVLP) on warm-ischemic injury porcine model. this study, further investigated prolonged exposure decrease cold ischemia-reperfusion after transplantation model EVLP assessment. Domestic pigs (n = 6/group) were pre-conditioned for 6 hours 21% O 2 79% N (CONTR) or (ARG). Subsequently, lungs flushed stored...
Abstract The chemically inert noble gases display a surprisingly rich spectrum of useful biological properties. Relatively little is known about the molecular mechanisms behind these effects. It clearly not feasible to conduct large numbers pharmacological experiments on identify activity. Computational studies binding and proteins can address this paucity information provide insight into action. We used bespoke computational grid calculations predict positions energy minima in interactions...
The switch from latency to a productive cycle in Epstein–Barr virus (EBV)-infected B cells proliferating vitro is thought be due the transcriptional activation of two viral genes, BZLF1 and BRLF1, encoding transcription factors called EB1 R respectively. However, third gene, BRRF1 contained BZLF1/BRLF1 locus, overlapping with BRLF1 but inverse orientation. We have characterized 5′ end mRNA promoter, PNa, at which pre-mRNA initiated. show that although single species induced by 12- O...
Noble gases are chemically inert, and it was therefore thought they would have little effect on biology. Paradoxically, found that do exhibit a wide range of biological effects, many which target-specific potentially useful some been demonstrated in vivo. The underlying mechanisms by pharmacology, such as tissue neuroprotection, anti-addiction analgesia, is elicited relatively unexplored. Experiments to probe the interactions noble with specific proteins more difficult than those other...
New gas therapies using inert gases such as xenon and argon are being studied, which require in vitro vivo preclinical experiments. Examples of the kinetics transport during experiments analyzed this paper. Using analytical numerical models, we analyze an experiment for to a 96 cell well plate delivery small animal chamber, where key processes considered wash-in test into apparatus dead volume, diffusion through liquid media plate, pharmacokinetics rat. In case animals variable controlling...
Argon inhalation attenuates multiorgan failure (MOF) after experimental ischemic injury. We hypothesized that this protection could involve decreased High Mobility Group Box 1 (HMGB1) systemic release. investigated issue in an animal model of MOF induced by aortic cross-clamping. Anesthetized rabbits were submitted to supra-coeliac cross-clamping for 30 min, followed 300 min reperfusion. They randomly divided into three groups (n = 7/group). The Control group inhaled nitrogen (70%) and...
When administered in relatively high concentrations the mechanical properties of inhaled gas can become significantly different from air.This fact has implications ventilation where adequate respiration and injury to lungs or respiratory muscles worsen morbidity mortality.Here we use an engineering pressure loss model analyze administration medical mixtures newborns.The is used determine distribution along flow path.Numerical experiments comparing with helium, nitrous oxide, argon, xenon,...
In a previous study, in silico screening of the binding almost all proteins Protein Data Bank to each five noble gases xenon, krypton, argon, neon, and helium was reported. This massive rich data set requires analysis identify gas-protein interactions that have best strengths, those where gas occurs at site can modulate function protein, this modulation might generate clinically relevant effects. Here, we report preliminary using rational, heuristic score based on strength location. We...
Under normal conditions we continuously breathe 78% nitrogen (N2) such that the body tissues and fluids are saturated with dissolved N2. For normobaric medical gas administration at high concentrations, N2 concentration must be less than in ambient atmosphere; therefore, will begin to released by tissues. There is a need estimate time needed for denitrogenation planning of surgical procedures. In this paper describe application physiologically based pharmacokinetic model kinetics. The...
In the past decade, noble gases have emerged as highly promising neuroprotective agents. Previous studies demonstrated efficacy of argon neuroprotection in rodent models cerebral ischemia. The objective present pre-clinical study was to confirm effect a non-human primate model endovascular ischemic stroke. Thirteen adult Macaca mulatta were subjected focal ischemia induced by transient (90 min) middle artery occlusion (tMCAO). monkeys randomly allocated control group (n = 8) and an 5)....