A5002187420- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advancements in Photolithography Techniques
- DNA and Nucleic Acid Chemistry
- Nanofabrication and Lithography Techniques
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plasmonic and Surface Plasmon Research
- Advanced Surface Polishing Techniques
- Maternal and fetal healthcare
- Prenatal Screening and Diagnostics
- Integrated Circuits and Semiconductor Failure Analysis
- Pregnancy and preeclampsia studies
- Laser Design and Applications
- Electron and X-Ray Spectroscopy Techniques
- RNA modifications and cancer
- 3D IC and TSV technologies
- Photonic and Optical Devices
- Molecular Junctions and Nanostructures
- Force Microscopy Techniques and Applications
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Supramolecular Self-Assembly in Materials
- Copper Interconnects and Reliability
- Optical Coatings and Gratings
Osaka University
2016-2025
Kyoto University
2015-2024
Kansai University
2021-2024
Kawasaki City College of Nursing
2024
Osaka Health Science University
2021-2023
The University of Tokyo
1990-2023
Tottori University
2011-2023
University of Tokyo Health Sciences
2023
Osaka Women's and Children's Hospital
2020-2022
Shizuoka University
2022
Nature has developed striking light-powered proteins such as bacteriorhodopsin, which can convert light energy into conformational changes for biological functions. Such natural machines are a great source of inspiration creation their synthetic analogues. However, molecular typically operate at the nanometre scale or below. Translating controlled operation individual to larger dimension, example, 10-100 nm, features many practical applications, is highly important but remains challenging....
Abstract Self-assembly is a ubiquitous approach to the design and fabrication of novel supermolecular architectures. Here we report strategy termed ‘lipid-bilayer-assisted self-assembly’ that used assemble DNA origami nanostructures into two-dimensional lattices. structures are electrostatically adsorbed onto mica-supported zwitterionic lipid bilayer in presence divalent cations. We demonstrate bilayer-adsorbed units mobile on surface self-assembled large micrometre-sized lattices their...
Abstract Thermal annealing is usually needed to direct the assembly of multiple complementary DNA strands into desired entities. We show that, with a magnesium-free buffer containing NaCl, complex cocktails and proteins can self-assemble isothermally, at room or physiological temperature, user-defined nanostructures, such as origamis, single-stranded tile assemblies nanogrids. In situ, time-resolved observation reveals that this self-assembly thermodynamically controlled, proceeds through...
The photocatalytic oxidation reactivities of the photogenerated holes (h+) during ultraviolet or visible laser flash photolysis pure anatase and sulfur- carbon-doped TiO2 powders were investigated using time-resolved diffuse reflectance (TDR) spectroscopy. one-electron processes substrates such as methanol 4-(methylthio)phenyl (MTPM) by h+ at surface examined. TDR spectra time traces observed for charge carriers MTPM radical cation (MTPM•+) revealed that reactions generated 355-nm increased...
We herein report the real-time observation of G-quadruplex formation by monitoring G-quadruplex-induced global change two duplexes incorporated in a DNA nanoscaffold. The introduced G-rich strands formed an interstrand (3 + 1) structure presence K+, and four-stranded was disrupted removal K+. These conformational changes were visualized nanoscaffold with fast-scanning atomic force microscopy.
Heat tolerance of DNA origami structures has been improved about 30 °C by photo-cross-linking 8-methoxypsoralen. To demonstrate one its applications, the cross-linked were used for higher-temperature self-assembly, which markedly increased yield assembled product when compared to self-assembly non-cross-linked at lower-temperature. By contrast, annealing, native tiles did not self-assemble desired product; however, they formed a nonspecific broken structure.
A novel strategy for regulation of an enzymatic DNA modification reaction has been developed by employing a designed nanoscale scaffold. using enzymes often requires bending specific strands to facilitate the reaction. The methylation enzyme EcoRI methyltransferase (M.EcoRI) bends double helix 55°−59° during with flipping out second adenine in GAATTC sequence as methyl transfer proceeds. In this study, two different helical tensions, tense and relaxed states helices, were created control...
We demonstrate a novel strategy of self-assembly to scale up origami structures in two-dimensional (2D) space using multiple structures, named "2D DNA jigsaw pieces", with specially designed shape. For execution 2D along the helical axis (horizontal direction), sequence-programmed tenon and mortise were introduced promote selective connections via π-stacking interaction, sequence-complementarity, shape-complementarity. side (vertical shape-complementarity top bottom edges...
The immunostimulatory activity of phosphodiester DNA containing unmethylated cytosine-phosphate-guanine (CpG) dinucleotides, or CpG motifs, was significantly increased by the formation Y-, X-, dendrimer-like multibranched shape. These results suggest possibility that is a function structural properties branched assemblies. To elucidate relationship between them, we have designed and developed nanosized assemblies in polypod-like structures (polypod-like structured DNA, polypodna for short)...
We demonstrate a novel strategy for constructing multidirectional programmed 2D DNA nanostructures in various unique patterns by introducing photoresponsive oligonucleotides (Azo-ODNs) into hexagonal origami structures. examined regulation of assembly and disassembly reversibly different photoirradiation conditions manner. Azo-ODNs were incorporated to the structures, which then employed as self-assembly units building up nanosized architectures regulated arrangements. By adjusting numbers...
Point the finger: Zinc-finger proteins are convenient and site-selective adaptors for targeting specific locations within DNA-origami structures. Orthogonal of in structures was demonstrated by using two adaptors, application Escherichia coli lysate that contained adaptor-fused successfully afforded expected protein–DNA assembly.
CONSPECTUS: Direct imaging of molecular motions is one the most fundamental issues for elucidating physical properties individual molecules and their reaction mechanisms. Atomic force microscopy (AFM) enables direct imaging, especially biomolecules in physiological environment. Because AFM can visualize at nanometer-scale spatial resolution, a versatile observation scaffold needed precise molecule interactions reactions. The emergence DNA origami technology allows placement desired designed...
Here, we report the direct visualization of assembly/disassembly processes photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies controlled interactions between components and cholesterol moieties introduced to hexagonal structures, one whose outer edges carries Azo-ODNs. bilayer-placed dimer was disassembled into monomer units by UV irradiation, reversibly assembled again during visible light irradiation. These dynamic were...
The effect of guanidine on neuromuscular transmission was studied in the sciatic-sartorius preparation frog, using intracellular electrodes. sensitivity ned-plate to ACh measured by applying electrophoretically. Guanidine also applied same way. greatly increased amplitude e.p.p. curarized muscle without changing resting potential at end-plate region. exhibited no cholinesterase activity, and it did not change ACh. It is concluded that drug increases increasing quantity released from nerve...
Abstract We present the direct and single‐molecule visualization of in‐pathway intermediates G‐quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for structural control high‐speed atomic force microscopy (HS‐AFM) visualization, we captured images unprecedented solution‐state structures tetramolecular antiparallel (3+1)‐type intermediates, such G‐hairpin G‐triplex, with nanometer precision. No information was reported...
A light-driven artificial molecular nanomachine was constructed based on DNA scaffolding. Pyrene-modified walking strands and disulfide bond-connected stator strands, employed as anchorage sites to support walker movement, were assembled into a 2D tile. Pyrene molecules excited by photoirradiation at 350 nm induced cleavage of enabling the migrate from one cleaved next The time-dependent movement observed entire process characterized distribution walker-stator duplex four tile under...
Novel multiarm DNA structures were designed using two-dimensional origami scaffolds, and these folded into hollow three-dimensional (3D) by introducing connection strands the arms. The opening of prism was examined high-speed AFM imaging, which showed dissociation connecting arms in 3D structures.
We have demonstrated the construction of multiple porphyrin arrays in tobacco mosaic virus (TMV) supramolecular structures by self-assembly recombinant TMV coat protein (TMVCP) monomers, which Zn-coordinated (ZnP) and free-base (FbP) were site-selectively incorporated. The photophysical properties moieties incorporated assemblies also characterized. TMV-porphyrin conjugates employed as building blocks self-assembled into unique disk rod under proper conditions similar to native assemblies....