ABSTRACT Oleic acid (18:1n‐9) is the most abundant fatty in bovine adipose tissue. Because of lipid muscle contributed by intramuscular adipocytes, oleic also predominant beef. In many species, concentration tissue dictated average diet, but ruminant species such as beef cattle, hydrogenated largely to stearic ruminal microorganisms. these dependent upon activity Δ 9 desaturase, encoded stearoyl coenzyme A desaturase (SCD) gene. Expression SCD gene essential for preadipocyte differentiation,...

Circadian clocks sustain daily oscillations in gene expression, physiology, and behavior, relying on transcription–translation feedback loops of clock genes for rhythm generation. Cultured astrocytes display extracellular ATP, suggesting that ATP release is a circadian output. We hypothesized the modulates via mechanisms regulate acute from glia. To test molecular basis release, we developed methods to measure real-time <i>Bmal1::dLuc</i> reporter expression cortical astrocyte cultures mice...

Angus and Wagyu steers consuming high-roughage diets exhibit large differences in adipose tissue fatty acid composition, but there are no terminal measures of stearoyl-CoA desaturase (SCD) activity or gene expression. Also, lipids cattle fed corn-based have greater MUFA:SFA ratios than hay-based diets. We hypothesized that any changes SCD expression would precede similar lipogenesis between short- long-fed endpoints. Furthermore, production endpoints differ corn- hay-fed steers. (n = 8) were...

Postnatal muscle hypertrophy of beef cattle is the result enhanced myofibrillar protein synthesis and reduced turnover. Skeletal has been studied in fed β-adrenergic agonists (β-AA), which are receptor-mediated enhancers inhibitors degradation. Feeding β-AA to increases longissimus cross-sectional area 6% 40% compared non-treated cattle. The have reported improve live animal performance, including average daily gain, feed efficiency, hot carcass weight, dressing percentage. Treatment with...

Non-synonymous SNPs and protein coding within the promoter region of genes (regulatory SNPs) might have a significant effect on carcass traits. Imputed sequence level data 10,215 Hanwoo bulls, annotated filtered to include only regulatory (450,062 SNPs), were used in genome-wide association study (GWAS) identify loci associated with backfat thickness (BFT), weight (CWT), eye muscle area (EMA), marbling score (MS). A total 15, 176, 1 found be significantly (p &lt; 1.11 × 10−7) BFT, CWT, EMA,...

With the development of Korean economy, demand for high-quality beef, specifically Hanwoo is escalating, with marbling traits—measured by widely used score—being a key contributor to meat palatability. The differences between and lower-quality meat, according satisfaction customers, are not result from only degree but also delicacy flecks distribution. Using computer analysis technique, an index quantifying fineness 256 sirloin cuts at 12 th – 13 thoracic vertebra named F7 was developed in...

Ischemia/reperfusion (I/R) injury was induced in primary porcine cardiomyocytes a low-shear microfluidic culture chip. The chip capable of sustaining the cardiomyocyte and inducing I/R by subjecting cells to periods hypoxia lasting 3–4 hours followed normoxia. Mitochondrial membrane potential assayed using MitoTracker Red follow mitochondrial depolarization, earliest stage apoptosis. Cell adhesion morphology were also determined simultaneously with fluorescence measurements. Changes observed...

Abstract Background In this study, we assessed the accuracy of genomic prediction for carcass weight (CWT), marbling score (MS), eye muscle area (EMA) and back fat thickness (BFT) in Hanwoo cattle when using best linear unbiased (GBLUP), weighted GBLUP (wGBLUP), a BayesR model. For these models, investigated potential gain from pre-selected single nucleotide polymorphisms (SNPs) genome-wide association study (GWAS) on imputed sequence data gene expression information. We used 13,717 animals...

Angus (n = 8; 210 kg of BW) and 7/8 Wagyu 174 steers were used to evaluate the effects dietary energy source on muscle adipose tissue metabolism insulin sensitivity. Steers assigned either a grain-based (corn) or hay-based (hay) diet fed similar final BW. At slaughter, LM s.c. i.m. samples collected. Portions tissues placed immediately in liquid N for later measurement glycolytic intermediates. Fresh incubated with [U-(14)C]glucose assess glucose vitro. All vitro measures presence 0 500...

Background The expression of myogenic regulatory factors (MRFs) consisting MyoD, Myf5, myogenin (MyoG) and MRF4 characterizes various phases skeletal muscle development including myoblast proliferation, cell-cycle exit, cell fusion the maturation myotubes to form myofibers. Although it is well known that function MyoG cannot be compensated for other MRFs, molecular mechanism by which controls differentiation still unclear. Therefore, in this study, RNA-Seq technology was applied profile...

Our objectives were to evaluate the dose/payout pattern of trenbolone acetate (TBA) and estradiol-17β (E2) implants feeding zilpaterol hydrochloride (ZH) on performance carcass characteristics finishing beef steers. A randomized complete block design was used with a 3 × 2 factorial arrangement treatments. British Continental steers (n = 168; initial BW 362 kg) blocked by allotted randomly 42 pens (7 pens/treatment; 6 pens/block; 4 steers/pen). The main effects treatment implant [no (NI);...

We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous contains the greatest concentration of MUFA and lowest SFA. Therefore, we hypothesized depots would exhibit greater adipogenic gene expression over time than other major depots. Four Angus steers, each at 9, 12, 14, 16 mo age, were harvested fresh samples collected from brisket, chuck, rib, loin, sirloin, round, flank, plate. Relative for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β),...

British × Continental steers (n = 168; 7 pens/treatment; initial BW 362 kg) were used to evaluate the effect of dose/payout pattern trenbolone acetate (TBA) and estradiol-17β (E2) feeding zilpaterol hydrochloride (ZH) on serum urea-N (SUN), NEFA, IGF-I, E2 concentrations LM mRNA expression estrogen (ER), androgen (ANR), IGF-I (IGF-IR), β1-adrenergic (β1-AR), β2-adrenergic (β2-AR) receptors IGF-I. A randomized complete block design was with a 3 2 factorial arrangement treatments. Main effects...

We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells a co-culture system. Herein we hypothesize saturated fatty acids would adipogenic/lipogenic expression, whereas mono- and polyunsaturated have the opposite effect. Bovine semimembranosus (BSC) intramuscular (IPA) were isolated from crossbred steers cultured with 10% fetal serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) 1% antibiotics during 3-d proliferation...

We previously showed that a combined trenbolone acetate (TBA)/estradiol-17β (E2) implant significantly increases IGF-I mRNA levels in the LM of feedlot steers by 28 d after implantation. Here we compare effects E2 (25.7 mg), TBA (120 and mg)/E2 (24 mg) implants on IGF-I, receptor (IGFR-1), estrogen (ER)-α androgen (AR) steers. Twenty yearling crossbred with an average initial BW 421.1 ± 3.6 kg were stratified randomly assigned to 1 4 treatments: 1) nonimplanted, control; 2) implanted E2; 3)...

We evaluated growth-related responses to ractopamine in steers and heifers. Sixteen Angus (512 kg) 16 heifers (473 housed individual pens were used a complete block design. At 90 97 d before the experiment, implanted with 120 mg of trenbolone acetate 24 estradiol-17beta (Component TE-S) 140 14 TE-H). Treatments arranged as 2 x factorial included sex (steer vs. heifer) ractopamine-HCl (0 or 200 mg/d). Cattle fed diet based on steam-flaked corn once daily. Blood LM biceps femoris (BF) biopsy...

Four experiments evaluated the effect of implant dose and release pattern on performance carcass traits crossbred beef steers. In Exp. 1, steers (4 to 7 pens/treatment; initial BW = 315 kg) were fed an average 174 d. Treatments 1) no (NI); 2) Revalor-S [120 mg trenbolone acetate (TBA) 24 estradiol 17β (E(2)); REV-S]; 3) Revalor-IS followed by REV-S (cumulatively 200 TBA 40 E(2); reimplanted at 68 74 d; REV-IS/S); 4) Revalor-XS (200 REV-X). Carcass-adjusted final was greater (P < 0.05) for...