A5010283472- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Fluorescence Microscopy Techniques
- AI in cancer detection
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- CAR-T cell therapy research
- Neuroscience and Neural Engineering
- SARS-CoV-2 and COVID-19 Research
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Mechanisms of cancer metastasis
- Renal and related cancers
- Nanowire Synthesis and Applications
- Wnt/β-catenin signaling in development and cancer
- Planarian Biology and Electrostimulation
- Diabetes and associated disorders
- Digital Imaging for Blood Diseases
- Plant and Biological Electrophysiology Studies
- Slime Mold and Myxomycetes Research
University of Freiburg
2010-2023
University Medical Center Freiburg
2015
Abstract B lymphocytes recognize bacterial or viral antigens via different classes of the cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces, and are thought to perform sensory functions in screening antigen‐bearing surfaces. Here, we have used lattice light‐sheet microscopy combination with tailored custom‐built 4D image analysis study cell‐surface topography cells Ramos Burkitt's Lymphoma line spatiotemporal organization IgM‐BCR. B‐cell surfaces...
It has long been known that electrical fields (EFs) are able to influence the direction of migrating cells, a process commonly referred as electrotaxis or galvanotaxis. Most studies have focused on cells equipped with an existing polarity before EF application, making it difficult delineate EF-specific pathways. Here we study initial events in front–rear organization spreading keratinocytes dissect molecular requirements for random and EF-controlled polarization. We find Arp2/3-dependent...
The major function of B lymphocytes is to sense antigens and produce protective antibodies after activation. This requires the expression a B-cell antigen receptor (BCR), evolutionary conserved mechanisms seem exist that ensure cells without BCR do not develop nor survive in periphery. Here, we show loss on Burkitt lymphoma leads decreased mitochondrial impaired metabolic flexibility. Strikingly, this phenotype does result from absence classical Syk-dependent signal but rather compromised ER...
Abstract B lymphocytes recognize bacterial or viral antigens via different classes of the cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces and are thought to perform sensory functions in screening antigen-bearing surfaces. Here, we have studied surface features Ramos cells spatiotemporal organization IgM-BCR using lattice light sheet microscopy combination with tailored custom-built 4D image analysis. were found form dynamic networks elevated...
The B-cell antigen receptor (BCR) is composed of a membrane-bound immunoglobulin (mIg) class M, D, G, A or E for recognition and disulfide-linked heterodimer between Igα Igβ (Igα/β, also known as CD79A CD79B) that functions the signalling entity. organizing principle BCR assembly remains elusive. Here we report cryo-electron microscopy structures intact IgM at 8.2 Å resolution its Fab-deleted form (IgM BCRΔFab) 3.6 resolution. At ectodomain (ECD), position their respective Ig folds roughly...
In this paper, we present a novel framework for the modeling of cell-migration, and more specifically migration human keratinocytes. The model decouples embodiment an artificial cell into two elements. A cell-body is implemented by sets springs forming membrane supporting cortical-cytoskeleton, which allows rigidity flexibility. leading-edge, structure spreading around cell-body, simulated with stochastic cellular-automata. It defines migratory forces that pull according to its local spread...
Abstract SARS-CoV-2, the causative agent of Covid-19, is known to evade immune system by several mechanisms. This includes shutdown host cellular protein synthesis, which abrogates induction antiviral interferon responses. The virus initiates infection susceptible cells binding with its spike (S) angiotensin-converting enzyme 2 (ACE2). Here we applied T cell receptor fusion construct (TRuC) technology engineer against such infected cells. In our TRuCs an S-binding domain fused CD3ε component...