Abstract DNA methylation is essential for epigenetic regulation of gene transcription and development in many animals, plants fungi. We investigated whether plays a role the secondary metabolism Aspergillus flavus , identified DmtA methyltransferase from A. produced dmtA knock-out mutant by replacing coding sequence with pyrG selectable marker. The null lines white fluffy mycelium liquid medium displayed slightly flavescent conidial pigmentation compared normal yellow wild-type strain when...

Abstract To investigate the changes in transcript and relative protein levels response to temperature, complementary transcriptomic proteomic analyses were used identify Aspergillus flavus grown at 28 °C 37 °C. A total of 3,886 proteins identified 2,832 reliably quantified. subset 664 was differentially expressed upon temperature enriched several Kyoto Encyclopedia Genes Genomes pathways: translation-related pathways, metabolic pathways biosynthesis secondary metabolites. The profiles showed...

Abstract Background Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are effective methods treatment thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) a promising agent. DFE was isolated from , typical and popular soybean-fermented food China, it can dissolve fibrin directly efficiently. A strain, Bacillus subtilis LD-8547 produced high activity has been our lab previously. Results In study, effect studied vitro vivo...

Histone acetyltransferases (HATs) help regulate fungal development and the production of secondary metabolites. In this study, we determined that HAT AflGcnE influenced morphogenesis aflatoxin biosynthesis in Aspergillus flavus. We observed localized to nucleus cytoplasm during conidial germination stages, while it was located mainly hyphal stage. Deletion AflgcnE inhibited growth A. flavus decreased hydrophobicity cell surface. The ΔAflgcnE mutant exhibited a lack asexual sporulation unable...

Aspergillus flavus produces a variety of toxic secondary metabolites; among them, the aflatoxins (AFs) are most well known. These compounds highly mutagenic and carcinogenic, particularly AFB₁. A. is capable colonizing number economically-important crops, such as corn, cotton, peanut tree nuts, contaminating them with AFs. Molecular genetic studies in could identify novel gene targets for use strategies to reduce AF contamination its adverse impact on food feed supplies worldwide. In current...

In Aspergillus nidulans, the nitrogen metabolite repression regulator NmrA plays a major role in regulating activity of GATA transcription factor AreA during metabolism. However, function nmrA flavus has not been previously studied. Here, we report identification and functional analysis A. flavus. Our work showed that amino acid sequences are highly conserved among species protein contains canonical Rossmann fold motif. Deletion slowed growth but significantly increased conidiation sclerotia...

As the main producer of aflatoxins, Aspergillus flavus is also one most important causes invasive and non-invasive aspergillosis. Therefore, it crucial to unravel regulatory mechanisms growth, metabolism, pathogenicity A. flavus. SWD1 highly conserved across species for maintaining COMPASS methyltransferase activity, but bio-function in has not been explored. Through genetic analysis, this study revealed that involved fungal morphogenesis AFB1 biosynthesis by regulating orthodox pathways...

Aspergillus flavus produces mycotoxins especially aflatoxin B1 and infects crops worldwide. As a PHD transcription factor, there is no report on the role of Rum1 in virulence spp. yet. This study explored biological function A. through construction rum1 deletion mutants complementation strains with method homologous recombination. It was found, study, that negatively regulates conidiation abaA brlA, positively sclerotia formation nsdC, nsdD, sclR, triggers synthesis, enhances activity...

The filament fungal pathogen, Aspergillus flavus, spreads worldwide and contaminates several important crops. Histone posttranslational modifications are deeply involved in development virulence, but the biological function of histone methyltransferase AflSet1 A. flavus is still unknown. In study, Aflset1 deletion strain was constructed through homologous recombination, it found that up-regulates hyphae growth, promotes conidiation by sporulation regulation genes: abaA brlA. It also involves...

Aspergillus flavus infects various crops with aflatoxins, and leads to aspergillosis opportunistically. Though H3K36 methylation plays an important role in fungal toxin metabolism virulence, no data about the biological function of A. virulence has been reported. Our study showed that Set2 histone methyltransferase family, AshA SetB, involves morphogenesis mycotoxin anabolism by regulating related transcriptional factors, they are for animals. Western-blotting double deletion analysis...

T-2 toxin is known to induce apoptosis in mammalian cells. The mechanism of induced by has been proposed be linked with oxidative stress and mitochondrial pathway. In the current study, toxic effect on Hela, Bel-7402, Chang liver cells was examined dose-dependent time-dependent manner MTT assay. Caspase-3 found up-regulated under stress, which suggested that cell apoptosis. Endogenous GSH MDA levels all three lines were down- respectively, indicated link between intracellular stress. It also...

This study focused on AflSkn7, which is a stress response regulator in the aflatoxin-producing Aspergillus flavus. The ΔAflSkn7 mutants exhibited partially defective conidial formation and complete inability to generate sclerotia, indicating AflSkn7 affects A. flavus asexual sexual development. tolerated osmotic but were susceptible effects of cell wall stress. Additionally, especially sensitive oxidative These observations confirmed that influences responses rather than responses. was...

Reversible protein phosphorylation is known to play important roles in the regulation of various cellular processes eukaryotes. Phosphatases-medicated dephosphorylation are integral components signal pathways by counteracting action kinases. In this study, we characterized functions CDC14, a dual-specificity phosphatase development, septation, secondary metabolism and crop infection Aspergillus flavus. Deletion AflCDC14 resulted nutrient-dependent growth defect abnormal conidium morphology....

Aflatoxins (AFs) are a group of highly oxygenated polyketidese-derived toxins mainly produced by Aspergillus flavus and A. parasiticus, whose biosynthesis mechanisms extremely sophisticated. Methylation is known as the major form epigenetic regulation, which correlated with gene expression. As DNA methylation inhibitor 5-azacytidine (5-AC) blocks AF production, we studied AFB1 metabolism morphological changes treatment 5-AC in liquid culture. The results show that caused decrease production...

The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis in Aspergillus flavus . However, the underlying molecular mechanism is still unclear. In this study, by gene deletion complementation, we found essential for mycelia conidial production, sclerotia formation, aflatoxin synthesis, host colonization. Chromatin immunoprecipitation sequencing (ChIP-seq) RNA (RNA-seq) analysis revealed that played key roles...

Aspergillus flavus and its secondary metabolites aflatoxins pose a significant threat to the health of humans, animals, plants. Therefore, there is an urgent need control A. contamination. AfverB plays key role in aflatoxin gene cluster; however, function mechanism fungal development virulence remain poorly understood. In this study, we constructed afVerB deletion mutants (∆afVerB−1 ∆afVerB−2) two CYP domain (afVerB∆D1 afVerB∆D2) through homologous recombination. Phenotype analysis revealed...

As an opportunistic pathogen, Aspergillus flavus is one of the major causes food contamination around world. In this study, pbsB gene knockout mutant (ΔpbsB) and overexpression strain (OE) A. were constructed by homologous recombination. The results showed that mycelia growth, conidiation formation sclerotia in ΔpbsB significantly suppressed, up-regulated OE strian compared to wild-type (WT). Q-PCR analysis PbsB regulated through related nsdC. With TLC qRT-PCR analysis, it was found...

Abstract Aspergillus flavus and its main secondary metabolite AFB1 pose a serious threat to several important crops worldwide. Recently, it has been reported that some PHD family transcription factors are involved in the morphogenesis biological synthesis A. , but role of Cti6, domain containing protein is totally unknown. The study was designed reveal function Cti6 fungus by deletion cti6 two domains (PHD Atrophin-1) through homologous recombination, respectively. results showed might...

RGS proteins, as crucial regulators of the G protein signaling pathway, are widely distributed in fungi, while little is known about their roles Aspergillus flavus development and aflatoxin. In this study, we identified six proteins A. revealed that these have important functions regulation conidia, sclerotia, aflatoxin formation.