A5061526145- Toxin Mechanisms and Immunotoxins
- HIV Research and Treatment
- Cystic Fibrosis Research Advances
- Microbial Metabolic Engineering and Bioproduction
- Transgenic Plants and Applications
- Microbial Natural Products and Biosynthesis
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Alcohol Consumption and Health Effects
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- T-cell and B-cell Immunology
- Helicobacter pylori-related gastroenterology studies
- HIV-related health complications and treatments
- Neonatal Respiratory Health Research
- Diabetes and associated disorders
- Pneumonia and Respiratory Infections
- Immune responses and vaccinations
- Algal biology and biofuel production
- Antibiotic Resistance in Bacteria
- Aquatic Ecosystems and Phytoplankton Dynamics
- Endoplasmic Reticulum Stress and Disease
- Digestive system and related health
- Metabolism, Diabetes, and Cancer
Tulane University
2019-2025
Tianjin University of Science and Technology
2025
Institute of Process Engineering
2021-2024
University of Chinese Academy of Sciences
2023-2024
Chinese Academy of Sciences
2024
Children's Hospital of New Orleans
2008-2022
Tula University
2019-2020
Tianjin University
2016-2017
Louisiana State University Health Sciences Center New Orleans
2003-2017
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2016
Abstract Previous studies have shown that T cells from human alcoholics overexpress activation or memory markers such as leukocyte antigen-DR, CD45RO, CD57, and CD11b may reduced levels of CD62L. In those studies, we demonstrated the increased CD57+ cell population rapidly produces interferon-γ (IFN-γ) tumor necrosis factor α, independent a second signal requirement, consistent with an effector population. contrast to length alcohol abuse by alcoholics, most work mice has involved 2-week...
Optimal microbicidal activity of human polymorphonuclear leukocytes (PMN) relies on the generation toxic agents such as hypochlorous acid (HOCl) in phagosomes. HOCl formation requires H2O2 produced by NADPH oxidase, myeloperoxidase derived from azurophilic granules, and chloride ion. Chloride transport cytoplasm into phagosomes channels which include cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated channel. However, phagosomal targeting CFTR PMN has not been...
A subunit mucosal vaccine conferred protection against heterologous Klebsiella pneumoniae strains, via IL-17R signaling in lung fibroblasts.
Cystic fibrosis (CF) is a common and deadly inherited disease, caused by mutations in the CFTR gene that encodes cAMP-activated chloride channel. One outstanding manifestation of disease persistent bacterial infection inflammation lung, which claims over 90% CF mortality. It has been debated whether neutrophil-mediated phagocytic innate immunity any intrinsic defect contributes to host lung defense failure. Here we compared phagosomal targeting, hypochlorous acid (HOCl) production, microbial...
Male mice lacking the androgen receptor (AR) in pancreatic β cells exhibit blunted glucose-stimulated insulin secretion (GSIS), leading to hyperglycemia. Testosterone activates an extranuclear AR amplify glucagon-like peptide-1 (GLP-1) insulinotropic action. Here, we examined architecture of targets that regulate GLP-1 action male cells. cooperates with enhance cAMP production at plasma membrane and endosomes via: (1) increased mitochondrial CO2, activating HCO3−-sensitive soluble adenylate...
Magnetite nanoparticle coated silica (Fe3O4@SiO2) hybrid nanomaterials hold an important position in the fields of cell imaging and drug delivery. Here we report a large scale synthetic procedure that allows attachment magnetite nanoparticles onto surface situ. Many different such as Stöber nanospheres, mesoporous nanoparticles, hollow nanotubes have been with high density layer water-dispersible nanoparticles. The size efficiency can be well tuned by adjusting precursor concentration reflux...
Abstract Alcohol abuse has long been known to adversely affect innate immune responses and predispose infections. One cellular mechanism responsible for this effect is alcohol-induced suppression of TNF-α by mononuclear phagocytes. We undertook experiments better understand the mechanisms which alcohol dose-dependently suppresses TNF elaboration human monocytes. Here we show in primary monocytes cell lines that LPS-induced secretion post-transcriptionally inhibiting processing...
Background Mechanisms of antibody-mediated neutralization are much interest. For plant and bacterial A-B toxins, A chain mediates toxicity B binds target cells. It is generally accepted taught that antibody (Ab) neutralizes by preventing toxin binding to Yet for some ricin included, anti-A Abs afford greater protection than anti-B. The mechanism(s) whereby the neutralize toxins not understood. Methodology/Principal Findings We use quantitative confocal imaging, assays, other techniques study...
Abstract Lung tissue resident memory (TRM) cells are thought to play crucial roles in lung host defense. We have recently shown that immunization with the adjuvant LTA1 (derived from A1 domain of E. coli heat labile toxin) admixed OmpX K. pneumoniae can elicit antigen specific Th17 TRM provide serotype independent immunity members Enterobacteriaceae family. However, upstream requirements generate these unclear. Single-cell RNA-seq showed vaccine-elicited expressed high levels IL-1R1,...
Alternative oxidase (AOX) is a terminal in the mitochondrial electron transport chain that does not contribute to generation of ATP. It plays critical role maintaining balance between reactive oxygen species (ROS) production and intracellular redox homeostasis within mitochondria. In study, overexpression knockdown approaches were employed investigate function AOX. AOX-silenced strains (AOXi3 AOXi25) AOX-overexpressed (OE-AOX2 OE-AOX21) constructed. The ROS level transcription...
BACKGROUND: Dysregulation of TRPV4 (transient receptor potential vanilloid 4)–mediated signaling has been associated with inflammation and tissue fibrosis, both which are key features in the pathophysiology lymphatic system diseases; however, expression functional roles channels remain largely unexplored. METHODS: We generated a single-cell RNA sequencing dataset from microdissected mouse collecting vessels to characterize Trpv4 . Using novel fx/fx strain Cre-lines Prox1 - CreER T2 LysM-Cre...
Skeletal muscle stem cells play a critical role in regeneration of myofibers. We previously demonstrated that chronic binge alcohol (CBA) markedly attenuates myoblast differentiation potential and myogenic gene expression. Muscle-specific microRNAs (miRs) are implicated regulation genes. The aim this study was to determine whether myoblasts isolated from asymptomatic CBA-administered simian immunodeficiency virus (SIV)-infected macaques treated with antiretroviral therapy (ART) showed...
Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis required to further explore its biosynthetic network determine key limiting steps for rationally engineering. To achieve this goal, an engineering approach guided by model was implemented better understand ascomycin biosynthesis improve production.The conservation of Streptomyces species first investigated comparing...
ABSTRACT The envelope (Env) glycoprotein of HIV is the only intact viral protein expressed on surface both virions and infected cells. Env target neutralizing antibodies (Abs) has been subject intense study in efforts to produce vaccines. Therapeutic anti-Env Abs can also exert antiviral effects via Fc-mediated effector mechanisms or as cytotoxic immunoconjugates, such immunotoxins (ITs). In course screening monoclonal (MAbs) for their ability deliver agents Env-transfected cells, we noted...
The manifestation of virulence traits in Cryptococcus neoformans is thought to rely on intracellular transport, a process not fully explored this pathogenic fungus. Through interaction cloning, we identified multi-modular protein, Cin1 (cryptococcal intersectin 1), whose domain structure similar that the human endocytic protein ITSN1. contains an N-terminal EH domain, central coiled-coil region, WH2 two SH3 domains and C-terminal RhoGEF (DH)-PH domain. Interestingly, alternative mRNA...
We have studied the role of antibody (Ab) Fc region in mediating protection from ricin toxicity. compared vitro and vivo effects intact Ig Fab fragments derived two different neutralizing Ab preparations, one monoclonal, other polyclonal. Consistent results were obtained each, showing little difference between terms antigen binding neutralization, but with relatively large differences animals. also whether importing into cell by receptors enhanced intracellular neutralization toxin. found...
Abstract Cystic fibrosis is a life-shortening genetic disorder, caused by mutations in the gene that encodes cystic transmembrane-conductance regulator, cAMP-activated chloride and bicarbonate channel. Persistent neutrophilic inflammation major contributor to lung disease. However, how regulator loss of function leads excessive its clinical sequela remains incompletely understood. In this study, neutrophils from F508del-CF healthy control participants were compared for transcription. We...
In this study, the functional role of INSM1 is examined with an AR42J acinar cell model for trans-differentiation into insulin-positive cells. Islet transcription factors (ITFs: INSM1, Pdx-1, and NeuroD1) are over-expressed in cells using adenoviral vectors. Addition Ad-INSM1 alone or combination three ITFs to triggers cellular trans-differentiation. Ectopic expression directly induces insulin, Pax6, Nkx6.1 expression, whereas Pdx-1 NeuroD1 were slightly suppressed by INSM1. further enhances...