A5046708550- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Hydrogels: synthesis, properties, applications
- Cancer Cells and Metastasis
- Supramolecular Self-Assembly in Materials
- Foreign Language Teaching Methods
- Neuroblastoma Research and Treatments
- Oral Health Pathology and Treatment
- dental development and anomalies
- Educational Innovations and Challenges
- Periodontal Regeneration and Treatments
- Silk-based biomaterials and applications
- Educational Methods and Teacher Development
- Glioma Diagnosis and Treatment
- Wound Healing and Treatments
- Oral and Maxillofacial Pathology
Universidad de Granada
2020-2024
Instituto de Investigación Biosanitaria de Granada
2021-2024
The inclusion of magnetic nanoparticles (MNP) in a hydrogel matrix to produce hydrogels has broadened the scope these materials biomedical research. Embedded MNP offer possibility modulate physical properties remotely and on demand by applying an external field. Moreover, they enable permanent changes mechanical hydrogel, as well alterations micro- macroporosity its three-dimensional (3D) structure, with associated potential induce anisotropy. In this work, behavior biocompatible...
Abstract Advances in skin tissue engineering have promoted the development of artificial substitutes to treat large burns and other major loss conditions. However, one main drawbacks bioengineered is need obtain a amount viable epithelial cells short periods time, making biofabrication process challenging slow. Enhancing cell cultures by using mesenchymal stem secretome can favor scalability manufacturing processes for skin. The effects three different types derived from human cells, e.g....
The aim of this study was to generate novel models bioartificial human oral mucosa with increased vascularization potential for future use as an advanced therapies medicinal product, by using different vascular and mesenchymal stem cell sources.Oral substitutes could contribute the clinical treatment complex diseases affecting cavity. Although several artificial have been described, biointegration is a major issue that be favored generation once grafted in vivo.Three types cells (MSCs) were...
Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of most promising mesenchymal (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC fully differentiated tissues, to preserve their unique features. basement membrane (BM) an essential structure during embryonic development adult tissues. Epithelial BMs well-known, but similar structures present other histological structures, such as peripheral nerve fibers, myocytes...
Purpose: We carried out a histological characterization analysis of the stromal layer human heterotypic cornea substitutes generated with extra-corneal cells to determine their putative usefulness in tissue engineering. Methods: Human bioartificial corneas were using nanostructured fibrin-agarose biomaterials corneal immersed within. To generate heterotypical corneas, umbilical cord Wharton's jelly stem (HWJSC) cultured on surface obtain an epithelial-like layer. These compared control...
Recent advances in tissue engineering offer innovative clinical alternatives dentistry and regenerative medicine. Tissue combines human cells with compatible biomaterials to induce regeneration. Shortening the fabrication time of used will contribute treatment improvement, biomaterial functionalization can be exploited enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including oral mucosa explants within for generation bioengineered...
The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. aim work to characterize hUC at situ ex vivo levels stablish their potential use vascular regeneration. Human cords were obtained histologically prepared for analysis four (intervascular-IV, perivascular-PV, subaminoblastic-SAM, Wharton's jelly-WH), primary cell cultures mesenchymal stem cells (hUC-MSC) isolated from each region obtained. results confirmed...
Purpose: Obtaining sufficient numbers of cells in a short time is major goal cell culturing therapy and tissue engineering. However, current bidimensional (2D) culture methods are associated to several limitations, including low efficiency the loss key differentiation markers on cultured cells. Methods: In present work, we have designed novel biofabrication method based three-dimensional (3D) system (FIBRIAGAR-3D). Human Wharton’s jelly mesenchymal stromal (HWJSC) were 3D using 100%, 75%,...
Human artificial corneas (HAC) generated by tissue engineering recently demonstrated clinical usefulness in the management of complex corneal diseases. However, biological mechanisms associated to their regenerative potential need be elucidated. In present work, we HAC using nanostructured fibrin-agarose biomaterials with cultured epithelial and stromal cells, compared structure histochemical immunohistochemical profiles control native (CTR-C) limbus (CTR-L) determine level biomimicry these...
Critical defects of the mandibular bone are very difficult to manage with currently available materials and technology. In present work, we generated acellular cellular substitutes for human by tissue engineering using nanostructured fibrin-agarose biomaterials, without adipose-tissue-derived mesenchymal stem cells differentiated osteogenic lineage inductive media. Then, these were evaluated in an immunodeficient animal model severely critical damage order assess potential bioartificial...
Abstract Background Histology of human oral mucosa is closely related with its function and anatomical location, a proper characterization the masticatory could be very useful in periodontal pathology. Objective In present work, we have carried out comprehensive study order to determine main histological features parakeratinized (POM) orthokeratinized (OOM) using light electron microscopy. Methods To perform this, used several histological, histochemical immunohistochemical methods detect...
Neural Invasion (NI) is a key pathological feature of cancer in the colonization distant tissues, and its underlying biological mechanisms are still scarcely known. The complex interactions between nerve tumor cells, along with stroma, make it difficult to reproduce this pathology effective study models, which turn has limited understanding NI pathogenesis. In study, we have designed three-dimensional model squamous cell carcinoma combining human epidermoid cells (hECCs) complete peripheral...
In the article affective-motivational components for science learning in pharmacy students are identified because process is conditioned by these components. The study was carried out using a specific questionnaire. results showed variations self-determination, self-efficacy, intrinsic motivation, and short long-term with differences between both genders.