A5100774272- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Genetic Syndromes and Imprinting
- Privacy-Preserving Technologies in Data
- Genetics and Neurodevelopmental Disorders
- Renal and related cancers
- Neonatal Respiratory Health Research
- Genetic and phenotypic traits in livestock
- Reproductive Biology and Fertility
- Protein Degradation and Inhibitors
- Big Data and Digital Economy
- Ethics in Clinical Research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Urologic and reproductive health conditions
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Tracheal and airway disorders
Xi'an Jiaotong University
2024
National Engineering Research Center for Information Technology in Agriculture
2024
International Peace Maternity & Child Health Hospital
2020-2023
Shanghai Jiao Tong University
2020-2021
Abstract Rare coding variants have been proven to be one of the significant factors contributing spermatogenic failure in patients with non-obstructive azoospermia (NOA) and severe oligospermia (SO). To delineate molecular characteristics idiopathic NOA SO, we performed whole-exome sequencing 314 unrelated Chinese Han origin verified our findings by comparing 400 fertile controls. We detected six pathogenic/likely pathogenic four unknown significance, genes known cause NOA/SO, 9 which had...
Abstract During human spermatogenesis, germ cells undergo dynamic changes in chromatin organization/re-packaging and transcriptomes. In order to better understand the underlying mechanism(s), scATAC-Seq of 5376 testicular from 3 normal men were performed. Data analyzed parallel with scRNA-Seq data cells. all, 10 cell types associated spermatogenesis 6 somatic identified, along 142 024 peaks located promoter, genebody CpG Island. We had examined accessibility all chromosomes, chromosomes 19...
A substantial number of male infertility is caused by azoospermia. However, the underlying etiology and molecular basis remain largely unknown. Through single-cell (sc)RNA sequencing, we had analyzed testis biopsy samples from two patients with obstructive azoospermia (OA) nonobstructive (NOA). We found only somatic cells in NOA explored transcriptional changes Sertoli response to a loss interactions germ cells. Moreover, observed cell population discrepancy between an OA (postvasectomy)...
The intricate interaction between spermatogonial stem cell (SSC) and testicular niche is essential for maintaining SSC homeostasis; however, this remains largely uncharacterized. In study, to characterize the underlying signaling pathways related paracrine factors, we delineated intercellular interactions in both adult mice humans under physiological conditions dissected niche-derived regulation of maintenance recovery conditions, thus uncovering role C-C motif chemokine ligand 24...
Seminal plasma contains a high concentration of extracellular vesicles (EVs). The heterogeneity small EVs or the presence nonvesicular matter (NV) pose major obstacles in understanding composition and function seminal EVs. In this study, we employed high-resolution density gradient fractionation to accurately characterize NV. We found that could be divided into 3 different subtypes-namely, high-density EV (EV-H), medium-density (EV-M), low-density (EV-L)-after purification using iodixanol,...
Long non-coding (lnc) RNAs are a series of longer than 200 nucleotides that do not code for protein products. Whole-genome expression profiles lncRNAs suggest they play important roles in spermatogenesis because particularly abundant testes. However, most their characteristics and functions remain unclear. The aim this study was to define the function lncRNA5512, which is spermatocytes round spermatids, mouse fertility invivo. To investigate we generated lncRNA5512-knockout mice by clustered...
As a BET bromodomain inhibitor, JQ1 has been proven have efficacy against number of different cancers. In terms male reproduction, may be used as new type contraceptive, since treatment in mice could lead to germ cell defects and decrease sperm motility, moreover, this effect is reversible. However, the mechanism acting on gene regulation spermatogenesis remains unclear. Here, we performed single-cell RNA sequencing (scRNA-seq) mouse testes treated with or vehicle control determine...
In mammals, DNA methylation patterns are established by various types of methyltransferases and can be stably passed on during cell division, thus creating a paradigm for epigenetic regulation that mediate long-lasting changes in gene expression even when the initial triggering signal has disappeared. Although functional deficiency DNMT3A, one methyltransferases, leads to abnormal result developmental deficits impacts its overexpression tissue remain unclear. Here, our previously hDNMT3A...
In the era of big data, widespread adoption Internet applications and information services has resulted in extensive collection individuals' sensitive biological information, increasing risk privacy breaches.Event cameras, as novel bio-inspired sensors, exhibit characteristics such low latency, high dynamics, texture independence.They offer a fresh technological approach to addressing protection issues on data side, making them suitable for private like home monitoring.This study thoroughly...