A5091513274- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- Reproductive System and Pregnancy
- Renal and related cancers
- Extracellular vesicles in disease
- RNA modifications and cancer
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Ovarian function and disorders
- Microtubule and mitosis dynamics
- Hormonal and reproductive studies
- Birth, Development, and Health
- Effects and risks of endocrine disrupting chemicals
- Nanocluster Synthesis and Applications
- Catalytic C–H Functionalization Methods
- Prenatal Screening and Diagnostics
- Iron Metabolism and Disorders
Sir Run Run Shaw Hospital
2021-2025
Zhejiang University
2021-2025
Nantong University
2018-2025
Ningxia Medical University
2021-2025
Michigan State University
2020-2025
Hangzhou First People's Hospital
2025
Westlake University
2025
Brown University
2025
Rhode Island Hospital
2025
Affiliated Hospital of Nantong University
2022-2024
Many members of the TGF-beta superfamily are indicated to play important roles in ovarian follicular development, such as affecting granulosa cell function and oocyte maturation. Abnormalities associated with TGF-beta1 signaling transduction could result female infertility. MicroRNAs (miRNAs), small noncoding RNAs, were recently found regulate gene expression at posttranscriptional levels. However, little is known about role miRNAs TGF-beta-mediated proliferation function. In this study,...
Abstract Background MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function miRNAs human testes involved infertility. Methods In this study, miRNA profiles patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies. Results Altered microRNA NOA was found, 154 differentially down-regulated 19 up-regulated...
The experiments compare intracellular changes in porcine eggs induced by electrical activation with those sperm penetration. Adequate electrostimulation induces both cortical granule exocytosis and protein synthesis similar to during fertilization. However, ionic differ markedly from initiated at Thus, dynamic video imaging using Fura-2 as a Ca2+ probe provides evidence that parthenogenetic is single sharp rise the concentration of free calcium ([Ca2+]i) egg. transient increase triggered an...
It has been recently ascribed to several inflammatory cytokines (i.e. TGF-β3, TNF-α and IL-1) a functional role in regulating Sertoli cell blood-testis barrier (BTB) dynamics. In the testis, IL-6 inhibits meiotic DNA synthesis during seminiferous epithelium cycle, reduces sperm motility influences secretion of transferrin inhibin B by cells. Also, it shown that affects tight junction permeability cells, but, little is known about its BTB. The aim this study was investigate molecular...
Our previous studies have shown that microRNA-383 (miR-383) is one of the most down-regulated miRNA in TGF-β1-treated mouse ovarian granulosa cells (GC). However, roles and mechanisms miR-383 GC function during follicular development remain unknown. In this study, we found was mainly expressed oocytes follicles. Overexpression enhanced estradiol release from through targeting RNA binding motif, single stranded interacting protein 1 (RBMS1). inhibited RBMS1 by affecting its mRNA stability,...
Sperm morphology mainly refers to the shape of head, length flagellar segments, including midpiece, principal piece and end piece, size accessory structures, axonemes, outer dense fibers (ODFs), mitochondrial sheath (MS) fibrous (FS). Across species, there is considerable diversity in morphology. An established theory posits that sperm flagellum, especially a critical factor influencing metabolism velocity. However, our understanding relationships between ultrastructures incomplete. The...
Abstract Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role the protection of sperm tail against shear forces. However, and relevant mechanisms ODFs modulating motility its pathological involvement asthenozoospermia were unknown. Here, we found that percentage ODF defects was higher asthenozoospermic samples than control significantly correlated with axoneme non‐motile sperm. Furthermore, expression levels major components (Odf1, 2, 3,...
Two percent of men are infertile owing to defects in sperm production. In 10-15% cases, Y chromosome deletions that encompass critical spermatogenesis genes detected; the remaining cause infertility is unknown. model organisms, recombination infertility, germ cell aneuploidy and subsequent development inviable or abnormal progeny. Several studies have also linked higher rates women. Thus, we reasoned defective may be a major with poor no production performed first comparison parameters...
Abstract Tamoxifen represents a major adjuvant therapy to those patients with estrogen receptor-alpha positive breast cancer. However, tamoxifen resistance occurs quite often, either de novo or acquired during treatment. To investigate the role of miR-320a in development tamoxifen, we established tamoxifen-resistant (TamR) models by continually exposing MCF-7 T47D cancer cells and identified microRNA(miRNA)-320a as down-regulated miRNA resistant cells. Re-expression was sufficient sensitize...
Many long noncoding RNA (lncRNA) species have been identified in gametes. However, the biogenesis and function of other categories lncRNAs gametes is poorly understood. Here, we profiled expression mRNAs spermatogonial stem cells (SSC), type A spermatogonia (A), pachytene spermatocytes (PS) round spermatids (RS) by microarray analysis. We analyze total lncRNA/mRNA these four germ found that maximum number (22127) minimum PS (14456). Also, (19923) (13941). Furthermore, trend specific was...
Abstract Long non-coding RNAs (lncRNAs), which are extensively transcribed from the genome, have been proposed to be key regulators of diverse biological processes. However, little is known about role lncRNAs in regulating spermatogenesis human males. Here, using microarray technology, we show altered expression testes infertile men with maturation arrest (MA) or hypospermatogenesis (Hypo), 757 and 2370 differentially down-regulated 475 163 up-regulated MA Hypo, respectively. These findings...
A ligand-controlled iridium-catalyzed semihydrogenation of alkynes to E- and Z-alkenes with ethanol was developed. Effective selectivity control achieved by ligand regulation. The use 1,2-bis(diphenylphosphino)ethane (DPPE) 1,5-cyclooctadiene (COD) critical for the stereoselective alkynes. general applicability this procedure highlighted synthesis more than 40 alkenes, good stereoselectivities. value our approach in practical applications investigated studying effects pinosylvin...
Abstract Rare coding variants have been proven to be one of the significant factors contributing spermatogenic failure in patients with non-obstructive azoospermia (NOA) and severe oligospermia (SO). To delineate molecular characteristics idiopathic NOA SO, we performed whole-exome sequencing 314 unrelated Chinese Han origin verified our findings by comparing 400 fertile controls. We detected six pathogenic/likely pathogenic four unknown significance, genes known cause NOA/SO, 9 which had...
Paternal life experiences impact offspring health via germline, and epigenetic inheritance provides a potential mechanism. However, global reprogramming during embryogenesis gametogenesis represents the largest hurdle to conceptualize it. Yet, detailed characterization of how sperm alterations carrying "environmental memory" can evade embryonic remains elusive. Here, mice exposed long-term restraint stress were employed study mechanisms underlying inter- transgenerational effects paternal...
Abstract Spermatozoa acquire their fertilizing ability and forward motility during epididymal transit, suggesting the importance of epididymis. Although cell atlas epididymis was reported recently, heterogeneity cells gene expression profile in tube are still largely unknown. Considering single-cell RNA sequencing results, we thoroughly studied composition, spatio-temporal differences differentially expressed genes (DEGs) segments mitochondria throughout with sufficient numbers. In total,...
Antimony (Sb), is thought to induce testicular toxicity, although this remains controversial. This study investigated the effects of Sb exposure during spermatogenesis in Drosophila testis and underlying transcriptional regulatory mechanism at single-cell resolution. Firstly, we found that flies exposed for 10 days led dose-dependent reproductive toxicity spermatogenesis. Protein expression RNA levels were measured by immunofluorescence quantitative real-time PCR (qRT-PCR). Single-cell...
An efficient palladium-catalyzed 2-fluoroallylation of P(O)H compounds with gem-difluorocyclopropanes is presented. The reaction provides a variety 2-fluoroallylic phosphorus in good yields high Z selectivity through the sequential C-C bond activation, C-F cleavage, and C-P coupling process. Various H-phosphonates, H-phosphinates, secondary phosphine oxides are all tolerated. In addition, gram-scale synthesis late-stage modification complex bioactive molecules show practical utilities transformation.