A5079522545- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- Selenium in Biological Systems
- RNA Research and Splicing
- Nerve injury and regeneration
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Developmental Biology and Gene Regulation
- Circular RNAs in diseases
- Renal and related cancers
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Signaling Pathways in Disease
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
- Neuroscience and Neuropharmacology Research
- Hepatocellular Carcinoma Treatment and Prognosis
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Genetic Associations and Epidemiology
- Invertebrate Immune Response Mechanisms
- Cancer-related molecular mechanisms research
Nantong University
2016-2025
Chinese University of Hong Kong
2019-2025
Guangzhou Medical University
2025
Prince of Wales Hospital
2025
Wuhan Ship Development & Design Institute
2020-2024
Huazhong University of Science and Technology
2020-2024
Tongji Hospital
2020-2024
Umeå University
2019-2023
University of Chinese Academy of Sciences
2019-2023
Beijing Institute of Genomics
2019-2023
Nerve injury is a common and difficult clinical problem worldwide with high disability rate. Different from the central nervous system, peripheral system able to regenerate after injury. Wallerian degeneration in distal nerve stump contributes construction of permissible microenvironment for regeneration. To gain new molecular insights into degeneration, this study aimed identify differentially expressed genes elucidate significantly involved pathways cellular functions following Microarray...
Significance The nucleus accumbens (NAc) regulates cue-induced motivated behaviors, a process that requires fine-tuned functional output of medium spiny principal neurons (MSNs). Our present study reveals that, although contributing <1% the NAc shell (NAcSh) neuronal population, fast-spiking interneurons (FSIs) form an inhibitory feedforward circuit to dictate MSNs. Cocaine self-administration, rodent model mimicking human cocaine seeking and taking, persistently potentiates driving force...
Significance The major spliceosome is required for mRNA processing and believed to be essential cell survival. Here we report that mutations affecting the are associated with nonobstructive azoospermia (NOA), a common but poorly understood cause of male infertility in humans. Specifically, dysregulation impairs differentiation spermatogonia, abolishing maturation germ cells into sperm. requirement during spermatogonial highly conserved among species. Our study has uncovered genetic causes...
Testicular aging manifests as impaired spermatogenesis and morphological alterations in Drosophila. Nonetheless, the comprehensive molecular regulatory framework remains largely undisclosed. This investigation illustrates impact of copper overload on testicular underscores interplay between lncRNA. Copper triggers Cuproptosis through mitochondrial TCA cycle, facilitating intracellular interactions with Ferroptosis, thereby governing aging. Dysfunction lncRNA:CR43306 also contributes to...
Mammalian epididymal epithelial cells are crucial for sperm maturation. Historically, vacuole-like ultrastructures in were observed via transmission electron microscopy but undefined. Here, we utilize volume (vEM) to generate 3D reconstructions of and identify these vacuoles as intercellular organelle reservoirs (IORs) the lateral space (LIS), which contains protein aggregates, autophagosomes, lysosome-related organelles mitochondrial residues. Immunolabelling markers such P62, LC3, LAMP1...
Wallerian degeneration occurs immediately following injury to mammal peripheral nerves. To better understand the molecular events occurring during degeneration, a rat model of sciatic nerve transection was used assess differentially expressed genes at 0.5, 1, 6, 12, 24 hours, 4 days, 2, 3, and weeks post (PNI). Hierarchical clustering, Euclidean distance matrix, principal component analysis (PCA) collectively suggested three distinct phases within post-injury period weeks. Gene ontology (GO)...
The homeostasis of self-renewal and differentiation in stem cells is controlled by intrinsic signals their niche. We conducted a large-scale RNA interference (RNAi) screen Drosophila testes identified 221 genes required for germline cell (GSC) maintenance or differentiation. Knockdown these transit-amplifying spermatogonia cyst further revealed various phenotypes. Complex analysis uncovered that many the are involved key steps protein synthesis degradation. A group mRNA splicing translation...
This study aims the potential gene involved in metastasis of prostate cancer (Pca).PubMed GEO datasets (GSE6605 and GSE6606) were downloaded. We used multiple bioinformatics methods to screen differentially expressed genes Pca. Gene network was built by STRING visualized Cytoscape. All hub analyzed cBioPortal. Inhibition CDK2 including siRNA, inhibitor cas9-induced knockout followed an invasion assay. Downstream western blot.Sequencing data with expression alterations. The top validated our...
Antimony (Sb), is thought to induce testicular toxicity, although this remains controversial. This study investigated the effects of Sb exposure during spermatogenesis in Drosophila testis and underlying transcriptional regulatory mechanism at single-cell resolution. Firstly, we found that flies exposed for 10 days led dose-dependent reproductive toxicity spermatogenesis. Protein expression RNA levels were measured by immunofluorescence quantitative real-time PCR (qRT-PCR). Single-cell...
Abstract Epithelial ovarian carcinoma (EOC) is the most lethal gynecologic malignancy. However, molecular mechanisms remain unclear. In this study, we found that miR-146b was downregulated in EOC and its expression level negatively correlated with pathological staging. Follow-up functional experiments illustrated overexpression of significantly inhibited cell migration invasion, increased proliferation, but it also improved response to chemotherapeutic agents. Mechanistically, demonstrated...
During spermatogenesis, mitochondria extend along the whole length of spermatid tail and offer a structural platform for microtubule reorganization synchronized individualization, that eventually helps to generate mature sperm in Drosophila. However, regulatory mechanism during elongation remains largely unknown. Herein, we demonstrated NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42) was essential male fertility Moreover, ND-42 depletion led mitochondrial disorders Drosophila testes....
Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis tumor growth. We have previously identified MTMR7 proteomic profile mouse spermatogonial stem cell (SSC) maturation differentiation, implying that associated with neonatal testicular development. In this study, to further explore distribution function testis, we studied effect Mtmr7 knockdown on development by SSC culture methods. Our results revealed...
In males, aging is accompanied by decline in serum testosterone levels due to impairment of testicular Leydig cells. The polycomb protein BMI1 has recently been identified as an anti-aging factor. our previous study, null mice showed decreased and cell population, excessive oxidative stress p16/p19 signaling activation. However, a cause-and-effect relationship between phenotypes pathways was not investigated. Here, we used the rescue approach study role or BMI1-mediated steroidogenesis. Our...
The nucleus accumbens shell (NAcSh) regulates emotional and motivational responses, a function mediated, in part, by integrating prioritizing extensive glutamatergic projections from limbic paralimbic brain regions. Each of these inputs is thought to encode unique aspects arousal. do not operate alone, but rather are often activated simultaneously during motivated behaviors, which they can interact coordinate shaping behavioral output. To understand the anatomic physiological bases...
Testosterone biosynthesis progressively decreases in aging males primarily as a result of functional changes to Leydig cells. Despite this, the mechanisms underlying steroidogenesis remain largely unclear. Using gene knock-out approaches, we and others have recently identified Bmi1 an anti-aging gene. Herein, investigate role BMI1 using mouse MLTC-1 primary We show that can positively regulate testosterone production. Mechanistically, addition its known antioxidant activity, also report p38...
The self-renewal of spermatogonial stem cells (SSCs) requires a special microenvironment and is strictly controlled.Previously, we identified BMI1 as key regulator spermatogenesis in knock-out mouse model.However, the mechanisms by which regulates SSC maintenance remain largely unknown.Herein, show that essential for maintenance.BMI1 directs transcriptional repression target genes increasing H2AK119ub reducing H3K4me3 SSCs.Furthermore, inhibition resulted activation Wnt10b thereby promoted...
Titanium dioxide nanoparticles (TiO
The menaquinone:cytochrome creductase, or bc complex, of Bacillus subtilisbelongs to a third class bc-type distinct from the bc1 and b6 f classes. Using mutagenesis approach, we demonstrate that cytochrome b (QcrB) c (QcrC) subunits complex give rise bands at 22 29 kDa, respectively, after denaturing electrophoresis; both are required for proper assembly and/or stability; retain one heme molecule under conditions. This unusual property b-type was investigated further. We present evidence...
Alzheimer's disease (AD) is the most common neurodegenerative characterized by a progressive impairment of cognitive functions including spatial learning and memory. Excess copper exposure accelerates development AD; however, poten
Abstract The incidence of fertile women with missed abortion dramatically increased in recent years, while very few serum indices have been identified for the diagnosis abortion. aim this study was to identify related factors through a retrospective indices. A total 795 cases and 694 normal pregnancy between March 2014 2017 were included present study. based on clinical history, examination, transvaginal ultrasound findings. final assessment structures (i.e., gestational sac without fetal...