A5049792044- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- FOXO transcription factor regulation
- RNA modifications and cancer
- Renal and related cancers
- Spine and Intervertebral Disc Pathology
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Hormonal and reproductive studies
- Polyamine Metabolism and Applications
- Selenium in Biological Systems
- Prenatal Screening and Diagnostics
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Parkinson's Disease Mechanisms and Treatments
- Musculoskeletal pain and rehabilitation
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Fetal and Pediatric Neurological Disorders
Nanjing Medical University
2016-2025
Suzhou Municipal Hospital
2017-2025
Capital Medical University
2013-2023
State Key Laboratory of Reproductive Medicine
2020-2023
Fudan University
2019-2023
Soochow University
2021-2023
Henan University of Technology
2019-2023
Anhui Medical University
2023
Huashan Hospital
2019-2022
Nantong University
2021
The formation of (bi)carbonates is a pressing issue for CO2 electroreduction in neutral or alkaline solutions. It adversely causes low single-pass conversion efficiency as result (bi)carbonate crossover, well limited device lifetimes precipitation at the cathode. One emerging solution to circumvent this challenge conducting reaction acids. To end, we here demonstrate an acid-fed membrane electrode assembly (MEA) CO. A diluted electrolyte with H+ Cs+ ratio 1:1 and relatively current density...
Objective:The purpose of this study was to investigate the effects and pharmacological mechanisms icariin, which is main component in traditional Chinese herb Epimedium, on β-amyloid (Aβ) production an amyloid precursor protein (APP) transgenic (Tg) mouse model Alzheimer's disease (AD).Methods: APPV717I Tg mice were randomly divided into a group icariin-treated (30 100 μmol/kg per day) groups.Learning-memory abilities determined by Morris water maze object recognition tests.Aβ contents...
Artificial synapses that integrate functions of sensing, memory and computing are highly desired for developing brain-inspired neuromorphic hardware. In this work, an optoelectronic synapse based on the ZnO nanowire (NW) transistor is achieved, which can be used to emulate both short-term long-term synaptic plasticity. Synaptic potentiation present when device stimulated by light pulses, arising from light-induced O2 desorption persistent photoconductivity behavior NW. On other hand,...
Testicular aging manifests as impaired spermatogenesis and morphological alterations in Drosophila. Nonetheless, the comprehensive molecular regulatory framework remains largely undisclosed. This investigation illustrates impact of copper overload on testicular underscores interplay between lncRNA. Copper triggers Cuproptosis through mitochondrial TCA cycle, facilitating intracellular interactions with Ferroptosis, thereby governing aging. Dysfunction lncRNA:CR43306 also contributes to...
Abstract Background Osteosarcoma (OS) is the most common malignant tumor of bone, and clinical efficacy current treatments associated survival rates need to be further improved by employing novel therapeutic strategies. Although various studies have shown that BMI1 protein universally upregulated in OS cells tissues, its specific role underlying mechanism not yet been fully explored. Methods Expression was detected western blot. The effect on proliferation migration (143B U-2OS cell lines)...
BackgroundSequencing cell-free DNA in maternal plasma is an effective noninvasive prenatal testing technique that has been used fetal aneuploidy screening worldwide. However, its clinical application limited by the low fraction (<4%) of many singleton pregnancies, which usually results screen failures or no calls. In addition, dizygotic twin contributions into circulation can vary 2-fold, complicating quantitative diagnosis aneuploidy.ObjectiveWe performed semiconductor sequencing shorter...
During spermatogenesis, mitochondria extend along the whole length of spermatid tail and offer a structural platform for microtubule reorganization synchronized individualization, that eventually helps to generate mature sperm in Drosophila. However, regulatory mechanism during elongation remains largely unknown. Herein, we demonstrated NADH dehydrogenase (ubiquinone) 42 kDa subunit (ND-42) was essential male fertility Moreover, ND-42 depletion led mitochondrial disorders Drosophila testes....
Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis tumor growth. We have previously identified MTMR7 proteomic profile mouse spermatogonial stem cell (SSC) maturation differentiation, implying that associated with neonatal testicular development. In this study, to further explore distribution function testis, we studied effect Mtmr7 knockdown on development by SSC culture methods. Our results revealed...
In males, aging is accompanied by decline in serum testosterone levels due to impairment of testicular Leydig cells. The polycomb protein BMI1 has recently been identified as an anti-aging factor. our previous study, null mice showed decreased and cell population, excessive oxidative stress p16/p19 signaling activation. However, a cause-and-effect relationship between phenotypes pathways was not investigated. Here, we used the rescue approach study role or BMI1-mediated steroidogenesis. Our...
Testosterone biosynthesis progressively decreases in aging males primarily as a result of functional changes to Leydig cells. Despite this, the mechanisms underlying steroidogenesis remain largely unclear. Using gene knock-out approaches, we and others have recently identified Bmi1 an anti-aging gene. Herein, investigate role BMI1 using mouse MLTC-1 primary We show that can positively regulate testosterone production. Mechanistically, addition its known antioxidant activity, also report p38...
The self-renewal of spermatogonial stem cells (SSCs) requires a special microenvironment and is strictly controlled.Previously, we identified BMI1 as key regulator spermatogenesis in knock-out mouse model.However, the mechanisms by which regulates SSC maintenance remain largely unknown.Herein, show that essential for maintenance.BMI1 directs transcriptional repression target genes increasing H2AK119ub reducing H3K4me3 SSCs.Furthermore, inhibition resulted activation Wnt10b thereby promoted...
Abstract As a common malignant tumor among women, ovarian cancer poses serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in tissues using patient sample and tissue microarrays. In addition, shown promote cell proliferation metastasis both vitro vivo. Mechanistically, stabilizes protein tyrosine kinase 2 (PTK2) activate the β-catenin pathway via repressing MG-53-mediated ubiquitinated degradation of PTK2, thereby facilitating...
Testicular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With high-throughput quantitative proteomics technology, a comparative proteomic profile mouse embryonic gonads were analyzed at three time points (11.5, 12.5, and 13.5 days post coitum), corresponding to critical stages testicular gonadal development. 4070 proteins identified, 338 differentially expressed, which Sertoli specific genes significant...
The cellular nucleic acid-binding protein (CNBP), also known as zinc finger 9, is a highly conserved that strikingly among vertebrates. Data collected from lower vertebrates showed CNBP expressed at high levels and distributed in the testes during spermatogenesis. However, location function of mammalian are not well known. Here, by neonatal mouse testis culture spermatogonial stem cells (SSC) methods, we studied effect knockdown on testicular development. Our results revealed was mainly...
Background Retinoic Acid Induced Protein 14 ( Rai14 ) is an evolutionarily conserved gene that highly expressed in the testis. Previous experiments have reported small interfering RNA (siRNA)-mediated knockdown (KD) of rat testis disrupted spermatid polarity and transport. Of note, a knockout (KO) model considered “gold standard” for vivo assessment crucial functions. Herein, we used CRISPR/Cas9-based editing to investigate role mouse Methods Sperm concentration motility were assayed using...
Cognitive dysfunctions have been reported in multiple system atrophy (MSA). However the underlying mechanisms remain to be elucidated. This study aimed explore possible cerebral metabolism associated with domain-specific cognitive performances MSA.A total of 84 patients were diagnosed as probable or MSA, comprised 27 MSA predominant parkinsonism (MSA-P) and 57 cerebellar ataxia (MSA-C). The comprehensive neuropsychological tests 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography...
SPRY (repeats in splA and RyR) domain-containing SOCS (suppressor of cytokine signaling) box protein 1 (SPSB1) is an E3 ligase adaptor that has been implicated various cellular processes physiological pathways. However, its role spermatogenesis remains poorly understood. The objective this study was to investigate the impact SPSB1 deficiency on male fertility mice. We generated Spsb1 knockout (Spsb1-KO) mice explore effects sperm quality. To assess parameters, we utilized computer-assisted...
As a crucial member of the Suppressor Cytokine Signaling (SOCS) family, SOCS7 regulates various physiological processes, including insulin resistance, inflammation, and tumor suppression. However, its role in male germ cells remains poorly understood. This study aims to investigate function spermatogenesis uncover potential regulatory mechanisms. We conducted bioinformatics analyses examine expression profile Socs7 testes, generated Socs7-knockout (KO) mice using CRISPR/Cas9 genome editing,...
Spermatogenesis, the process by which male germ cells develop into mature spermatozoa, is a complex and highly regulated phenomenon crucial for fertility. Various molecular pathways, including ubiquitination, play critical roles in this process. Ubiquitination regulates multiple stages of spermatogenesis controlling cell remodeling protein metabolism. SplA/ryanodine receptor domain SOCS box containing 3 (SPSB3), protein, interacts with ElonginC/B recruits Cullin5 to form ECS E3 ligase...
The precise cause of false-positive results in non-invasive prenatal testing (NIPT) associated with suspected placental chimerism - where the placenta exhibits chromosomal abnormalities absent fetus, is not well understood. It also unclear whether serum minerals such as zinc (Zn) and calcium (Ca), which are essential for maternal fetal health, influence these false positives. In this retrospective analysis, 16,270 pregnant women were evaluated mineral concentrations at 14 weeks gestation,...
Testicular aging has profound effects on spermatogenesis, sperm function, and the spermatogenic microenvironment, contributing to reduced male fertility. However, precise molecular mechanisms by which mitochondria influence spermiogenesis during still remain largely unclear. Vha68-3 KO flies were generated using CRISPR/Cas9 technique. phenotypes functions mainly observed through immunofluorescence staining transmission electron microscopy. Multi-omics study was conducted single-cell RNA...
While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, mechanisms SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in proteome profile for mouse maturation differentiation, revealing SBNO1 associated with neonatal testicular development. To explore further location function testes, we performed Sbno1 gene knockdown...
Ubiquitination is the most common post-translational modification and essential for various cellular regulatory processes. RNF187, which known as RING domain AP1 coactivator-1, a member of finger family. RNF187 can promote proliferation migration tumor cells. However, whether it has similar role in regulating spermatogonia not clear. This study explored molecular mechanism mouse cell line (GC-1). We found that knockdown reduced GC-1 cells promoted their apoptosis. overexpression...
Primordial germ cells (PGCs) are embryonic founders of that ultimately differentiate into oocytes and spermatogonia. Embryonic proliferation PGCs starting from E11.5 ensures the presence in adulthood, especially female mammals whose total number declines after this initial period. To better understand mechanisms underlying PGC mice, we constructed a proteome profile mouse gonads at E11.5. Subsequent KEGG pathway analysis 3,662 proteins profiled showed significant enrichment pathways involved...