A5056265391- Cancer, Stress, Anesthesia, and Immune Response
- Axon Guidance and Neuronal Signaling
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Brain Metastases and Treatment
- Prostate Cancer Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Neuroendocrine Tumor Research Advances
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- Neurobiology and Insect Physiology Research
- Single-cell and spatial transcriptomics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microtubule and mitosis dynamics
- Vagus Nerve Stimulation Research
- Developmental Biology and Gene Regulation
- Prostate Cancer Treatment and Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Oregon Health & Science University
2020-2025
OHSU Knight Cancer Institute
2021
University of Illinois Chicago
2018
Abstract Identifying precise molecular subtypes attributable to specific stages of localized prostate cancer has proven difficult due high levels heterogeneity. Bulk assays represent a population-average, which mask the heterogeneity that exists at single-cell level. In this work, we sequence accessible chromatin regions 14,424 single-cells from 18 flash-frozen tumours. We observe shared features among low-grade cells are lost in high-grade Despite loss, tumours exhibit an enrichment for...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers, with a 5-year survival rate 12.8%. This aggressive malignancy composed heterogenous tumor microenvironment (TME), including cancer-associated fibroblasts (CAFs) and sympathetic neurons. The abundance CAFs neurons correlates progression often associated poor prognosis PDAC patients. However, precise role CAF-nerve interactions in cancer metastasis remains unclear. To explore our hypothesis that these...
Hox genes are involved in the patterning of animal body parts at multiple levels regulatory hierarchies. Early expression different domains along embryonic anterior-posterior (A/P) axis insects, vertebrates, and other animals establishes segmental or regional identity. However, gene function is also required later development for morphogenesis limbs organs. In Drosophila, spatiotemporal modulation Sex combs reduced (Scr) within first thoracic (T1) leg underlies generation segment-...
Cancer is a highly heterogenous disease that requires precise detection tools and active surveillance methods. Liquid biopsy assays provide an agnostic way to follow the complex trajectory of cancer, providing better patient stratification for optimized treatment. Here, we present development low-volume liquid assay called cyc-DEP (cyclic immunofluorescent imaging on dielectrophoretic chip) profile biomarkers collected microfluidic chip platform. To enable on-chip cyclic imaging, fluorophore...
SUMMARY Neurosignaling is increasingly recognized as a critical factor in cancer progression, where neuronal innervation of primary tumors contributes to the disease’s advancement. This study focuses on segmenting individual axons within prostate tumor microenvironment, which have been challenging detect and analyze due their irregular morphologies. We present novel deep learning-based approach for automated segmentation axons, AxonFinder, leveraging U-Net model with ResNet-101 encoder,...
We present a follow-on technique for the cyclic-immunofluorescence profiling of suspension particles isolated using dielectrophoresis. The original lab-on-chip ("cyc-DEP" [cyclic immunofluorescent imaging on dielectrophoretic chip]) was designed multiplex surveillance circulating biomarkers. Nanoparticles were collected from low-volume liquid biopsies microfluidic chip technology. Subsequent rounds cyclic labeling and quenching imaged quantified with custom algorithm to detect multiple...