A5089314678- Polyamine Metabolism and Applications
- Marine Biology and Ecology Research
- Marine Ecology and Invasive Species
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Marine Bivalve and Aquaculture Studies
- RNA modifications and cancer
- RNA Research and Splicing
- Parasite Biology and Host Interactions
- Identification and Quantification in Food
- Marine Biology and Environmental Chemistry
- Genomics and Phylogenetic Studies
- Aquatic Invertebrate Ecology and Behavior
- Beetle Biology and Toxicology Studies
- Cannabis and Cannabinoid Research
- Mollusks and Parasites Studies
- Genetic diversity and population structure
- Amphibian and Reptile Biology
- Coral and Marine Ecosystems Studies
- Coleoptera Taxonomy and Distribution
- Marine and environmental studies
- Machine Learning in Bioinformatics
- Mitochondrial Function and Pathology
- Species Distribution and Climate Change
- Cassava research and cyanide
Roma Tre University
2015-2024
Sapienza University of Rome
1995-2024
University of Palermo
2024
Marconi University
2000-2018
Universidad de Cádiz
2014
National Agency for New Technologies, Energy and Sustainable Economic Development
2009
University of Rome Tor Vergata
1988-1995
University of Parma
1993-1995
Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare
1993
Istituto di Biologia e Biotecnologia Agraria
1993
We have compiled a complete list of new marine molluscan taxa introduced by Tommaso Allery Di Maria, Marquis Monterosato (1841-1927). The dates publication every single work been checked against available evidence, and an updated bibliography is also presented. Finally, the type material all expected to be in collection (presently preserved Museo Civico di Zoologia Rome) has searched main collection, retrieved specimens catalogued. A large majority found, representative each taxon...
'Cryptic' species are an emerging biological problem that is broadly discussed in the present study. Recently, a cryptic definition was suggested for those which manifest low morphological, but considerable genetic, disparity. As case study we unique material from charismatic group of nudibranch molluscs genus Trinchesia European waters to reveal three new and demonstrate they show dual nature: on one hand, can be considered 'cryptic' complex due their overall similarity, other stable...
The diversity of Mediterranean nudibranchs has yet to be thoroughly studied: new species are constantly described, and molecular approaches have revealed some cryptic species. A facelinid been discovered based on specimens collected from the Tyrrhenian Sea (Mediterranean Sea). Integrative results analyses anatomical investigations support description Dondice trainitoi sp. nov. characteristic chromatic body pattern black epithelium covering masticatory jaws allow an unambiguous identification...
Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react fibrillarin, a 34- 36-kilodalton protein that has been detected in all eukaryotes tested from humans yeasts. We isolated 1.6-kilobase cDNA encoding fibrillarin Xenopus laevis library. The contains 79-residue-long Gly-Arg-rich domain its N-terminal region and putative RNA-binding consensus sequence central portion. This is the first report of cloning deduced...
The bivalve family Ostreidae has a worldwide distribution and includes species of high economic importance. Phylogenetics systematic oysters based on morphology have proved difficult because their phenotypic plasticity. In this study we explore the phylogenetic information DNA sequence secondary structure nuclear, fast-evolving, ITS2 rRNA mitochondrial 16S genes from implemented multi-locus framework four loci for oyster phylogenetics systematics. Sequence-structure models aid alignment...
Molecular approaches have contributed to a drastic reconsideration of organisms' systematics and evolution especially in some groups bivalves where high levels phenotypic plasticity hampered morphology-based assessments. However, these insights rarely been integrated into taxonomy classification due the challenge taxon description based on DNA data alone. In this study we used, for first time, an approach ITS2 rRNA sequence-structure diagnosis new oyster taxa identified multi-locus...
Abstract Exogenously supplied auxin (1-naphthaleneacetic acid) inhibited light-induced activity increase of polyamine oxidase (PAO), a hydrogen peroxide-producing enzyme, in the outer tissues maize (Zea mays) mesocotyl. The same phenomenon operates at PAO protein and mRNA accumulation levels. wall-bound to extractable ratio was unaffected by treatment, either dark or after light exposure. Ethylene treatment did not affect activity, thus excluding an effect via increased ethylene...
The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by combined approach nucleotide and peptide analysis. A cDNA 1737 bp, isolated from maize seedlings reverse transcription-polymerase chain reaction rapid amplification ends strategies, cloned its determined. This contains information for polypeptide 500 acids. Its amino-terminal shows the typical features secretion signal peptides. primary structure mature protein independently confirmed extensive...
Nucleolin is a key nucleolar protein in higher eukaryotic cells and involved directly ribosome biogenesis. Using an antiserum raised against hamster nucleolin, the homologous was detected nucleoli of Xenopus laevis hepatocytes as well amplified oocytes. A cDNA encoding nucleolin has been isolated sequenced. The deduced sequence reveals similar domains mammals, but they have undergone separate evolutions. In particular, each four RNA-binding evolved differently--the carboxy-proximal domain...
During Xenopus development, the synthesis of ribosomal proteins is regulated at translational level. To identify region protein mRNAs responsible for their typical behavior, we constructed a fused gene in which upstream sequences (promoter) and 5' untranslated sequence (first exon) coding S19 were joined to portion procaryotic chloramphenicol acetyltransferase (CAT) deleted its own region. This was introduced vivo by microinjection into fertilized eggs, activity monitored during...
Previously, we reported that HIV-Tat elicits spermine oxidase (SMO) activity upregulation through NMDA receptor (NMDAR) stimulation in human SH-SY5Y neuroblastoma cells, thus increasing ROS generation, which turn leads to GSH depletion, oxidative stress, and reduced cell viability. In several types, can trigger an antioxidant response the transcriptional induction of stress-responsive genes regulated by nuclear factor erythroid 2-related 2 (Nrf2). Here, demonstrate Tat induces both gene...
Polyamine oxidases are key enzymes responsible of the polyamine interconversion metabolism in animal cells. Recently, a novel enzyme belonging to this class has been characterized for its capability oxidize preferentially spermine and designated as oxidase. This is flavin adenine dinucleotide-containing enzyme, it expressed both vitro vivo systems. The primary structure mouse oxidase (mSMO) was deduced from cDNA clone (Image Clone 264769) recovered by data base search utilizing human...
Abstract Aim We discuss biogeographical hypotheses for the Mediterranean lizard species Podarcis and Teira within a phylogenetic framework based on partial mitochondrial DNA sequences. Methods derived most likely hypothesis from our data set (597 aligned positions 12S rDNA phenyl tRNA) under parsimony, distance maximum likelihood assumptions. Results The usually included in do not form strongly monophyletic clade. In contrast, monophyly of genus is rather well supported. Seven lineages are...
Spermine oxidase is a FAD-containing enzyme involved in polyamines catabolism, selectively oxidizing spermine to produce H2O2, spermidine, and 3-aminopropanal. highly expressed the mouse brain plays key role regulating levels of spermine, which protein synthesis, cell division growth. normally released by neurons at synaptic sites where it exerts neuromodulatory function, specifically interacting with different types ion channels, ionotropic glutamate receptors. In order get an insight into...
Two cDNAs encoding polyamine oxidase (PAO) isoforms (BPAO1 and BPAO2) the corresponding gene copies were isolated from barley cultivar Aura. Gene organization is not conserved between these two nonallelic coding sequences. Both precursor proteins include a cleavable N‐terminal leader of 25 amino acids. sequencing PAO purified seedlings reveals unique amino‐acid sequence to BPAO2 N‐terminus as predicted cDNA. has been purified, characterized compared maize (MPAO), best member this enzyme...
Polyamine oxidase (PAO) and spermine (SMO) are involved in the catabolism of polyamines--basic regulators cell growth proliferation. The discovery selective inhibitors PAO SMO represents an important tool studying involvement these enzymes polyamine homeostasis a starting point for development novel antineoplastic drugs. Here, comparative study on murine (mPAO) (mSMO) inhibition by analogues 1,8-diaminooctane, 1,12-diaminododecane, N-prenylagmatine (G3), guazatine...
Spermine oxidase (SMO) is a flavoenzyme involved in polyamine homeostasis animal cells. The mouse spermine gene (mSMO) codes for splice variants, including the previously reported major active isoform, herein named alfa (alpha). In present work, eight additional splicing variants were characterized. heterologous expression and biochemical characterization of three recombinant isoforms (namely mSMOmu, -gamma -delta) revealed that only protein mSMO micro displays characteristics similar to...