A5034023721- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Immunotherapy and Immune Responses
- Intensive Care Unit Cognitive Disorders
- Tracheal and airway disorders
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Caveolin-1 and cellular processes
- Asthma and respiratory diseases
- Lymphatic Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Mechanisms of cancer metastasis
- Respiratory and Cough-Related Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Advanced Proteomics Techniques and Applications
- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Cancer, Stress, Anesthesia, and Immune Response
- Colorectal and Anal Carcinomas
- Neuroblastoma Research and Treatments
- Eosinophilic Esophagitis
- Menopause: Health Impacts and Treatments
- Intramuscular injections and effects
Johns Hopkins University
2021-2024
Johns Hopkins Medicine
2022-2024
The University of Melbourne
2012-2017
University of Connecticut
2017
St Vincent Medical Center
2014
St Vincent Hospital
2014
Royal Women's Hospital
2010-2014
UConn Health
2014
St Vincent's Hospital
2012
Epithelial mesenchymal transition (EMT) and cancer stem cells (CSC) have been associated with resistance to chemotherapy. Eighty percent of ovarian patients initially respond platinum-based combination therapy but most return recurrence ultimate demise. To better understand such chemoresistance we assessed the potential role EMT in tumor collected from advanced-stage cell line OVCA 433 response cisplatin vitro. We demonstrate that cisplatin-induced epithelial morphology residual correlated...
Tumor cells in ascites are a major source of disease recurrence ovarian cancer patients. In an attempt to identify and profile the population obtained from patients, novel method was developed separate adherent (AD) non-adherent (NAD) culture. Twenty-five patients were recruited this study; 11 chemonaive (CN) 14 chemoresistant (CR). AD both CN CR exhibited mesenchymal morphology with antigen stem fibroblasts. Conversely, NAD had epithelial enhanced expression 125 (CA125), cell adhesion...
Abstract Over 80% of women diagnosed with advanced-stage ovarian cancer die as a result disease recurrence due to failure chemotherapy treatment. In this study, using two distinct cell lines (epithelial OVCA 433 and mesenchymal HEY) we demonstrate enrichment in population cells high expression CSC markers at the protein mRNA levels response cisplatin, paclitaxel combination both. We also significant enhancement sphere forming abilities drugs. The results these vitro findings are supported by...
Abstract Background: AXA-042 is a novel, systemically delivered synthetic toll-like receptor (TLR) 2/6 agonist in development for the treatment of advanced solid tumors. A first-in-human study evaluating safety, tolerability, pharmacokinetics, and pharmacodynamics as monotherapy combination with checkpoint inhibitor subjects tumors currently ongoing (ACTRN12622000993796). Cytometry by time-of-flight (CyTOF) analysis was performed to characterize immune modulation signatures patient whole...
Melanoma is an aggressive type of skin cancer, which accounts for only 4% cancer cases but causes around 75% deaths. Currently, there a limited set protein biomarkers that can distinguish melanoma subtypes and provide accurate prognosis melanoma. Thus, we have selected profiled the proteomes five different cell lines from stages progression in comparison with normal melanocytes using tandem mass spectrometry. We also proteome solid metastatic tumor. This resulted identification 4758 unique...
In view of the recent association Brn-3 transcription factors with neuroblastomas, cervical, breast, and prostate cancers we examined expression Brn-3a(l) in normal ovaries different histological grades ovarian tumors. The was also evaluated cancer cell lines tumor cells isolated from ascites advanced-stage patients.Normal ovaries, benign, borderline, 1, 2 3 tumors were analyzed by immunohistochemistry for expression. A total 46 specimens included study. Immunofluorescence used to...
Seventy percent of ovarian cancer patients die due to consecutive episodes recurrences resulting from the re-growth tumor cells resistant conventional chemotherapies.In an effort identify chemoresistance mechanisms, we compared expression genes in isolated ascites advanced-stage serous prior (chemonaive, CN) and after chemotherapy treatments (chemoresistant/recurrent, CR).A novel, recently published method was used for isolation CN CR patients.Illumina HT-12 platform assess differential...
Chemotherapy resistance is a major obstacle for the treatment of ovarian cancer patients.Combination drugs which can exert synergistic effect be promising strategy to overcome this resistance.In study, we report significantly enhanced activation Janus kinase 2 (JAK2) and its downstream target signal transducer transcription 3 (STAT3) in tumor cells isolated from ascites recurrent patients (CR) compared chemonaïve (CN).Enhanced JAK2 STAT3 coincided with vitro pathway cisplatin-surviving cell...
Introduction Patients with COVID-19 ARDS require significant amounts of sedation and analgesic medications which can lead to longer hospital/ICU length stay, delirium, has been associated increased mortality. Tracheostomy shown decrease the amount sedative, anxiolytic given patients. The goal this study was assess whether tracheostomy decreased medication usage, improved markers activity level cognitive function, clinical outcomes in patients ARDS. Study Design Methods A retrospective...
Recent advances in cancer immuno-therapeutics such as checkpoint inhibitors, chimeric antigen-receptor T cells, and tumor infiltrating cells (TIL) are now significantly impacting patients a positive manner. Although very promising, reports indicate no more than 25% of cases result complete remission. One the limitations these treatments is identity putative antigens each patient, it technically challenging to identify rapid fashion. Thus, identification followed by targeted treatment will...
Eosinophilic Plastic Bronchitis following COVID.docavailable at
The authors declare no conflict of interest. Data sharing is not applicable to this article as new data were created or analyzed in study. Please note: publisher responsible for the content functionality any supporting information supplied by authors. Any queries (other than missing content) should be directed corresponding author article.
Abstract PURPOSE: The treatment of ovarian cancer (OC) with chemotherapy leaves resistant cells which in a short time re–grow as recurrent cancer. A diverse array resistance mechanisms for tumor has been described but none proven to be viable target clinical setting. Cancer stem (CSCs) are increasingly accepted the putative mediators chemoresistance and relapse This study aimed understand molecular involved recurrence by investigating roles CSCs their associated pathways OC cell lines...
<b>Intro:</b> Patients intubated with COVID-19 ARDS have required significant amounts of sedation and analgesic medications (SAM). This can lead to longer length hospital/ICU stay, days delirium, has been associated mortality. We aimed evaluate SAM administration in patients undergoing tracheostomy resultant from ARDS. <b>Methods:</b> A retrospective registry all who had a consult for placement at the Johns Hopkins Hospital during months 03/2020 - 08/2020. Data were collected by chart review...