The human amniotic membrane (HAM) contains two cell types from different embryological origins. Human amnion epithelial cells (hAECs) are derived the embryonic ectoderm, while mesenchymal stromal (hAMSCs) mesoderm. In this study, we localized, isolated, quantified and phenotypically characterized HAM-derived analysed their in vitro differentiation potential towards mesodermal lineages. amnion-derived were isolated by flow cytometry. Immunohistochemistry quantitative real-time reverse...

Mesenchymal stem cells (MSCs) have the capacity to differentiate into several cell lineages, some of which can generate bone, cartilage, or adipose tissue. The presence MSCs in synovial membrane was recently reported. Data from comparative studies derived various mesenchymal tissues suggest that membranes a superior chondrogenesis capacity. Previous chondrogenic differentiation used total population MSCs, including with MSC markers, such as CD44, CD90, CD105, CD73. However an individual has...

Background . The interests in mesenchymal stem cells (MSCs) and their application cell therapy have resulted a better understanding of the basic biology these cells. Recently hypoxia has been indicated as crucial for complete chondrogenesis. We aimed at analyzing bone marrow MSCs (BM-MSCs) differentiation capacity under normoxic severe hypoxic culture conditions. Methods were characterized by flow cytometry differentiated towards adipocytes, osteoblasts, chondrocytes or differentiations...

Objective. To quantify cells expressing mesenchymal stem cell (MSC) markers in synovial membranes from human osteoarthritic (OA) and healthy joints. Methods. Synovial OA joints were digested with collagenase the isolated cultured. membrane-derived phenotypically characterized for differentiation experiments using flow cytometry to detect expression of (CD29, CD44, CD73, CD90, CD105, CD117, CD166, STRO-1) hematopoietic (CD34 CD45). Chondrogenesis was assessed by staining proteoglycans...

Introduction: The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine cell therapy. Aim: To compare two previously published protocols the isolation of amnion mesenchymal stromal cells (hAMSCs), including their phenotypic characterization in vitro potential differentiation toward osteogenic, adipogenic, chondrogenic mesodermal lineages. Materials Methods: Human placentas were obtained from selected caesarean-sectioned births....

As myofascial release therapy is currently under development, the objective of this study was to compare effectiveness with manual for treating occupational mechanical neck pain.A randomized, single-blind parallel group developed. The sample (n = 59) divided into GI, treated therapy, and GII, therapy. Variables studied were intensity pain, cervical disability, quality life, craniovertebral angle, ranges motion.At five sessions, clinical significance observed in both groups all variables...

The purposes of this project were to quantify the cells expressing mesenchymal stem cell (MSC) marker CD271 in synovial membranes from human osteoarthritic (OA) and healthy joints, determine if those involved spontaneous cartilage repair beneficial for articular defects.The coexpression CD44/CD271, CD90/CD271, CD105/CD271 antigens was determined by immunofluorescence OA during repair. Isolated MSCs bone marrow four patients (mean age: 64 years) magnetically separated into MSC CD271+ CD271-...

Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment osteochondral diseases.The aim was to isolate and characterize from bone marrow aspirates (oBMSCs) assess their usefulness repair using β-tricalcium phosphate (bTCP) type I collagen (Col I) scaffolds.Cells isolated were characterized morphologically, phenotypically, functionally. oBMSCs cultured osteogenic medium on...

Osteoarthritis (OA) is a prevalent joint disorder that lacks effective therapies to halt cartilage degeneration. Mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) are being investigated as promising chondroprotective agents. Compared primary MSCs, induced pluripotent stem (iPSC)-derived MSCs (MLCs) offer superior scalability and enhanced paracrine activity. The aim of this study was explore the feasibility using autologous MLC-derived sEVs potential therapeutic...

Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) is a naturally occurring biopolymer belonging to the polyhydroxyalkanoate (PHA) family. Due its excellent properties (biocompatible, biodegradable, and non-toxic), this presented as very suitable option for use in regenerative therapy drug delivery system (DDS). The protein encapsulated study transforming growth factor β3 (TGF-β3), which plays key role chondrogenic differentiation of mesenchymal stem cells (MSCs). main objective work evaluate...

Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like using a new in vitro model that consists of the random formation spheroids medium supplemented with fetal bovine serum on nonadherent surface. The was changed after 2 days to one specific for induction chondrocyte differentiation. We assessed this reverse transcriptase-polymerase chain reaction, flow cytometry, immunohistochemistry, and secretome analyses. purpose study determine which proteins...

Localized trauma-derived breakdown of the hyaline articular cartilage may progress toward osteoarthritis, a degenerative condition characterized by total loss and joint function. Tissue engineering technologies encompass several promising approaches with high therapeutic potential for treatment these focal defects. However, most research in tissue is focused on materials structural cues, while little attention directed to appropriate source cells endowing materials. In this study, using...

Articular cartilage has only a limited ability to regenerate. The transplantation of autologous chondrocytes is currently used treat focal defects in human articular cartilage, although use organs, tissues, or cells from different species being investigated as an alternative treatment. object this study was xeno‐transplantation cultured pig for the repair rabbit chondral defects, and analyze significance tissue rejection animal model. Partial including removal part subchondral bone, were...

After a few passages of in vitro culture, primary human articular chondrocytes undergo senescence and loss their phenotype. Most the available chondrocyte cell lines have been obtained from cartilage tissues different diarthrodial joints, utility for osteoarthritis (OA) research is reduced. Thus, goal this was development immortalized proceeded patients with without OA.Using telomerase reverse transcriptase (hTERT) SV40 large T antigen (SV40LT), we transduced OA chondrocytes. Proliferative...

Objectives: To compare the proliferative and chondrogenic potential of fresh frozen chondrocytes isolated from superficial deep articular cartilage biopsies. Materials Methodology: The study included 12 samples healthy human knee cartilage. Cell proliferation was tested at 3, 6 9 days. Studies mRNA quantification, protein expression immunofluorescence for markers were performed. Results: Stimulation both with fetal bovine serum produced an increase in capacity compared to non-stimulated...

Reprogramming somatic cells toward pluripotency became possible over a decade ago. Since then, induced pluripotent stem (iPSCs) have served as versatile and powerful tool not only for basic research but also with the long-term goal of using them in human cell transplantation after differentiation. Nonetheless, downstream applications are frequently blurred by difficulties that researchers to face when working iPSCs, such trouble clonal selection, vitro culture cryopreservation, adaptation...

The aim of this study was to evaluate proliferation and chondrogenic differentiation human bone-marrow mesenchymal stromal cells (hBMSCs) cultured on collagen biomaterials.hBMSCs were seeded five different (Col) sponges: C1C2 (types I II Col), C1C2HS Col plus heparan sulphate (HS)), C1C2CHS chondroitin (CHS)), C1-OLH3 (type low molecular weight heparin) C1CHS CHS). resulting constructs analyzed by histological immunohistochemical staining, biology electron microscopy. released into culture...