Head and neck squamous cell carcinoma (HNSCC) patients with human papillomavirus (HPV) infection have better prognosis than those without HPV infection. Although p16(INK4a) expression is used as a surrogate marker for infection, there controversy to whether reliably indicates Here, evaluate the accuracy of determining prognostic value HNSCC survival, especially oropharyngeal (OPSCC) 150 fresh-frozen samples were analyzed DNA, E6/E7 mRNA by polymerase chain reaction immunohistochemistry. was...

Abstract Background The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types head neck squamous cell carcinoma (HNSCC). Methods HPV DNA, E6/E7 mRNA expression, viral load, 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)‐based methods. Results genome detected 54 samples (29.3%), particularly tonsillar carcinomas (69.6%). Compared nonoropharyngeal HNSCC, oropharyngeal harbored a...

To investigate the prevalence, genotypes, and prognostic values of Epstein-Barr virus (EBV) human papillomavirus (HPV) infections in Japanese patients with different types head neck cancer (HNC).HPV EBV DNA, genotypes LMP-1 variants, HPV mRNA expression were detected by PCR from fresh-frozen HNC samples. determined direct sequencing, encoded RNA (EBER) was examined situ hybridization.Of 209 patients, 63 (30.1%) had infection, HPV-16 most common subtype (86.9%). E6/E7 found 23 60 (38.3%)...

To clarify the synergistic influence of human papillomavirus (HPV) status and squamous cell carcinoma antigen (SCCA) mRNA expression on head neck (HNSCC) prognosis, HPV DNA presence SCCA1 SCCA2 were determined by PCR quantitative real-time RT-PCR, respectively, in 121 patients with primary HNSCC who receiving curative treatment. was detected 28.1% (34/121) cases, only high-risk types (HPV-16, HPV-33, HPV-35 HPV-58) observed. Positive showed a significantly better prognosis than negative (P =...

We aimed to clarify the possible role of human papillomavirus (HPV) infection in malignant transformation sinonasal inverted papilloma (IP).Subjects comprised 32 patients with chronic rhinosinusitis (CRS), 17 IP, 5 IP and squamous cell carcinoma (IP + SCC), 16 primary SCC. HPV presence, viral loads, physical status were investigated using polymerase chain reaction. Retinoblastoma (pRb), p53, p16(INK4a) gene products by immunohistochemistry.HPV DNA was detected 6.3 % cases CRS, 29.4 40 SCC,...

To investigate oral candidiasis in patients with head and neck cancer before, during, after radiation therapy, to explore its association clinical oropharyngeal symptoms.A cohort study.University hospital.Subjects who received therapy (RT) for the treatment of were divided into two groups: an cavity irradiated group (OIRR group, n = 29) nonirradiated (ONIRR 17). A control consisted 18 healthy subjects. Patients examined signs immediately after, one month RT. Mouth throat soreness (MTS),...

The aim of this study was to evaluate the 8th edition American Joint Committee on Cancer Staging Manual: Head and Neck Section oropharyngeal squamous cell cancer (OPSCC) clarify relationship between p16 overexpression presence human papillomavirus (HPV) DNA using fresh frozen samples. Samples from 100 OPSCC patients were analyzed polymerase chain reaction (PCR) immunohistochemistry. Five-year overall survival (OS) 73.0%, 93.9%, 62.2% in all, p16-positive (n = 34), p16-negative 66) cases,...

<title>Abstract</title> The cytochrome b5 reductase (CYB5R) family of enzymes, consisting five members, has been reported to profoundly affect various physiological and pathological processes. However, it remains unclear how CYB5Rs function have prognostic value in cervical squamous cell carcinoma endocervical adenocarcinoma (CESC). In this study. Gene expression data clinical details 304 CESC patients were acquired from Cancer Genome Atlas (TCGA) database. Data processing visualization...

This study investigated prospectively the role of human papillomavirus (HPV) in paranasal inverted papilloma (IP). HPV presence and viral load physical status HPV-16 were examined by polymerase chain reaction-based methods using fresh frozen samples obtained from 13 patients with IP (IP group), 11 squamous cell carcinoma maxillary sinus (SCC group) 39 chronic inflammatory lesions (inflammatory group). The genome was detected 46.1%, 27.3% 7.6% IP, SCC groups, respectively. group showed...

Serum squamous cell carcinoma antigen (SCCA) levels are elevated in sinonasal inverted papilloma (IP). However, the relationship between tumor volume and SCCA level, influence of skin or pulmonary diseases which level is high, have not been established.To clarify whether serum can be used as a diagnostic marker IP.Serum was measured 30 patients with IP (IP group) 57 inflammatory disease (inflammatory group).Overall, 83.3% group showed regardless they were new recurrent IP, rapidly decreased...

Several studies have investigated hypopharyngeal cancer (HC) risk in combination with xenobiotic metabolism-related genetic polymorphisms and the burden of alcohol consumption smoking European countries but not East Asian countries.This hospital-based case-control study involved 61 male patients HC 71 cancer-free controls. Information on age, body mass index, cigarette was obtained from medical records, a self-completion questionnaire, thorough interview by an otolaryngologist. Alcohol...

Transcription factors containing a CCCH structure (C3H) play important roles in plant growth and development, their stress response, but research on the C3H gene family potato has not been reported yet. In this study, we used bioinformatics to identify 50 genes named them StC3H-1 StC3H-50 according location chromosomes, analyzed physical chemical properties, chromosome location, phylogenetic relationship, structure, collinearity cis-regulatory element. The expression pattern analysis showed...

Background The clinical importance of serum squamous cell carcinoma antigen (SCCA) and SCCA subclasses has not been established for treating inverted papilloma (IP). aim this study was to clarify the its in IP, compared with maxillary inflammatory disease. Methods Serum measured 22 patients IP (IP group), 11 (carcinoma disease (inflammatory group). mRNA expression examined using quantitative real-time polymerase chain reaction. Results In group, 81.8% showed elevated SCCA, 90.3% recurrent...

Abstract Background Head and neck squamous cell carcinoma (HNSCC) is the 6th most common malignant cancer type worldwide. Radiosensitivity has been shown to be significantly increased in patients with human papillomavirus (HPV)-positive HNSCC compared HPV-negative patients. However, clinical significance of HPV its regulatory mechanisms are largely unknown. The aim our study was explore mechanism miR-27a-3p radiosensitivity HPV-positive cells. Methods E6-overexpressing E6-knockdown lines...

Background: This study investigated prospectively the role of human papillomavirus (HPV) in paranasal inverted papilloma (IP). Methods: HPV presence and viral load physical status HPV-16 were examined by polymerase chain reaction-based methods using fresh frozen samples obtained from 13 patients with IP (IP group), 11 squamous cell carcinoma maxillary sinus (SCC group) 39 chronic inflammatory lesions (inflammatory group). Results: The genome was detected 46.1%, 27.3% 7.6% IP, SCC groups,...

Purpose: Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent malignant tumor worldwide, radiotherapy effect strongly associated with human papillomavirus (HPV) infection. Therefore, aim of our study was to analyze mechanism HPV E7 its effects on radiosensitivity in HNSCC cells. Methods: The mRNA expression DiGeorge syndrome critical region gene 8 (DGCR8), has-miR-106a, Runt-related transcription factor 3 (RUNX3) examined by quantitative real-time PCR (RT-qPCR). protein...

Objective To map comprehensively the methylation status of CpG sites within HPV16 long control region (LCR) in HPV-positive cancer cells, and to explore further effects LCR on cell bioactivity E6 E7 expression. In addition, analyze oropharyngeal squamous carcinoma (OPSCC) patients. Methods Materials Methylation patterns UM-SCC47, CaSki, SiHa cells HPV16-positiive OPSCC specimens were detected by bisulfite-sequencing PCR TA cloning. For treated with 5-aza-2′-deoxycytidine knockdown, MTS...

High‑risk human papillomavirus (HPV) DNA has been reported to be present in branchial cleft cysts, but further information is required clarify the role of HPV infection cysts. The presence HPV, viral load and physical statuses samples from six patients with cysts were investigated using polymerase chain reaction (PCR), quantitative PCR, situ hybridization (ISH) probes p16INK4a immunohistochemical analysis. type HPV‑16 was identified four analyzed. Of HPV‑positive three exhibited mixed‑type...