A5021156166- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Diverse Cultural and Historical Studies
- Anthropological Studies and Insights
- Martial Arts: Techniques, Psychology, and Education
- African Studies and Ethnography
- Urban and sociocultural dynamics
- Diffusion and Search Dynamics
- Diversity and Impact of Dance
- Gene Regulatory Network Analysis
- Aging, Elder Care, and Social Issues
- DNA and Nucleic Acid Chemistry
- French Historical and Cultural Studies
- Bioinformatics and Genomic Networks
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
- Diverse multidisciplinary academic research
- RNA and protein synthesis mechanisms
- Historical Studies and Socio-cultural Analysis
- Epigenetics and DNA Methylation
- Philippine History and Culture
Boston University
2018-2025
Janssen (United States)
2023
Collège de France
2014
Université Paris 8
2014
École Nationale Supérieure des Mines de Paris
2000
Enteric Escherichia coli survives the highly acidic environment of stomach through multiple acid resistance (AR) mechanisms. The most effective system, AR2, decarboxylates externally-derived glutamate to remove cytoplasmic protons and excrete GABA. first described AR1, does not require an external amino acid. Its mechanism has been determined. regulation AR systems their coordination with broader cellular metabolism fully explored.We utilized a combination ChIP-Seq gene expression analysis...
Abstract Bacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe approach to identify isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte develop these TFs into biosensor devices. Our utilizes combination genomic screens functional assays TFs, quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing recognition real-time quantitative measurements. use this progesterone-sensing aTF TF optical sensor...
Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells. However, scFv exhibit lower stability increased risk of aggregation due to transient dissociation ("breathing") inter-molecular reassociation the two (VL VH). We designed a novel strategy, referred stapling, that introduces disulfide bonds between linker minimize breathing. named...
Our understanding of the regulation gene expression has benefited from availability high-throughput technologies that interrogate whole genome for binding specific transcription factors and profiles. In case widely used model organisms, such as Escherichia coli K-12, new knowledge gained these approaches needs to be integrated with legacy accumulated genetic molecular biology experiments conducted in pre-genomic era order attain deepest level possible based on available data.In this paper,...
Phosphorothioate (PT) DNA modifications—in which a nonbonding phosphate oxygen is replaced with sulfur—represent widespread, horizontally transferred epigenetic system in prokaryotes and have highly unusual property of occupying only small fraction available consensus sequences genome. Using Salmonella enterica as model, we asked question fundamental importance: How do the PT-modifying DndA-E proteins select their G PS AAC/G TTC targets? Here, applied innovative analytical, sequencing,...
The DNA binding of most Escherichia coli Transcription Factors (TFs) has not been comprehensively mapped, and few have models that can quantitatively predict affinity. We report the global mapping in vivo for 139 E. TFs using ChIP-Seq. use these data to train BoltzNet, a novel neural network predicts TF energy from sequence. BoltzNet mirrors quantitative biophysical model provides directly interpretable predictions genome-wide at nucleotide resolution. design sites, which we validate with...
The DNA binding of most
L’analyse des usages d’amulettes ( patuás et mandingas ) dans la capoeira montre que ces formes du souci de soi renvoient aux techniques fabrication corps propres rites candomblé . Scarifié ouvert, pour être « fermé » protégé, le corpo fechado afro-brésilien est appréhendé en dehors clivages dualistes (âme/corps, contenu/contenant). Dans l’univers afro-brésilien, notion clôture se décline ainsi comme mise œuvre frontières.
Cet article se propose d'analyser la divination dans le Candomblé, religion brésilienne d'origine africaine, non pas à travers les seules données recueillies entretiens hors contexte avec des devins, mais aussi partir de description d'une séance comme situation communicationnelle. Il apparaît alors que logique divinatoire répond moins nécessité conférer un sens au désordre qu'à celle reconfigurer, par procédures pragmatiques, biographie du consultant. Le devin ne « devine » rien, consultant...
D’hoker, Elke & Stephanie Eggermont (eds.) The Irish Short Story – Traditions and Trends. Bern: Peter Lang, 2015. 322 pp.
En plaçant la fabrication rituelle au centre du dispositif de (re)production des humains et dieux, les initiés aux divinités brésiliennes d'origine africaine semblent minorer le rôle éminent « différence » sexuelle dans processus d'engendrement identités. Les sont agglomérats d'éléments nature investis par puissances ancestrales, trames relationnelles », différenciations qui adviennent à l'existence médiation manipulations rituelles d'une pluralité matières préexistantes. A partir l'étude...
Résumé Les rites sacrificiels et de possession caractérisant le candomblé ont longtemps été stigmatisés dans l’imaginaire la société brésilienne. Le processus, tout en tension, légitimation religieuse du couvre une période historique relativement courte – un siècle liée à l’émergence des supports communication masse. En effet, les relations adeptes médias sont complexes ambivalentes, constituées fois d’antagonismes d’éléments reconnaissance selon contextes périodes historiques :...