A5005255971- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- SARS-CoV-2 detection and testing
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Reproductive tract infections research
- SARS-CoV-2 and COVID-19 Research
- Orthopaedic implants and arthroplasty
- 3D Printing in Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Advanced Biosensing Techniques and Applications
- Syphilis Diagnosis and Treatment
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Bone Tissue Engineering Materials
- HIV Research and Treatment
- Metal and Thin Film Mechanics
- Urinary Tract Infections Management
- HIV/AIDS Research and Interventions
- Global Cancer Incidence and Screening
- COVID-19 epidemiological studies
- Diamond and Carbon-based Materials Research
- Electrospun Nanofibers in Biomedical Applications
- Mobile Health and mHealth Applications
Boston University
2016-2025
Bridge University
2014-2024
Stockholm University
2024
Mount Kenya University
2024
Kenyatta University
2024
University of Alabama at Birmingham
2017
Boston Biomedical (United States)
2015
Northeastern University
2012
Fraunhofer USA Center for Manufacturing Innovation
2010
Boston Medical Center
2008
Paper diagnostics have successfully been employed to detect the presence of antigens or small molecules in clinical samples through immunoassays; however, detection many disease targets relies on much higher sensitivity and specificity achieved via nucleic acid amplification tests (NAAT). The steps involved NAAT recently begun be explored paper matrices, our group, among others, has reported paper-based extraction, amplification, DNA RNA targets. Here, we integrate these into a single...
Significance Detection of submicroscopic malaria in asymptomatic individuals is needed for eradication and remains a diagnostic gap resource-limited settings. Nonfalciparum clinical diagnostics are second gap, as these infections have low parasite density commonly undetected. We describe an integrated, 60-min, ultrasensitive specific CRISPR-based the four major pathogenic Plasmodium species that can fill gaps. Using SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform,...
Sepsis caused by gram positive and negative bacteria is the leading cause of death in noncoronary ICUs tenth United States. We have developed a microfluidic sample preparation platform for rapid on-chip detection infectious organisms point-of-care diagnostics. The chips are made robust thermoplastic can be easily multiplexed high throughput applications. Bacteria lysed via hybrid chemical/mechanical method. Once lysed, bacterial DNA isolated using microscale silica bead/polymer composite...
The 2009 Influenza A (H1N1) pandemic disproportionately affected the developing world and highlighted key inadequacies of traditional diagnostic methods that make them unsuitable for use in resource-limited settings, from expensive equipment infrastructure requirements to unacceptably long turnaround times. While rapid immunoassay tests were much less costly more context-appropriate, they suffered drastically low sensitivities high false negative rates. An accurate, sensitive, specific...
Traditional methods for identifying pathogens in bacteremic patients are slow (24–48+ h). This can lead to physicians making treatment decisions based on an incomplete diagnosis and potentially increasing the patient's mortality risk. To decrease time diagnosis, we have developed a novel technology that recover viable bacteria directly from whole blood identify them less than 7 h. Our combines sample preparation process with surface-enhanced Raman spectroscopy (SERS). The enriches...
DNA extraction from clinical samples is commonly achieved with a silica solid phase column in the presence of chaotrope. Versions these protocols have been adapted for point care (POC) diagnostic devices miniaturized platforms, but commercial kits require high amount input DNA. Thus, when sample contains less than 1 μg total DNA, target-specific recovery most low without supplementing exogenous carrier In fact, many used development POC diagnostics often exhibit target concentrations as 3...
Sexually transmitted infections, including the human immunodeficiency virus (HIV) and papillomavirus (HPV), disproportionally impact those in low-resource settings. Early diagnosis is essential for managing HIV. Similarly, HPV causes nearly all cases of cervical cancer, majority (90%) which occur Importantly, infection with six times more likely to progress cancer women who are HIV-positive. An inexpensive, adaptable point-of-care test viral infections would make screening these viruses...
Abstract Background In January 2022, US guidelines shifted to recommend isolation for 5 days from symptom onset, followed by of mask-wearing. However, viral dynamics and variant vaccination impact on culture conversion are largely unknown. Methods We conducted a longitudinal study university campus, collecting daily anterior nasal swabs at least 10 reverse-transcription polymerase chain reaction (RT-PCR) testing culture, with antigen rapid diagnostic (RDT) subset. compared positivity beyond...
A polymeric microfluidic device for solid-phase extraction (SPE)-based isolation of nucleic acids is demonstrated. The plastic chip can function as a disposable sample preparation system different biological and diagnostic applications. was fabricated in cyclic polyolefin by hot-embossing with master mold. solid phase consisted porous monolithic polymer column impregnated silica particles. achieved due to the binding particles monolith. formed within channels situ photoinitiated...
The nanomechanical properties of various polymers were examined in light nanoindentation experiments performed with a diamond tip nominal radius curvature about 20 μm under conditions maximum contact load the range 150–600 μN and loading/unloading rates between 7.5 600 μN/s. elastic modulus each polymer was determined from unloading material response using compliance method, whereas hardness calculated as divided by corresponding projected area, obtained known shape function. It is shown...
Here we have demonstrated that radio frequency plasma and ultraviolet-ozone (UVO) surface modifications are effective treatments for enabling the thermal bonding of polymeric microfluidic chips at temperatures below T(g) (glass transition temperature) polymer. The effects UVO on properties a cyclic polyolefin polystyrene were examined with X-ray photoelectron spectroscopy (XPS), contact angle measurements, atomic force microscopy (AFM) roughness measurements adhesion AFM force-distance data....
In this paper, we present a fully integrated lab-on-a-chip and associated instrument for the detection of bacteria from liquid samples. The system conducts bacterial lysis, nucleic acid isolation concentration, polymerase chain reaction (PCR), end-point fluorescent detection. To enable truly low-cost manufacture single-use disposable chip, designed plastic chip in planar format without any active components to be amenable injection molding utilized novel porous polymer monolith (PPM)...
Cells of biomedical interest are, despite their functional significance, often present in very small numbers. Therefore the analysis and isolation previously inaccessible rare cells, such as peripheral hematopoietic stem endothelial progenitor or circulating tumor require efficient, sensitive, specific procedures that do not compromise viability cells. The current study builds on previous work a rationally designed microfluidic magnetophoretic cell separation platform capable throughputs 240...
Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point care detection sexually transmitted infections caused by Chlamydia trachomatis. This new platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual using only pressure source heat block, eliminating need expensive laboratory equipment. test can be performed in less than one hour has clinically relevant limit that is 100x more sensitive current rapid...
We report the first demonstration of using heat on a paper device to rapidly concentrate clinically relevant analyte interest from biological fluid. Our technology relies application localized strip evaporate off hundreds microliters liquid target analyte. This method can be used enrich for that is present at low concentrations within fluid enhance sensitivity downstream detection methods. demonstrate our by concentrating tuberculosis-specific glycolipid, lipoarabinomannan (LAM), promising...
Abstract Bacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe approach to identify isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte develop these TFs into biosensor devices. Our utilizes combination genomic screens functional assays TFs, quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing recognition real-time quantitative measurements. use this progesterone-sensing aTF TF optical sensor...
<h3>Importance</h3> The COVID-19 pandemic has severely disrupted US educational institutions. Given potential adverse financial and psychosocial effects of campus closures, many institutions developed strategies to reopen campuses in the fall 2020 semester despite ongoing threat COVID-19. However, opted have limited reopening minimize risk spread SARS-CoV-2. <h3>Objective</h3> To analyze how Boston University (BU) fully reopened its controlled transmission worsening Boston, Massachusetts....
Abstract Background The Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmissible in vaccinated and unvaccinated populations. dynamics that govern its establishment propensity toward fixation (reaching 100% frequency the SARS-CoV-2 population) communities remain unknown. Here, we describe at 3 institutions higher education (IHEs) greater Boston area. Methods We use diagnostic variant-specifying molecular assays epidemiological analytical...