A5015012628- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Nematode management and characterization studies
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Postharvest Quality and Shelf Life Management
- Plant Disease Management Techniques
- Banana Cultivation and Research
- Fungal Plant Pathogen Control
- Mycorrhizal Fungi and Plant Interactions
- Microtubule and mitosis dynamics
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Turfgrass Adaptation and Management
- Powdery Mildew Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Pressure Ulcer Prevention and Management
- Agricultural safety and regulations
- Seed Germination and Physiology
- Fermentation and Sensory Analysis
Planta
2021
Vlaams Instituut voor Biotechnologie
2004-2017
Ghent University
2004-2011
Institut de Biologie Moléculaire des Plantes
2004
To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential genes. More than 100 open reading frames were selected based on similarity to yeast animal genes, cytoskeleton polarity Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins determined by means GFP tagging tobacco Bright Yellow-2 cells plants. Detailed confocal microscopy identified 15...
Plant cells produce different microtubule arrays that are essential for cell division and morphogenesis without equivalent in other eukaryotes. Microtubule-associated proteins influence the behavior of microtubules is presumed to culminate into transitions from one array another. We analyzed microtubule-binding properties three Arabidopsis (Arabidopsis thaliana) members, AtMAP65-1, AtMAP65-4, AtMAP65-5, live using laser scanning confocal microscopy. Depending on overall organization cortical...
Cell cycle activity is required for plant growth and development, but its involvement in the early events that initiate seedling development remains to be clarified. We performed experiments aimed at understanding when cell progression activated during seed germination, what contribution proper establishment. To this end, spatial temporal expression profiles of a large set control genes germinating seeds Arabidopsis (Arabidopsis thaliana) white cabbage (Brassica oleracea) were analyzed. The...
Summary Giant cells induced by root knot nematodes and syncytia caused cyst are large multinucleated feeding containing a dense cytoplasm generated during complex host–parasite association in plant roots. To find out whether cytoskeleton changes occurred cell development, transcriptional activity of actin ( ACT ) tubulin genes organization the filaments microtubules (MTs) were analyzed situ . The importance architecture for proper initiation development galls was demonstrated perturbing with...
A multi-mycotoxin method was developed for the simultaneous determination of trichothecenes (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin, T-2 toxin), aflatoxins (aflatoxin-B(1), aflatoxin-B(2), aflatoxin-G(1) and aflatoxin-G(2)), Alternaria toxins (alternariol, alternariol methyl ether altenuene), fumonisins (fumonisin-B(1), fumonisin-B(2) fumonisin-B(3)), ochratoxin A, zearalenone, beauvericin...
During a survey of Phytophthora diversity in natural ecosystems Taiwan six new species were detected. Multigene phylogeny based on the nuclear ITS, ß-tubulin and HSP90 mitochondrial cox1 NADH1 gene sequences demonstrated that they belong to ITS Clade 7a with P. europaea , uniformis rubi cambivora being their closest relatives. All differed from each other related by unique combination morphological characters, breeding system, cardinal temperatures growth rates. Four homothallic species,...
Calonectria pseudonaviculata , the causal agent of disease Buxus spp. known as ‘box blight’, was first detected in mid‐1990s UK and New Zealand. Since then, geographic range box blight has rapidly expanded to at least 21 countries throughout temperate regions world, causing significant losses nurseries, gardens wild boxwood populations. This study determined genetic diversity a collection 234 isolates from diseased plants, originating 15 four continents. Two clades, G1 G2, were identified...
Abstract The genus Phytophthora comprises many economically and ecologically important plant pathogens. Hybrid species have previously been identified in at least six of the 12 phylogenetic clades. These hybrids can potentially infect a wider host range display enhanced vigour compared to their progenitors. therefore pose serious threat agriculture as well natural ecosystems. Early correct identification is essential for adequate protection but this hampered by limitations morphological...
Phytophthora pseudosyringae is a self-fertile pathogen of woody plants, particularly associated with tree species from the genera Fagus, Notholithocarpus, Nothofagus and Quercus, which found across Europe in parts North America Chile. It can behave as soil infecting roots stem collar region, well an aerial leaves, twigs barks, causing particular damage United Kingdom western America. The population structure, migration potential outcrossing worldwide collection isolates were investigated...
Wet sieving of soil samples, followed by plating on semi-selective medium and microscopic analysis, is the most commonly used technique to quantify microsclerotia-forming Verticillium species in soil. However, method restricted small does not allow easy differentiation between species, takes several weeks complete. This study describes an alternative test 100-g samples for three (V. tricorpus, V. dahliae, longisporum) using density flotation-based extraction microsclerotia new real-time...
Invasive, exotic plant pathogens pose a major threat to native and agricultural ecosystems. Phytophthora × cambivora is an invasive, destructive pathogen of forest fruit trees causing severe damage worldwide chestnuts (Castanea), apricots, peaches, plums, almonds cherries (Prunus), apples (Malus), oaks (Quercus), beech (Fagus). It was one the first damaging invasive species be introduced Europe North America, although its origin unknown. We determined population genetic history in Europe,...
Trichoderma asperellum Samuels, Lieckf. & Nirenberg is one of several species comprising effective biocontrol strains. One these, T34, shows activity against soilborne pathogens such as Rhizoctonia, Fusarium, and Pythium. The strains T34 depends on their establishment in the rhizosphere. Monitoring population other rhizosphere growing medium therefore important for evaluating potential different crops systems. Until now, strain-specific monitoring has been very difficult main quantification...
Abstract Fusarium oxysporum f. sp. lactucae race 4 causes vascular necrosis and wilting of lettuce. First observed in Belgium 2015, the lack disease resistance commercial cultivars allowed this pathogen to spread nearly entire Belgian production area within years. Different levels development were different greenhouses. To help explain variation, we collected 78 isolates characterized them both physiologically genetically. Molecular identification indicated that 91% belonged 4, while 6% 1,...
In this work the presence and migration behavior of mycotoxins formed in sweet pepper, inoculated by Fusarium species involved internal fruit rot, were investigated. Two different commercial pepper cultivars with two proliferatum isolates that sampled from diseased peppers. After 10 days incubation at 20 °C a closed container, lesion caused fungal infection was dissected. Around lesion, up to three concentric rings tissue width 5 mm cut out analyzed using multimycotoxin LC-MS/MS method. The...
In June 2020, during an observation of fruit-bearing hazelnut trees (Corylus avelana L., cv. Tonda Gentile), grown in a small private home garden the region Kavarna (North-Eastern Black Sea coast Bulgaria), symptoms powdery mildew were observed on each 15 trees. Compared to widely spread Bulgaria caused by Phyllactinia guttata and known with its patches differing chasmothecia exclusively formed abaxial leaf surface, newly disease was characterized presence concomitant pathogen's structures...
Fusarium oxysporum f.sp. lactucae (Fol) causes a vascular disease in lettuce that results significant yield losses. Race-specific and sensitive real-time PCR assays were developed for Fol races 1 4, which are prevalent Europe. Using genotyping-by-sequencing, unique DNA loci specific to each race identified subsequently used the design of primers hydrolysis probes. Two per ensure specificity. The two could be run duplex format, while still giving sensitivity 100 fg genomic all assays. Sample...
Cornelian cherry dogwood (Cornus mas) is a widespread species in Bulgaria and some cultivars with large fruits are the subject of propagation. In springs 2007 2008, severe, unusual damages were observed on sporadically scattered plantlets 'Kazanlashki' (known also as 'Kazanlaker') nursery located near Vratza northwestern Bulgaria. Symptoms identical both years expressed leaves, young shoots, adjacent rootstock wood. Dark brown, necrotic leaf spots initiated most often from periphery quickly...