A5037232061- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Biochemical and Molecular Research
- Parallel Computing and Optimization Techniques
- Distributed and Parallel Computing Systems
- Photoacoustic and Ultrasonic Imaging
- Multimedia Communication and Technology
- Cloud Computing and Resource Management
- Corneal surgery and disorders
- Advanced Biosensing Techniques and Applications
- Peer-to-Peer Network Technologies
- Quinazolinone synthesis and applications
- Caching and Content Delivery
- Pneumocystis jirovecii pneumonia detection and treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Real-Time Systems Scheduling
- Retinal Diseases and Treatments
Nanyang Technological University
1999-2023
Article7 December 2020Open Access Source DataTransparent process Dual inhibition of the terminal oxidases eradicates antibiotic-tolerant Mycobacterium tuberculosis Bei Shi Lee School Biological Sciences, Nanyang Technological University, Singapore, Singapore Search for more papers by this author Kiel Hards Department Microbiology and Immunology, Biomedical University Otago, Dunedin, New Zealand Maurice Wilkins Centre Molecular Biodiscovery, Auckland, Curtis A Engelhart Weill Cornell Medical...
Energy metabolism has recently gained interest as a target space for antibiotic drug development in mycobacteria. Of particular importance is bedaquiline (Sirturo), which kills mycobacteria by inhibiting the F1F0 ATP synthase. Other components of electron transport chain such NADH dehydrogenases (NDH-2 and NdhA) terminal respiratory oxidase bc1:aa3 are also susceptible to chemical inhibition. Because antituberculosis drugs prescribed part combination therapies, interaction between novel...
Abstract The approval of bedaquiline (BDQ) for the treatment tuberculosis has generated substantial interest in inhibiting energy metabolism as a therapeutic paradigm. However, it is not known precisely how BDQ triggers cell death Mycobacterium ( Mtb) . Using 13 C isotopomer analysis, we show that BDQ-treated Mtb redirects central carbon to induce metabolically vulnerable state susceptible genetic disruption glycolysis and gluconeogenesis. Metabolic flux profiles indicate dependent on ATP...
The influence of carbon metabolism on oxidative phosphorylation is poorly understood in mycobacteria. M. tuberculosis expresses two respiratory terminal oxidases, the cytochrome bc1:aa3 and bd oxidase, which are jointly required for mycobacterial viability. essentiality optimum growth illustrated by its vulnerability to chemical inhibition clinical drug candidate Q203 several other series. oxidase not strictly essential but maintain bioenergetics when function compromised. In this study, we...
Tuberculosis is an infectious disease that affected more than 50 million people and killed 6.7 patients in the past 5 years alone. Additionally, rising incidence of treatment resistance threatens global effort to eradicate this disease. With limited options available, additional novel antibiotics are needed for multidrug-resistant tuberculosis (MDR-TB). Telacebec a first-in-class antibiotic targets pathogen's energy metabolism.This paper provides overview recent progress development testing...
Screening for inhibitors of Cyt-<italic>bd</italic> in <italic>Mycobacterium bovis</italic> BCG and tuberculosis</italic> revealed thieno[3,2-<italic>d</italic>]pyrimidine (<bold>7</bold>) which through SAR efforts resulted an improved analogue (<bold>19</bold>) this scaffold.
Unicorn provides an architecture using Java to harness the vast processing power on Internet for distributed computing applications. It employs code-shipping paradigm broker this power. The client can either make use of suite applications provided by or upload his own server together with data be processed. These tasks are in parallel idle hosts logged server. A set programming tools, including Application Interface and Broker ServerLite (a scaled-down version Server), is clients facilitate...
The formation efficiency of hydride-induced Meisenheimer complexes nitroaromatic compounds is consistent with their anti-TB activities exemplied by MDL860 and benzothiazol <italic>N</italic>-oxide (BTO) analogs.
Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, permissible fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, contrast agent, enhance PAOM contrast. enables visualization deeper structures, such as choroidal vasculature sclera...
IP Multicast has been widely used for the distribution of real-time video. However, a large portion Internet is not multicast-enabled. Such networks rely on static relays and tunnels to support video streaming. Being static, streaming cannot readily adjust changing network conditions. This paper proposes an application level overlay using peering technology seamless ubiquitous multimedia across both multicast unicast networks. The comprises auto-configurable tree dynamic client incorporating...
Modern programming languages and operating systems encourage the use of threads to exploit concurrency simplify program structure. An integral important part Java language is its multithreading capability. Despite portability across almost all platforms, performance varies according support underlying system way Virtual Machine maps native threads. In this paper, a well-known compute-intensive benchmark, EP was used examine various issues involved in execution on two different multithreaded...
The development of cytochrome bd oxidase (cyt-bd) inhibitors are needed for comprehensive termination energy production in Mycobacterium tuberculosis (Mtb) to treat infections. Herein, we report on the structure-activity-relationships (SAR) 22 new N-phenethyl-quinazolin-4-yl-amines that target cyt-bd. Our focused set compounds was synthesized and screened against three mycobacterial strains: bovis BCG, H37Rv clinical isolate N0145 with without bcc:aa3 inhibitor Q203 an ATP depletion assay....