A5025319223- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Connexins and lens biology
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Trace Elements in Health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heat shock proteins research
- Advanced Glycation End Products research
- Endoplasmic Reticulum Stress and Disease
- Glutathione Transferases and Polymorphisms
- Genetic Neurodegenerative Diseases
- Metal-Catalyzed Oxygenation Mechanisms
- Alzheimer's disease research and treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Metalloenzymes and iron-sulfur proteins
- RNA Research and Splicing
- bioluminescence and chemiluminescence research
- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- Amyotrophic Lateral Sclerosis Research
- Porphyrin Metabolism and Disorders
- Renin-Angiotensin System Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genomics and Rare Diseases
Essen University Hospital
2024-2025
Universitätsmedizin Greifswald
2013-2022
Heinrich Heine University Düsseldorf
2016-2022
Universität Greifswald
2013-2022
Philipps University of Marburg
2010-2011
Karolinska Institutet
2006-2008
Nobel Foundation
2006
Significance Inflammation often complicates diseases associated with oxidative stress. This study shows that inflammatory macrophages release proteins specific forms of cysteine oxidation to disulfides, particularly glutathionylation. Redox proteomics identified peroxiredoxin 2 (PRDX2) as a protein released in glutathionylated form by inflammation both vivo and vitro. Extracellular PRDX2 then triggers the production TNF-α. These data indicate redox-dependent mechanisms, an cascade, can...
One of the promising possibilities clinical application cold plasma, so-called atmospheric plasma (CAP), is its on malignant cells and cancer tissue using anti-neoplastic effects, primarily through delivery reactive oxygen nitrogen species (ROS, RNS). In this study, we investigated impact CAP cellular proliferation consecutive molecular response mechanisms in established prostate (PC) cell lines. PC showed a significantly reduced growth following treatment as result both an immediate...
Nonclassical protein secretion is of major importance as a number cytokines and inflammatory mediators are secreted via this route. Current evidence indicates that there several mechanistically distinct methods nonclassical secretion. We have shown recently peroxiredoxin (Prdx) 1 Prdx2 released by various cells upon exposure to stimuli such lipopolysaccharide (LPS) or tumor necrosis factor alpha (TNF-α). The Prdx then acts induce production cytokines. However, Prdx1 2 do not signal peptides...
The mechanisms by which eukaryotic cells handle and distribute the essential micronutrient iron within cytosol other cellular compartments are only beginning to emerge. yeast monothiol multidomain glutaredoxins (Grx) 3 4 for both transcriptional regulation intracellular distribution. Despite fact that of metabolism differ drastically in fungi higher eukaryotes, conserved, yet their precise function vertebrates has remained elusive. Here we demonstrate a crucial role vertebrate-specific Grx3...
The proteins from the thioredoxin family are crucial actors in redox signaling and cellular response to oxidative stress. major intracellular source for oxygen radicals components of respiratory chain mitochondria. Here, we show that mitochondrial 2-Cys peroxiredoxin (Prx3) is not only substrate 2 (Trx2), but can also be reduced by glutaredoxin (Grx2) via dithiol reaction mechanism. Grx2 reduces Prx3 exhibiting catalytic constants (<i>K<sub>m</sub></i>, 23.8 μmol·liter<sup>−1</sup>;...
Human mitochondrial glutaredoxin (Grx2) was described as the first iron-sulfur protein from thioredoxin superfamily of proteins. The [2Fe-2S] cluster proposed to serve redox sensor for activation Grx2 during oxidative stress. authors have demonstrated that is complexed by two N-terminal active site thiols monomers and molecules glutathione are bound noncovalently proteins in equilibrium with solution. When reduced becomes limiting factor coordination, holo-Grx2 complex dissociates, yielding...
Abstract Many cell death pathways, including apoptosis, regulated necrosis, and ferroptosis, are relevant for neuronal share common mechanisms such as the formation of reactive oxygen species (ROS) mitochondrial damage. Here, we present role actin-regulating protein cofilin1 in regulating pathways oxidative death. Cofilin1 deletion HT22 cells exerted increased resilience, assessed by quantification ROS production, membrane potential, ATP levels. Further, cofilin1-deficient met their energy...
Charcot-Marie-Tooth (CMT) disease 4A is an autosomal-recessive polyneuropathy caused by mutations of ganglioside-induced differentiation-associated protein 1 (GDAP1), a putative glutathione transferase, which affects mitochondrial shape and alters cellular Ca2+ homeostasis. Here, we identify the underlying mechanism. We found that patient-derived motoneurons GDAP1 knockdown SH-SY5Y cells display two phenotypes: more tubular mitochondria metabolism characterized glutamine dependence fewer...
In the tumor host, neutrophils may exhibit protumor or antitumor activity. It is hypothesized that in response to host-derived therapy-induced factors, adopt diverse functional states ultimately execute these differential functions. Here, we provide an alternative scenario which of individual cell population determines overall versus outcome neutrophil‒tumor interactions. Experimentally, show human neutrophils, are sequentially stimulated with bacteria and secreted factors from cells, kill a...
Nucleoredoxin (Nrx) is an oxidoreductase of the thioredoxin family proteins. It was shown to act as a signal transducer in some pathways; however, so far, no comprehensive analysis its regulated substrates and functions available. Here, we used combination two different strategies fill this gap. First, analyzed thiol-redox state proteome SH-SY5Y neuroblastoma cells depleted Nrx compared control using differential thiol-labeling technique quantitative mass spectrometry. 171 proteins were...