A5040671188- Virus-based gene therapy research
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Protease and Inhibitor Mechanisms
- Glioma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Fibroblast Growth Factor Research
- Genetics, Bioinformatics, and Biomedical Research
- CRISPR and Genetic Engineering
- Sarcoma Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Protein Degradation and Inhibitors
- Rabies epidemiology and control
- Cancer-related gene regulation
- Kruppel-like factors research
- Toxin Mechanisms and Immunotoxins
- Bone Tumor Diagnosis and Treatments
- Glycosylation and Glycoproteins Research
Imperial College London
2015-2024
Hammersmith Hospital
2012-2024
The University of Texas MD Anderson Cancer Center
2004-2015
Qatar Foundation
2014
The London College
2010
Universität Hamburg
2009
University Medical Center Hamburg-Eppendorf
2009
George Washington University
2006
University of Liège
1995-2004
Membrane type 1 metalloprotease (MT1-MMP) is a transmembrane that plays major role in the extracellular matrix remodeling, directly by degrading several of its components and indirectly activating pro-MMP2. We investigated effects MT1-MMP overexpression on vitro vivo properties human breast adenocarcinoma MCF7 cells, which do not express or MMP-2. MMP-2 cDNAs were either transfected alone cotransfected. All clones overexpressing 1) able to activate endogenous exogenous pro-MMP-2, 2)...
We report a unique mutation in the D-amino acid oxidase gene (R199W DAO) associated with classical adult onset familial amyotrophic lateral sclerosis (FALS) three generational FALS kindred, after candidate screening 14.52 cM region on chromosome 12q22-23 linked to disease. Neuronal cell lines expressing R199W DAO showed decreased viability and increased ubiquitinated aggregates compared cells wild-type protein. Similarly, lentiviral-mediated expression of primary motor neuron cultures caused...
Endostatin and angiostatin are known as tumor‐derived angiogenesis inhibitors, but their mechanisms of action not yet completely defined. We report here that endostatin angiostatin, delivered by adenoviral vectors, reduced in vitro the neovessel formation mouse aortic ring assay 85 40%, respectively. also demonstrated vivo both inhibited local invasion tumor vascularization transplanted murine malignant keratinocytes, 50 90% development highly vascularized mammary tumors. This inhibition...
Background Under the direction and sponsorship of National Cancer Institute, we report on first pre-clinical trial Comparative Oncology Trials Consortium (COTC). The COTC is a novel infrastructure to integrate cancers that naturally develop in pet dogs into development path new human drugs. are designed address questions challenging conventional preclinical models early phase trials. Large animal spontaneous cancer can be valuable addition successful studies biology therapeutic drug, imaging...
Research Article27 February 2019Open Access Source DataTransparent process Efficacy of systemic temozolomide-activated phage-targeted gene therapy in human glioblastoma Justyna Magdalena Przystal Phage Therapy Group, Division Brain Sciences, Department Medicine, Imperial College London, UK Search for more papers by this author Sajee Waramit Md Zahidul Islam Pranjol Wenqing Yan Grace Chu Aitthiphon Chongchai Thailand Excellence Centre Tissue Engineering and Stem Cells, Biochemistry, Faculty...
Abstract BACKGROUND: Recently, considerable efforts have been directed toward antivascular therapy as a new modality to treat human cancers. However, targeting therapeutic gene of interest the tumor vasculature with minimal toxicity other tissues remains objective therapy. Tumor necrosis factor‐α (TNF‐α) is potent agent but has limited clinical utility because significant systemic toxicity. At maximum tolerated doses TNF‐α, there no meaningful antitumor activity. Hence, this study was...
Co-injection of fibroblasts with human epithelial breast-tumor MCF7 cells in the presence Matrigel enhances tumor growth nude mice. While most matrix metalloproteinases (MMPs) have been shown to be produced by stromal cells, such as are unable produce MMPs. We therefore, hypothesized that tumor-promoting effect could related their production In order inhibit proteases, over-production TIMP-2 was induced vitro retroviral-mediated gene transfer. TIMP-2-producing were then co-injected into...
Here we report in vitro and vivo detection of self-assembled Au−imidazole by using near-infrared surface-enhanced Raman scattering (NIR-SERS). In vivo, the structures were administered into tumor-bearing mice detected noninvasively. The complexes generated adsorption imidazole molecules onto Au nanoparticles (NP) then characterized as aqueous suspensions NIR-SERS, angle-dependent light with fractal dimension analysis, visible extinction spectroscopy. structure optical activity was sensitive...
Human sarcomas are rare but diverse malignant tumors derived from mesenchymal tissue. Clinical response to therapy is currently determined by the modified World Health Organization (WHO) criteria or Response Evaluation Criteria in Solid Tumors (RECIST), these standards correlate poorly with sarcoma patient outcome. We introduced ligand-directed particles elements of AAV and phage (AAVP) enable integration tumor targeting molecular imaging. report drug-response monitoring prediction a nude...
Adeno-associated virus/phage (AAVP) is a gene delivery vector constructed as hybrid between adeno-associated virus and filamentous phage. Tumor targeting following systemic administration has previously been demonstrated in several vivo cancer models, with tumor specificity achieved through display of an α(v) integrin-targeting ligand on the capsid. However, high titers AAVP are required for transduction large numbers mammalian cells. This study first to investigate mechanisms involved entry...
BACKGROUND Receptors in tumor blood vessels are attractive targets for ligand‐directed drug discovery and development. The authors have worked systematically to map human endothelial receptors (“vascular zip codes”) within tumors through direct peptide library selection cancer patients. Previously, they selected a ligand‐binding motif the interleukin‐11 receptor alpha (IL‐11Rα) vasculature. METHODS generated ligand‐directed, peptidomimetic (bone metastasis‐targeting peptidomimetic‐11...
Abstract Glioblastoma multiforme (GBM) remains a cancer with poor prognosis and few effective therapeutic options. Successful medical management of GBM is limited by the restricted access drugs to central nervous system (CNS) caused blood brain barrier (BBB). We previously showed that subset are arginine auxotrophic because transcriptional silencing ASS1 and/or ASL sensitive pegylated deiminase (ADI-PEG20). However, it unknown whether depletion in peripheral vivo has activity against...
Significance Over the past decade, we have used adeno-associated virus phage (AAVP) vectors for discovery, preclinical imaging, and translation therapy. However, hurdles remained with AAVP-mediated transgene delivery: both preinternalization (nonspecific protein adsorption, resulting in antibody-based neutralization) postinternalization (endolysosomal degradation). As a proof-of-concept, designed, developed, validated next-generation of targeted AAVP that mitigate these obstacles to...
Triple-negative breast cancer (TNBC) is an aggressive tumor with limited treatment options and poor prognosis. We applied the in vivo phage display technology to isolate peptides homing immunosuppressive cellular microenvironment of TNBC as a strategy for non-malignant target discovery. identified cyclic peptide (CSSTRESAC) that specifically binds vitamin D receptor, protein disulfide-isomerase A3 (PDIA3) expressed on cell surface tumor-associated macrophages (TAM), targets syngeneic TNBC,...