A5015939453- Biblical Studies and Interpretation
- Receptor Mechanisms and Signaling
- Historical and Linguistic Studies
- Archaeology and Historical Studies
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Nitric Oxide and Endothelin Effects
- Adenosine and Purinergic Signaling
- Phosphodiesterase function and regulation
- Development, Ethics, and Society
- Ion channel regulation and function
- Pharmacology and Obesity Treatment
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
- Vehicle emissions and performance
- Religion, Society, and Development
- Pentecostalism and Christianity Studies
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Systems Engineering Methodologies and Applications
- Transportation Planning and Optimization
- Military Strategy and Technology
- Simone de Beauvoir and Sartre
- Plant and animal studies
- Insect-Plant Interactions and Control
Lubbock Christian University
2011-2024
Smith College
2022
Northern Michigan University
2011
Arena Pharmaceuticals (United States)
2007
University of Alberta
2004
University of North Texas
1998-2003
University of North Texas Health Science Center
1998-2003
Texas Christian University
2002
The University of Texas at Arlington
2002
Centro de Información y Desarrollo de la Mujer
2002
5-Hydroxytryptamine (5-HT)(2C) receptor agonists hold promise for the treatment of obesity. In this study, we describe in vitro and vivo characteristics lorcaserin [(1R)-8-chloro-2,3,4,5-tetrahydro-1-methyl-1H-3 benzazepine], a selective, high affinity 5-HT(2C) full agonist. Lorcaserin bound to human rat receptors with (K(i) = 15 +/- 1 nM, 29 7 respectively), it was agonist functional inositol phosphate accumulation assay, 18- 104-fold selectivity over 5-HT(2A) 5-HT(2B) receptors,...
Book Review| April 01 2004 Progymnasmata: Greek Textbooks of Prose Composition and Rhetoric Rhetoric, George A. Kennedy. Michael W. Martin; Martin Search for other works by this author on: This Site Google Mikeal C. Parsons Journal Biblical Literature (2004) 123 (1): 180–183. https://doi.org/10.2307/3268567 Cite Icon Share Twitter Permissions Citation Martin, Parsons; Rhetoric. 1 January 2004; doi: Download citation file: Zotero Reference Manager EasyBib Bookends Mendeley Papers EndNote...
Pertussis toxin was used to examine the role of inhibitory guanine nucleotide regulatory protein, Ni, in muscarinic-receptor-mediated stimulation phosphoinositide turnover and calcium mobilization. In cultured chick heart cells, pertussis-toxin treatment inhibited attenuation isoprenaline-stimulated cyclic AMP accumulation. This finding is consistent with proposal that pertussis blocks capacity Ni couple muscarinic receptors adenylate cyclase. contrast, cells or 1321N1 human astrocytoma did...
The synthesis and SAR of a novel 3-benzazepine series 5-HT2C agonists is described. Compound 7d (lorcaserin, APD356) was identified as one the more potent selective compounds in vitro (pEC50 values functional assays measuring [3H]phosphoinositol turnover: = 8.1; 5-HT2A 6.8; 5-HT2B 6.1) an acute vivo rat food intake model upon oral administration (ED50 at 6 h 18 mg/kg). Lorcaserin further characterized single-dose pharmacokinetic study (t1/2 3.7 h; F 86%) 28-day weight gain growing...
The H1-histamine receptor antagonist [3H]mepyramine bound with high affinity (Kd = 3-5 nM) to membranes derived from 1321N1 human astrocytoma cells. H1-receptor antagonists triprolidine and diphenhydramine inhibited binding Kj values of 1-5 nM, whereas the H2-histamine cimetidine was greater than 100 microM. Histamine also cell membranes, histamine inhibition curve shifted right steepened in presence 1 microM guanosine 5'-O-(3-thiotriphosphate). Treatment cells pertussis toxin had no effect...
Activation of muscarinic cholinergic receptors 1321N1 human astrocytoma cells attenuates cyclic AMP accumulation. This effect results from an activation phosphodiesterase with no direct inhibition adenylate cyclase activity. In spite this lack coupling to cyclase, guanine nucleotides reduce the apparent binding affinity agonist carbachol in a washed membrane preparation cells. The order potency for is guanosine 5'-O-(3-thiotriphosphate) greater than 5'-guanylyl-imidodiphosphate = GTP GDP;...
It has been proposed elsewhere [Meeker, R.B. & Harden, T. K. (1982) Mol. Pharmacol. 22, 310-319] that muscarinic cholinergic receptor-mediated attenuation of cAMP accumulation occurs through activation phosphodiesterase in 1321N1 human astrocytoma cells. Pertussis toxin, which ADP-ribosylates the guanine nucleotide regulatory protein involved inhibition adenylate cyclase (Ni), utilized to further differentiate between mechanism regulation metabolism cells and involving other tissues....
A guanine-nucleotide-binding protein (G-protein) was purified from cholate extracts of bovine brain membranes by sequential DEAE-Sephacel, Ultrogel AcA-34, heptylamine-Sepharose and Sephadex G-150 chromatography. Guanosine 5′-[gamma-[35S]thio]triphosphate (GTP[35S])-binding activity copurified with a 25,000 Da peptide 35,000-36,000 doublet. Neither pertussis toxin nor cholera catalysed the ADP-ribosylation associated GTP[35S]-binding activity. Photoaffinity labelling 8-azido[gamma-32P]GTP...
Abstract Although strong evidence links the gut microbiome to metabolic disease, mechanisms linking microbiota hormonal and responses food are not well understood. After a meal, bacteria produce wide array of small molecule, protein, lipid metabolites originating from bacterial sources. Annotating physiological function select microbe-derived is critical understanding diet-microbe-host interactions, developing microbiome-inspired therapies improve human health. Here, we have investigated...
Inhibitory coupling of receptors to adenylate cyclase previously has been shown be relatively sensitive inactivation by alkylation with N-ethylmaleimide (NEM). Modification the inhibitory guanine nucleotide regulatory protein, Ni, proposed responsible for this effect. The effects NEM on GTP-sensitive binding carbachol muscarinic cholinergic compared in a cell line (1321N1 human astrocytoma cells) which these stimulate phosphoinositide breakdown and (NG108-15 neuroblastoma X glioma activation...
The rat Sertoli cell in culture expresses Ai inhibitory adenosine receptors. In this study, we have used pertussis toxin as a tool to characterize the mechanism of action on these cells. Cells were preincubated for 18-24 h with toxin, and responses FSH analog phenylisopropyladenosine (PIA) measured by assaying cAMP accumulation. effect receptors was also evaluated measuring binding agonist cyclohexyladenosine (CHA). total number specific CHA-binding sites reduced 60–70% membranes prepared...
Agonist occupation of muscarinic cholinergic receptors 1321N1 human astrocytoma cells results in an activation phosphodiesterase and a resultant 50-75% attenuation isoproterenol-stimulated cyclic AMP accumulation. The effects series inhibitors on receptor-mediated inhibition accumulation the activities partially purified, soluble have been compared to determine which form activity is regulated by receptors. (50 microM) 1-methyl-3-isobutylxanthine (MIX), 1-methyl-3-isobutyl-7-benzylxanthine...
Abstract: In this study I argue that OG Isaiah in its rendering of chaps. 48–49 presents the servant as both a true individual and Lord's messianic agent world. chap. 48, recasts clearly collectivist "servant Jacob" an distinct from nation Moses-like new exodus. 49, enhances clarifies features suggestive individualist interpretation (contrary to scholarly opinion on matter), thereby aligning 49 more with 48. Further, course individuating servant, transfers aspects Israel's Yhwh's...
Abstract A guanine nucleotide-dependent P2Y-purinergic receptor-regulated phospholipase C activity of turkey erythrocyte membranes has been characterized in detail previously (Boyer, J. L., Downes, C. P., and Harden, T. K. (1989) Biol. Chem. 264, 884-890). The occurrence agonist-induced desensitization this is now described. Preincubation erythrocytes with the receptor agonist ADP beta S resulted a marked loss capacity plus GTP to stimulate derived from these cells. half-time was 0.5-2.0...
Modulation of the affinity agonists and antagonists at muscarinic cholinergic receptors in rat heart membranes was investigated using radiolabeled antagonist, [3H]quinuclidinyl benzilate ([3H]QNB), agonist, [methyl-3H]oxotremorine acetate ([3H]OXO). Receptor for oxotremorine measured competition binding assays with [3H]QNB or by equilibrium [3H]OXO increased when incubation temperature reduced to 4 degrees C. In contrast, receptor decreased lower temperatures a marked effect guanine...
The interaction of a radiolabeled muscarinic cholinergic receptor agonist, [methyl-3H]oxotremorine acetate [( 3H]OXO), with washed membrane preparation derived from rat heart, has been studied. In binding assays at 4 degrees C, the rate constants for association and dissociation [3H]OXO were 2 X 10(7) M-1 min-1 5 10(-3) min-1, respectively, Saturation isotherms indicated that was to single population sites Kd approximately 300 pM. density (90-100 fmol/mg protein) 75% determined antagonist...
The properties of an (125)I-labeled structural analog 2, 3-dimethoxy-N-[9-(4-fluorobenzyl)-9-azabicyclo[3.3. 1]nonan-3beta-yl]benzamide (MABN), (125)I-IABN, are described. (125)I-IABN was developed as a high-affinity radioligand selective for the D2-like (D2, D3, and D4) dopamine receptor subtypes. binds with picomolar affinity nonselectively to rat D2 D3 receptors expressed in Sf9 HEK 293 cells. 7- 25-fold lower human D4.4 Dissociation constants (Kd) calculated from kinetic experiments were...
This study examines comparison in Hebrews the light of ancient rhetorical theory syncrisis, identifying five epideictic syncrises: messengers/angels vs. Jesus (1.1-14; 2.5-18), Moses (3.1-6), Aaronic high priests (5.1-10), Levitical priestly ministry Melchizedekian (7.1-10.18), and Mt. Sinai Zion (12.18-24). The shows that these comparisons collectively function as a single syncritical project argues for superiority new covenant to old, project, like most individual comparisons, is arranged...
This study challenges the view that Heb 5:11-6:20 is a “digression”—a so widely held as to be near consensus in scholarship today, and renders controversial materials of chapter six tangential central purposes speech. The gives consideration ancient rhetorical theory concerning digressio , surveying major handbooks discuss figure. On basis this survey, argues only 5:11-14 displays essential characteristics . Moreover, its position function, actually points speech’s cause.
This study examines deliberative syncrisis and its relationship to epideictic in Hebrews, with a view addressing the question of whether rhetoric Hebrews is ultimately or overall tenor aim. Building on previous argues that consistently service syncrisis, providing it both logical premise topical theme by which advances argument. key, argues, Hebrews' structure aim, are decidedly nature.